Effect of desacyl ghrelin, obestatin and related peptides on triglyceride storage, metabolism and GHSR signaling in 3T3-L1 adipocytes

Journal of Cellular Biochemistry

Volumn 112, Issue 2, 2011, Pages 704-714

  Miegueu, Pierre ^a   St Pierre, David ^b   Broglio, Fabio ^b   Cianflone, Katherine ^a  

^a LAVAL UNIVERSITY   (Canada)

^b UNIVERSITY OF TURIN   (Italy)

Author keywords

desacyl ghrelin; ghrelin; GHSR receptor; obestatin; triglyceride storage

Indexed keywords

ACYLGHRELIN; DESACYLGHRELIN; GHRELIN; GLUCOSE; GROWTH HORMONE SECRETAGOGUE RECEPTOR; INSULIN; OBESTATIN; TRIACYLGLYCEROL; UNCLASSIFIED DRUG;

ADIPOCYTE; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL METABOLISM; CELL PROLIFERATION; CELL STIMULATION; CONTROLLED STUDY; CORRELATION ANALYSIS; GENE EXPRESSION; GLUCOSE TRANSPORT; LIPID STORAGE; LIPID TRANSPORT; LIPOLYSIS; MICROARRAY ANALYSIS; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SUBCUTANEOUS FAT; UNINDEXED SEQUENCE;

3T3-L1 CELLS; ADIPOCYTES; ADULT; ANIMALS; BIOLOGICAL TRANSPORT; CELL PROLIFERATION; GHRELIN; GLUCOSE; HUMANS; MALE; MICE; MIDDLE AGED; PEPTIDES; RECEPTORS, GHRELIN; TRIGLYCERIDES;

EID: 79251634954     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.22983     Document Type: Article

References (49)

1. Allende-Vigo MZ,. 2010. Adipocytes and Cardiometabolic Risk. Endocr Pract 3: 1-21.
2. Asakawa A, Inui A, Fujimiya M, Sakamaki R, Shinfuku N, Ueta Y, Meguid MM, Kasuga M,. 2005. Stomach regulates energy balance via acylated ghrelin and desacyl ghrelin. Gut 54 (1): 18-24.
3. Baldanzi G, Filigheddu N, Cutrupi S, Catapano F, Bonissoni S, Fubini A, Malan D, Baj G, Granata R, Broglio F, Papotti M, Surico N, Bussolino F, Isgaard J, Deghenghi R, Sinigaglia F, Prat M, Muccioli G, Ghigo E, Graziani A,. 2002. Ghrelin and des-acyl ghrelin inhibit cell death in cardiomyocytes and endothelial cells through ERK1/2 and PI 3-kinase/AKT. J Cell Biol 159 (6): 1029-1037.
4. Boney CM, Gruppuso PA, Faris RA, Frackelton AR, Jr., 2000. The critical role of Shc in insulin-like growth factor-I-mediated mitogenesis and differentiation in 3T3-L1 preadipocytes. Mol Endocrinol 14 (6): 805-813.
5. Cassoni P, Papotti M, Ghà C, Catapano F, Sapino A, Graziani A, Deghenghi R, Reissmann T, Ghigo E, Muccioli G,. 2001. Identification, characterization, and biological activity of specific receptors for natural (ghrelin) and synthetic growth hormone secretagogues and analogs in human breast carcinomas and cell lines. J Clin Endocrinol Metab 86 (4): 1738-1745.
6. Cassoni P, Ghé C, Marrocco T, Tarabra E, Allia E, Catapano F, Deghenghi R, Ghigo E, Papotti M, Muccioli G,. 2004. Expression of ghrelin and biological activity of specific receptors for ghrelin and des-acyl ghrelin in human prostate neoplasms and related cell lines. Eur J Endocrinol 150 (2): 173-184.
7. Catalan V, Gomez-Ambrosi J, Rotellar F, Silva C, Gil MJ, Rodriguez A, Cienfuegos JA, Salvador J, Fruhbeck G,. 2007. The obestatin receptor (GPR39) is expressed in human adipose tissue and is down-regulated in obesity-associated type 2 diabetes mellitus. Clin Endocrinol (Oxf) 66: 598-601.
8. Chen CY, Asakawa A, Fujimiya M, Lee SD, Inui A,. 2009. Ghrelin gene products and the regulation of food intake and gut motility. Pharmacol Rev 61 (4): 430-481.
9. Choi K, Roh SG, Hong YH, Shrestha YB, Hishikawa D, Chen C, Kojima M, Kangawa K, Sasaki S,. 2003. The role of ghrelin and growth hormone secretagogues receptor on rat adipogenesis. Endocrinology 144 (3): 754-759.
10. Colinet FG, Vanderick S, Charloteaux B, Eggen A, Gengler N, Renaville B, Brasseur R, Portetelle D, Renaville R,. 2009. Genomic location of the bovine growth hormone secretagogue receptor (GHSR) gene and investigation of genetic polymorphism. Anim Biotechnol 20 (1): 28-33.
11. Cui W, Lapointe M, Gauvreau D, Kalant D, Cianflone K,. 2009. Recombinant C3adesArg/acylation stimulating protein (ASP) is highly bioactive: A critical evaluation of C5L2 binding and 3T3-L1 adipocyte activation. Mol Immunol 46 (16): 3207-3217.
12. Dass NB, Hill J, Muir A, Testa T, Wise A, Sanger GJ,. 2003. The rabbit motilin receptor: Molecular characterisation and pharmacology. Br J Pharmacol 145 (5): 948-954.
13. Davies JS, Kotokorpi P, Eccles SR, Barnes SK, Tokarczuk PF, Allen SK, Whitworth HS, Guschina IA, Evans BA, Mode A, Zigman JM, Wells T,. 2009. Ghrelin induces abdominal obesity via GHS-R-dependent lipid retention. Mol Endocrinol 23 (6): 914-924.
14. Druce MR, Neary NM, Small CJ, Milton J, Monteiro M, Patterson M, Ghatei MA, Bloom SR,. 2006. Subcutaneous administration of ghrelin stimulates energy intake in healthy lean human volunteers. Int J Obes (Lond) 30 (2): 293-296.
15. Epelbaum J, Bedjaoui N, Dardennes R, Feng DD, Gardette R, Grouselle D, Loudes C, Simon A, Tolle V, Yang SK, Zizzari P,. 2010. Role of the ghrelin/obestatin balance in the regulation of neuroendocrine circuits controlling body composition and energy homeostasis. Mol Cell Endocrinol 314 (2): 244-247.
16. Fontenot E, DeVente JE, Seidel ER,. 2007. Obestatin and ghrelin in obese and in pregnant women. Peptides 28 (10): 1937-1944.
17. Gauna C, Delhanty PJ, Hofland LJ, Janssen JA, Broglio F, Ross RJ, Ghigo E, Van Der Lely AJ,. 2005. Ghrelin stimulates, whereas des-octanoyl ghrelin inhibits, glucose output by primary hepatocytes. J Clin Endocrinol Metab 90 (2): 1055-1060.
18. Gauna C, van de Zande B, van Kerkwijk A, Themmen AP, Van Der Lely AJ, Delhanty PJ,. 2007. Unacylated ghrelin is not a functional antagonist but a full agonist of the type 1a growth hormone secretagogue receptor (GHS-R). Mol Cell Endocrinol 274 (1-2): 30-34.
19. Giovambattista A, Gaillard RC, Spinedi E,. 2008. Ghrelin gene-related peptides modulate rat white adiposity. Vitam Horm 77: 171-205.
20. Gnanapavan S, Kola B, Bustin SA, Morris DG, McGee P, Fairclough P, Bhattacharya S, Carpenter R, Grossman AB, Korbonits M,. 2002. The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans. J Clin Endocrinol Metab 87 (6): 2988.
21. Gourcerol G, Coskun T, Craft LS, Mayer JP, Heiman ML, Wang L, Million M, St-Pierre DH, Taché Y,. 2007. Preproghrelin-derived peptide, obestatin, fails to influence food intake in lean or obese rodents. Obesity (Silver Spring) 15 (11): 2643-2652.
22. Gutierrez-Grobe Y, Villalobos-Blasquez I, Sánchez-Lara K, Villa AR, Ponciano-Rodríguez G, Ramos MH, Chavez-Tapia NC, Uribe M, Méndez-Sánchez N,. 2010. High ghrelin and obestatin levels and low risk of developing fatty liver. Ann Hepatol 9 (1): 52-57.
23. Holst B, Holliday ND, Bach A, Elling CE, Cox HM, Schwartz TW,. 2004. Common structural basis for constitutive activity of the ghrelin receptor family. J Biol Chem 279 (51): 53806-53817.
24. Kim MS, Yoon CY, Jang PG, Park YJ, Shin CS, Park HS, Ryu JW, Pak YK, Park JY, Lee KU, Kim SY, Lee HK, Kim YB, Park KS,. 2004. The mitogenic and antiapoptotic actions of ghrelin in 3T3-L1 adipocytes. Mol Endocrinol 18 (9): 2291-2301.
25. Kirchner H, Gutierrez JA, Solenberg PJ, Pfluger PT, Czyzyk TA, Willency JA, Schürmann A, Joost HG, Jandacek RJ, Hale JE, Heiman ML, Tschöp MH,. 2009. GOAT links dietary lipids with the endocrine control of energy balance. Nat Med 15 (7): 741-745.
26. Kojima M, Kangawa K,. 2005. Ghrelin: Structure and function. Physiol Rev 85: 495-522.
27. Kos K, Harte AL, O'Hare PJ, Kumar S, McTernan PG,. 2009. Ghrelin and the differential regulation of des-acyl (DSG) and oct-anoyl ghrelin (OTG) in human adipose tissue (AT). Clin Endocrinol (Oxf) 70 (3): 383-389.
28. Liu J, Lin H, Cheng P, Hu X, Lu H,. 2009. Effects of ghrelin on the proliferation and differentiation of 3T3-L1 preadipocytes. J Huazhong Univ Sci Technol Med Sci 29 (2): 227-230.
29. MacLaren R, Cui W, Simard S, Cianflone K,. 2008. Influence of obesity and insulin sensitivity on insulin signaling genes in human omental and subcutaneous adipose tissue. J Lipid Res 49 (2): 308-323.
30. Merrifield RB, 1963. Solid phase peptide synthesis. I. The synthesis of a tetrapeptide. J Am Chem Soc 85: 2149.
31. Muccioli G, Pons N, Ghà C, Catapano F, Granata R, Ghigo E,. 2004. Ghrelin and des-acyl ghrelin both inhibit isoproterenol-induced lipolysis in rat adipocytes via a non-type 1a growth hormone secretagogue receptor. Eur J Pharmacol 498 (1-3): 27-35.
32. Muccioli G, Baragli A, Granata R, Papotti M, Ghigo E,. 2007. Heterogeneity of ghrelin/growth hormone secretagogue receptors. Toward the understanding of the molecular identity of novel ghrelin/GHS receptors. Neuroendocrinology 86 (3): 147-1164.
33. Nakahara T, Harada T, Yasuhara D, Shimada N, Amitani H, Sakoguchi T, Kamiji MM, Asakawa A, Inui A,. 2008. Plasma obestatin concentrations are negatively correlated with body mass index, insulin resistance index, and plasma leptin concentrations in obesity and anorexia nervosa. Biol Psychiatry 64 (3): 252-255.
34. Neary NM, Druce MR, Small CJ, Bloom SR,. 2006. Acylated ghrelin stimulates food intake in the fed and fasted states but desacylated ghrelin has no effect. Gut 55 (1): 135.
35. Patel AD, Stanley SA, Murphy KG, Frost GS, Gardiner JV, Kent AS, White NE, Ghatei MA, Bloom SR,. 2006. Ghrelin stimulates insulin-induced glucose uptake in adipocytes. Regul Pept 134 (1): 17-122.
36. Pemberton CJ, Richards AM,. 2008. Biochemistry of ghrelin precursor peptides. Vitam Horm 77: 13-30.
37. Pfluger PT, Kirchner H, Günnel S, Schrott B, Perez-Tilve D, Fu S, Benoit SC, Horvath T, Joost HG, Wortley KE, Sleeman MW, Tschöp MH,. 2008. Simultaneous deletion of ghrelin and its receptor increases motor activity and energy expenditure. Am J Physiol Gastrointest Liver Physiol 294 (3): G610-G618.
38. Rodríguez A, Gõmez-Ambrosi J, Catalán V, Gil MJ, Becerril S, Sáinz N, Silva C, Salvador J, Colina I, Frühbeck G,. 2009. Acylated and desacyl ghrelin stimulate lipid accumulation in human visceral adipocytes. Int J Obes (Lond) 33 (5): 541-552.
39. Sato M, Nakahara K, Goto S, Kaiya H, Miyazato M, Date Y, Nakazato M, Kangawa K, Murakami N,. 2006. Effects of ghrelin and des-acyl ghrelin on neurogenesis of the rat fetal spinal cord. Biochem Biophys Res Commun 350 (3): 598-603.
40. Soares JB, Leite-Moreira AF,. 2008. Ghrelin, des-acyl ghrelin and obestatin: Three pieces of the same puzzle. Peptides 29 (7): 1255-1270.
41. Sun Q, Zang WJ, Chen C,. 2010. Growth hormone secretagogues reduce transient outward K+ current via phospholipase C/protein kinase C signaling pathway in rat ventricular myocytes. Endocrinology 151 (3): 1228-1235.
42. Tang SQ, Jiang QY, Zhang YL, Zhu XT, Shu G, Gao P, Feng DY, Wang XQ, Dong XY,. 2008. Obestatin: Its physicochemical characteristics and physiological functions. Peptides 29 (4): 639-645.
43. Taub DD,. 2008. Novel connections between the neuroendocrine and immune systems: The ghrelin immunoregulatory network. Vitam Horm 77: 325-346.
44. Tremblay F, Perreault M, Klaman LD, Tobin JF, Smith E, Gimeno RE,. 2007. Normal food intake and body weight in mice lacking the G protein-coupled receptor GPR39. Endocrinology 148 (2): 501-506.
45. Wen Y, Wang H, MacLaren R, Wu J, Lu H, Cianflone K,. 2008. Palmitate and oleate induction of acylation stimulating protein resistance in 3T3-L1 adipocytes and preadipocytes. J Cell Biochem 104 (2): 391-401.
46. Yano Y, Toshinai K, Inokuchi T, Kangawa K, Shimada K, Kario K, Nakazato M,. 2009. Plasma des-acyl ghrelin, but not plasma HMW adiponectin, is a useful cardiometabolic marker for predicting atherosclerosis in elderly hypertensive patients. Atherosclerosis 204 (2): 590-594.
47. Zhang W, Zhao L, Lin TR, Chai B, Fan Y, Gantz I, Mulholland MW,. 2004. Inhibition of adipogenesis by ghrelin. Mol Biol Cell 15 (5): 2484-2491.
48. Zhang W, Chai B, Li JY, Wang H, Mulholland MW,. 2008. Effect of des-acyl ghrelin on adiposity and glucose metabolism. Endocrinology 149 (9): 4710-4716.
49. Zwirska-Korczala K, Adamczyk-Sowa M, Sowa P, Pilc K, Suchanek R, Pierzchala K, Namyslowski G, Misiolek M, Sodowski K, Kato I, Kuwahara A, Zabielski R,. 2007. Role of leptin, ghrelin, angiotensin II and orexins in 3T3 L1 preadipocyte cells proliferation and oxidative metabolism. J Physiol Pharmacol 58 (Suppl 1): 53-64.