Large-scale production, purification and bioactivity assay of recombinant human interleukin-6 in the methylotrophic yeast Pichia pastoris

FEMS Yeast Research

Volumn 11, Issue 2, 2011, Pages 160-167

  Li, Hongbo ^a   Wang, Yu ^a,b   Xu, Aimin ^a,b   Li, Shiwu ^c   Jin, Shouguang ^c   Wu, Donghai ^a  

^a GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH   (China)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

^c UNIVERSITY OF FLORIDA   (United States)

Author keywords

Expression and purification; High density fermentation; Interleukin 6; Pichia pastoris; Recombinant protein

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ANION; DNA FRAGMENT; INTERLEUKIN 6; MACROGOL 8000; METHANOL; RECOMBINANT PROTEIN; SEPHADEX;

AMINO ACID SEQUENCE; ARTICLE; ASSAY; CELL ASSAY; CLONE; DRUG ACTIVITY; DRUG MANUFACTURE; DRUG PURIFICATION; ESCHERICHIA COLI; GEL FILTRATION; GENE SEQUENCE; GENOME; HUMAN; LARGE SCALE PRODUCTION; LYMPHOCYTE; LYMPHOCYTE PROLIFERATION; MASS SPECTROMETRY; METHYLOTROPHIC YEAST; MOLECULAR WEIGHT; NONHUMAN; PICHIA PASTORIS; PRECIPITATION;

ESCHERICHIA COLI; PICHIA PASTORIS;

EID: 79951481535     PISSN: 15671356     EISSN: 15671364     Source Type: Journal    
DOI: 10.1111/j.1567-1364.2010.00701.x     Document Type: Article

References (25)

1. Arcone R, Pucci P, Zappacosta F, Fontaine V, Malorni A, Marino G & Ciliberto G (1991) Single-step purification and structural characterization of human interleukin-6 produced in Escherichia coli from a T7 RNA polymerase expression vector. Eur J Biochem 198: 541-547.
2. Atef A, Imen R, Koussay D & H'ela K (2008) High level production and purification of human interferon α2b in high cell density culture of Pichia pastoris. Enzyme Microb Technol 42: 173-180.
3. Becker C, Fantini M, Schramm C, Lehr H, Wirtz S, Nikolaev A, Burg J, Strand S, Kiesslich R & Huber S (2004) TGFβ suppresses tumor progression in colon cancer by inhibition of IL-6 trans-signaling. Immunity 21: 491-501.
4. Blay JY, Negrier S, Combaret V et al. (1992) Serum level of interleukin 6 as a prognosis factor in metastatic renal cell carcinoma. Cancer Res 52: 3317-3322.
5. Bommert K, Bargou RC & Stuhmer T (2006) Signalling and survival pathways in multiple myeloma. Eur J Cancer 42: 1574-1580.
6. Brady CP, Shimp RL, Miles AP, Whitmore M & Stowers AW (2001) High-level production and purification of P30P2MSP119, an important vaccine antigen for malaria, expressed in the methylotrophic yeast Pichia pastoris. Prot Exp Purif 23: 468-475.
7. Cereghino JL & Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24: 45-66.
8. Cozen W, Gill PS, Ingles SA et al. (2004) IL-6 levels and genotype are associated with risk of young adult Hodgkin lymphoma. Blood 103: 3216-3221.
9. Cregg JM, Tschopp JF, Stillman C, Siegel R, Akong M, Craig WS, Buckholz RG, Madden KR & Kellaris PA (1987) High level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris. Bio Technol 59: 479-485.
10. Erik B, Gerhard S, Andrea M, Hans-Peter M, Gerd G & Gotthard K (2007) Production of interleukin-6 in Arxula adeninivorans, Hansenula polymorpha and Saccharomyces cerevisiae by applying the wide-range yeast vector (CoMedTM) system to simultaneous comparative assessment. FEMS Yeast Res 7: 1181-1187.
11. Febbraio MA & Pedersen BK (2005) Contraction-induced myokine production and release: is skeletal muscle an endocrine organ? Exerc Sport Sci Rev 33: 114-119.
12. Fonseca JE, Santos MJ, Canháo H & Choy E (2009) Interleukin-6 as a key player in systemic inflammation and joint destruction. Autoimmun Rev 8: 538-542.
13. Hu ZY, Lai WS, Zhang WZ & Deng N (2007) Secretion expression and activity assay of a novel fusion protein of thrombopoietin and interleukin-6 in Pichia pastoris. J Biochem 142: 17-24.
14. Isomoto H, Mott JL, Kobayashi S, Werneburg NW, Bronk SF, Haan S & Gores JG (2007) Sustained IL-6/STAT-3 signaling in cholangiocarcinoma cells due to SOCS-3 epigenetic silencing. Gastroenterology 132: 384-396.
15. Jahic M, Veide A, Charoenrat T, Teeri T & Enfors SO (2006) Process technology for production and recovery of heterologous proteins with Pichia pastoris. Biotechnol Prog 22: 1465-1473.
16. Kamimura D, Ishihara K & Hirano T (2003) IL-6 signal transduction and its physiological roles: the signal orchestration model. Rev Physiol Biochem Pharmacol 149: 1-38.
17. Kanazawa T, Nishino H, Hasegawa M, Ohta Y, Iino Y, Ichimura K & Noda Y (2007) Interleukin-6 directly influences proliferation and invasion potential of head and neck cancer cells. Eur Arch Otorhinolaryngol 264: 815-821.
18. Kishimoto T (1989) The biology of interleukin-6. Blood 74: 1-10.
19. Kishimoto T (2005) Interleukin-6: from basic science to medicine - 40 years in immunology. Annu Rev Immunol 23: 1-21.
20. Kishimoto T, Akira S, Narazaki M & Taga T (1995) Interleukin-6 family of cytokines and gp130. Blood 86: 1243-1254.
21. Okada K, Shimizu Y, Nambu S, Higuchi K & Watanabe A (1994) Interleukin-6 functions as an autocrine growth factor in a cholangiocarcinoma cell line. J Gastroenterol Hepatol 9: 462-467.
22. Petrovay F, Heltai K, Kis Z, Treso B, Gonczol E, Burian K, Endresz V & Valyi-Nagy I (2007) Chronic infections and histamine, CRP and IL-6 levels after percutaneous transluminal coronary angioplasty. Inflamm Res 56: 362-367.
23. Rock FL, Li X, Chong P, Ida N & Klein M (1994) Roles of disulfides bonds in recombinant human interleukin-6 conformation. Biochemistry 33: 5146-5154.
24. Somers W, Stahl M & Seehra JS (1997) 1.9A crystal structure of interleukin 6: implications for a novel mode of receptor dimerization and signaling. EMBO J 16: 989-997.
25. Van de Loosdrecht AA, Beelen RH, Ossenkoppele GJ, Broekhoven MG & Langenhuijsen MM (1994) A tetrazolium based colorimetric MTT assay to quantitate human monocyte mediated cytotoxicity against leukemic cells from cell lines and patients with acute myeloid leukemia. J Immunol Methods 174: 311-330.