Improved human sperm recovery using superoxide dismutase and catalase supplementation in semen cryopreservation procedure

Cell and Tissue Banking

Volumn 2, Issue 1, 2001, Pages 9-13

  Rossi, Tiziana ^a   Mazzilli, Fernando ^a   Delfino, Michele ^a   Dondero, Franco ^a  

^a POLICLINICO UMBERTO I   (Italy)

Author keywords

Catalase; Cryopreservation; Reactive oxygen species (ROS); Semen; Superimposed image analysis system; Superoxide dismutase (SOD)

Indexed keywords

BUFFER; CATALASE; HYDROGEN PEROXIDE; MEMBRANE LIPID; REACTIVE OXYGEN METABOLITE; SCAVENGER; SUPEROXIDE; SUPEROXIDE DISMUTASE;

ANDROLOGY; ARTICLE; CELL VIABILITY; CONTROLLED STUDY; CRYOPRESERVATION; FREEZING; HUMAN; HUMAN CELL; INFERTILITY; LEUKOCYTE COUNT; LIPID PEROXIDATION; OSMOSIS; PRIORITY JOURNAL; SAMPLE; SCREENING; SEMEN ANALYSIS; SPERM; SPERMATOZOON COUNT; SPERMATOZOON MOTILITY; SUPPLEMENTATION; THAWING; VISCOSITY;

EID: 0034888842     PISSN: 13899333     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1011592621487     Document Type: Article

References (35)

1. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa
2. Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques
3. Analysis of the relationship between defective sperm function and the generation of reactive oxygen species in cases of oligozoospermia
4. Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as a major enzyme protectant against oxygen toxicity
5. Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as mode of sublethal cryodamage to human sperm during cryopreservation
6. Evidence that membrane stress contributes more than lipid peroxidation to sublethal cryodamage in cryopreserved human sperm: Glicerol and others polyols as sole cryoprotectant
7. Effect of natural antioxidants tocopherol and ascorbic acids in maintenance of sperm activity during freeze-thaw process
8. Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing
9. Sperm adenosine triphosphate concentrations before and after freezing as a marker of cryolysis
10. Assessment of sperm for cryopreservation using the hypoosmotic viability test
11. Spontaneous production of reactive oxygen species (ROS) by human motile spermatozoa
12. Cold shock damage is due to lipid phase transitions in cell membranes: A demonstration using sperm as a model
13. Effects of antioxidants on human sperm preparation techniques
14. Formation of reactive oxygen species in spermatozoa of infertile patients
15. Catalase activity in human spermatozoa and seminal plasma
16. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics
17. Peroxidative breakdown of phospholipids in human spermatozoa, spermicidal properties of fatty acid peroxides, and protective action of seminal plasma
18. Semen analysis data from fresh and cryopreserved donor ejaculates: Comparison of cryoprotectants and pregnancy rates
19. Protective role of superoxide dismutase in human sperm motility: Superoxide dismutase activity and lipid peroxide in human seminal plasma and spermaozoa
20. Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation
21. Superoxide anion in human semen related to seminal parameters and clinical aspects
22. Superimposed image analysis system (SIAS) software: A new approach to sperm motility assessment
23. Reactive oxygen species (ROS) by human spermatozoa before and after cryopreservation
24. Application of the upgraded image superimposition system (SIAS) to the assessment of sperm kinematics
25. Superoxide dismutase in human semen
26. Increase of the molecular rigidity of the protein conformation in the intestinal brushborder membranes by lipid peroxidation
27. A comparative study of buffer systems as cryoprotectants for human spermatozoa
28. Improved recovery of post-thaw motility and vitality of human spermatozoa cryopreserved in the presence of dithiothreitol
29. Improvement of post-thaw sperm motility in poor quality human semen
30. Improved methods of preservation of human spermatozoa by freezing and freeze-drying
31. Extracellular presence of reactive oxygen species (ROS) in fresh and frozen-thawed canine semen and their effects on some semen parameters
32. Platelet activating factor and pentoxifylline as human sperm cryoprotectants
33. Reactive oxygen species generation by seminal cells during cryopreservation