One-source peptide/phosphopeptide standards for accurate phosphorylation degree determination

Proteomics

Volumn 11, Issue 3, 2011, Pages 490-494

  Hahn, Bettina ^a   Böhm, Martin ^a   Raia, Valentina ^b   Zinn, Nico ^a   Möller, Peter ^c   Klingmüller, Ursula ^b   Lehmann, Wolf Dieter ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b GERMAN CANCER RESEARCH CENTER   (Germany)

^c UNIVERSITY HOSPITAL ULM   (Germany)

Author keywords

One source peptide phosphopeptide standards; Phosphorylation degree; Relative quantification; Stable isotope labeling; STAT; Technology

Indexed keywords

PHOSPHOPEPTIDE; STAT5A PROTEIN; STAT5B PROTEIN; STAT6 PROTEIN;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; IMMUNOPRECIPITATION; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; OXIDATION; PEPTIDE ANALYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEPHOSPHORYLATION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

CHROMATOGRAPHY, LIQUID; HODGKIN DISEASE; HUMANS; PHOSPHOPEPTIDES; PHOSPHORYLATION; PROTEOMICS; REFERENCE STANDARDS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; STAT5 TRANSCRIPTION FACTOR; STAT6 TRANSCRIPTION FACTOR; TUMOR CELLS, CULTURED; TUMOR SUPPRESSOR PROTEINS;

EID: 79251527894     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201000569     Document Type: Article

References (20)

1. Marks, F., Klingmüller, U., Müller-Decker, K., Cellular Signal Processing: An Introduction to the Molecular Mechanisms of Signal Transduction. Garland Science, New York 2008.
2. Preisinger, C., von Kriegsheim, A., Matallanas, D., Kolch, W., Proteomics and phosphoproteomics for the mapping of cellular signalling networks. Proteomics 2008, 8, 4402-4415.
3. Aebersold, R., Rist, B., Gygi, S. P., Quantitative proteome analysis: methods and applications. Ann. N. Y. Acad. Sci. 2000, 919, 33-47
4. Heck, A. J., Krijgsveld, J., Mass spectrometry-based quantitative proteomics. Expert Rev. Proteomics 2004, 1, 317-326.
5. Ong, S. E., Mann, M., Mass spectrometry-based proteomics turns quantitative. Nat. Chem. Biol. 2005, 1, 252-262.
6. Bantscheff, M., Schirle, M., Sweetman, G., Rick, J., Kuster, B., Quantitative mass spectrometry in proteomics: a critical review. Anal. Bioanal. Chem. 2007, 389, 1017-1031.
7. Tedford, N. C., Hall, A. B., Graham, J. R., Murphy, C. E. et al., Quantitative analysis of cell signaling and drug action via mass spectrometry-based systems level phosphoproteomics. Proteomics 2009, 9, 1469-1487.
8. Olsen, J. V., Blagoev, B., Gnad, F., Macek, B. et al., Global, in vivo and site-specific phosphorylation dynamics in signaling networks. Cell 2006, 127, 635-648.
9. Seidler, J., Adal, M., Kubler, D., Bossemeyer, D., Lehmann, W. D., Analysis of autophosphorylation sites in the recombinant catalytic subunit alpha of cAMP-dependent kinase by nano-UPLC-ESI-MS/MS. Anal. Bioanal. Chem. 2009, 395, 1713-1720.
10. Schilling, M., Maiwald, T., Hengl, S., Winter, D. et al., Theoretical and experimental analysis links isoform-specific ERK signalling to cell fate decisions. Mol. Syst. Biol. 2009, 5, 334.
11. Winter, D., Seidler, J., Ziv, Y., Shiloh, Y., Lehmann, W. D., Citrate boosts the performance of phosphopeptide analysis by UPLC-ESI-MS/MS. J. Proteome Res. 2009, 8, 418-424.
12. Gropengiesser, J., Varadarajan, B. T., Stephanowitz, H., Krause, E., The relative influence of phosphorylation and methylation on responsiveness of peptides to MALDI and ESI mass spectrometry. J. Mass Spectrom. 2009, 44, 821-831.
13. Steen, H., Jebanathirajah, J. A., Springer, M., Kirschner, M. W., Stable isotope-free relative and absolute quantitation of protein phosphorylation stoichiometry by MS. Proc. Natl. Acad. Sci. USA 2005, 102, 3948-3953.
14. Zhang, X., Jin, Q. K., Carr, S. A., Annan, R. S., N-Terminal peptide labeling strategy for incorporation of isotopic tags: a method for the determination of site-specific absolute phosphorylation stoichiometry. Rapid Commun. Mass Spectrom. 2002, 16, 2325-2332.
15. Hegeman, A. D., Harms, A. C., Sussman, M. R., Bunner, A. E., Harper, J. F., An isotope labeling strategy for quantifying the degree of phosphorylation at multiple sites in proteins. J. Am. Soc. Mass Spectrom. 2004, 15, 647-653.
16. Atrih, A., Turnock, D., Sellar, G., Thompson, A. et al., Stoichiometric quantification of Akt phosphorylation using LC-MS/MS. J. Proteome Res. 2010, 9, 743-751.
17. Zinn, N., Hahn, B., Pipkorn, R., Schwarzer, D., Lehmann, W. D., Phosphorus-based absolutely quantified standard peptides for quantitative proteomics. J. Proteome Res. 2009, 8, 4870-4875.
18. Möller, P., Brüderlein, S., Sträter, J., Leithäuser, F. et al., MedB-1, a human tumor cell line derived from a primary mediastinal large B-cell lymphoma. Int. J. Cancer 2001, 92, 348-353.
19. Shevchenko, A., Tomas, H., Havlis, J., Olsen, J. V., Mann, M., In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat. Protoc. 2006, 1, 2856-2860.
20. Winter, D., Kugelstadt, D., Seidler, J., Kappes, B., Lehmann, W. D., Protein phosphorylation influences proteolytic cleavage and kinase substrate properties exemplified by analysis of in vitro phosphorylated Plasmodium falciparum glideosome-associated protein 45 by nano-ultra performance liquid chromatography-tandem mass spectrometry. Anal. Biochem. 2009, 393, 41-47.