Nup2p dynamically associates with the distal regions of the yeast nuclear pore complex

Journal of Cell Biology

Volumn 153, Issue 7, 2001, Pages 1465-1478

  Dilworth, David J ^a,b   Suprapto, Adisetyantari ^c   Padovan, Julio C ^c   Chait, Brian T ^c   Wozniak, Richard W ^b   Rout, Michael P ^b   Aitchison, John D ^a,b  

^a INSTITUTE FOR SYSTEMS BIOLOGY   (United States)

^b UNIVERSITY OF ALBERTA   (Canada)

^c ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Exportin; Importin; Karyopherin; Nuclear transport; Nucleoporin

Indexed keywords

GUANOSINE TRIPHOSPHATE; KARYOPHERIN; NUCLEOPORIN; PROTEIN KAP60P; PROTEIN NUP2P; PROTEIN NUP60P; RAN PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL NUCLEUS; CONTROLLED STUDY; CYTOLYSIS; CYTOPLASM; HETEROKARYON; NONHUMAN; NUCLEOCYTOPLASMIC TRANSPORT; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN METABOLISM; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; YEAST;

BETA KARYOPHERINS; BINDING SITES; CELL NUCLEUS; CYTOPLASM; DEOXYGLUCOSE; FUNGAL PROTEINS; GREEN FLUORESCENT PROTEINS; GUANOSINE TRIPHOSPHATE; HEAT-SHOCK PROTEINS; LUMINESCENT PROTEINS; NUCLEAR PORE; NUCLEAR PORE COMPLEX PROTEINS; NUCLEAR PROTEINS; PORINS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; RAN GTP-BINDING PROTEIN; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE PROTEINS; SODIUM AZIDE; STAPHYLOCOCCUS AUREUS;

EID: 0035954435     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.153.7.1465     Document Type: Article

References (61)

1. Nup120p: A yeast nucleoporin required for NPC distribution and mRNA transport
2. Two novel related yeast nucleoporins Nup170p and Nup157p: Complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p
3. Kap104p: A karyopherin involved in the nuclear transport of messenger RNA binding proteins
4. Structural plasticity of the nuclear pore complex
5. The nuclear pore complex: Mediator of translocation between nucleus and cytoplasm
6. Nuclear PRP20 protein is required for mRNA export
7. Structural basis for the interaction between FxFG nucleoporin repeats and importin-β in nuclear trafficking
8. Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p
9. Unidirectional and bidirectional protein traffic across membranes
10. The yeast nucleoporin Nup2p is involved in nuclear export of importin α/Srp1p
11. In vivo dynamics of nuclear pore complexes in yeast
12. Nucleocytoplasmic transport: Driving and directing transport
13. Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin α
14. Mapping interactions between nuclear transport factors in living cells reveals pathways through the nuclear pore complex
15. A structure/function analysis of Rat7p/Nup159p, an essential nucleoporin of Saccharomyces cerevisiae
16. A family of Ran binding proteins that includes nucleoporins
17. cDNA cloning and characterization of Npap60: A novel rat nuclear pore-associated protein with an unusual subcellular localization during male germ cell differentiation
18. The nucleoporin Nup98 is a site for GDP/GTP exchange on ran and termination of karyopherin β 2-mediated nuclear import
19. CRM1 is an export receptor for leucine-rich nuclear export signals
20. Identification of different roles for RanGDP and RanGTP in nuclear protein import
21. Transport between the cell nucleus and the cytoplasm
22. Nup50, a nucleoplasmically oriented nucleoporin with a role in nuclear protein export
23. Evolutionary conservation of components of the protein translocation complex
24. + RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene
25. Nup2p is located on the nuclear side of the nuclear pore complex and coordinates Srp1p/importin-α export
26. A role for RanBP1 in the release of CRMI from the nuclear pore complex in a terminal step of nuclear export
27. Effect of paraformaldehyde fixation on localization and characterization of insulin-like growth factor-I (IGF-I) receptors in the rat brain
28. The essential yeast nucleoporin NUP159 is located on the cytoplasmic side of the nuclear pore complex and serves in karyopherin-mediated binding of transport substrate
29. Yeast Ran-binding protein 1 (Yrb1) shuttles between the nucleus and cytoplasm and is exported from the nucleus via a CRM1 (XPO1)-dependent pathway
30. NUP2, a novel yeast nucleoporin, has functional overlap with other proteins of the nuclear pore complex
31. Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p
32. Nucleocytoplasmic transport: The soluble phase
33. Ran and nuclear transport
34. GLE2, a Saccharomyces cerevisiae homologue of the Schizosaccharomyces pombe export factor RAE1, is required for nuclear pore complex structure and function
35. Role of the nuclear transport factor p10 in nuclear import
36. The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex
37. Transport routes through the nuclear pore complex
38. Protein import into nuclei: Association and dissociation reactions involving transport substrate, transport factors, and nucleoporins
39. The nulcear pore complex as a transport machine
40. The yeast nuclear pore complex: Composition, architecture, and transport mechanism
41. Isolation of the yeast nuclear pore complex
42. A distinct nuclear import pathway used by ribosomal proteins
43. The nuclear pore complex: A protein machine bridging the nucleus and cytoplasm
44. Yeast heat shock mRNAs are exported through a distinct pathway defined by Rip1p
45. In vivo nuclear transport kinetics in Saccharomyces cerevisiae: A role for heat shock protein 70 during targeting and translocation
46. Characterization and targeted disruption of murine Nup50, a p27(Kipl)-interacting component of the nuclear pore complex
47. Nup2p, a yeast nucleoporin, functions in bidirectional transport of importin α
48. Exportin 1 (Crm1p) is an essential nuclear export factor
49. The nuclear pore complex: From molecular architecture to functional dynamics
50. Proteins connecting the nuclear pore complex with the nuclear interior
51. Yrb2p is a nuclear protein that interacts with Prp20p, a yeast Rcc1 homologue
52. A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export
53. Nucleus-associated pools of Rnalp, the Saccharomyces cerevisiae Ran/TC4 GTPase activating protein involved in nucleus/cytosol transit
54. Separate domains of KAR1 mediate distinct functions in mitosis and nuclear fusion
55. Identification of hSRPI α as a functional receptor for nuclear localization sequences
56. Gatekeepers of the nucleus
57. Karyopherins and kissing cousins
58. Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region
59. Yeast Srp1p has homology to armadillo/plakoglobin/β-catenin and participates in apparently multiple nuclear functions including the maintenance of the nucleolar structure
60. GTP hydrolysis links initiation and termination of nuclear import on the nucleoporin Nup358
61. Two distinct classes of Ran-binding sites on the nucleoporin Nup 358