Analysis of autophosphorylation sites in the recombinant catalytic subunit alpha of cAMP-dependent kinase by nano-UPLC-ESI-MS/MS

Analytical and Bioanalytical Chemistry

Volumn 395, Issue 6, 2009, Pages 1713-1720

  Seidler, Joerg ^a   Adal, Melaku ^a   Kübler, Dieter ^a   Bossemeyer, Dirk ^a   Lehmann, Wolf D ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

Electrospray; LC MS; Mass spectrometry; Phosphorylation degree; Protein kinase A; Protein phosphorylation

Indexed keywords

1D GEL ELECTROPHORESIS; AUTOPHOSPHORYLATION; CATALYTIC SUBUNITS; CHARGE STATE; CONSENSUS MOTIF; CYCLIC ADENOSINE; ELECTROSPRAY; ESI-MS/MS; IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHIES; INGEL DIGESTION; LC-MS; NATIVE PROTEINS; PHOSPHOPEPTIDES; PHOSPHORYLATION SITES; PROTEIN KINASE A; PROTEIN PHOSPHORYLATION; SIGNAL INTENSITIES; SIGNAL INTENSITY MEASUREMENTS; SITE-SPECIFIC; ULTRAPERFORMANCE LIQUID CHROMATOGRAPHY; WILD TYPES;

AFFINITY CHROMATOGRAPHY; AMINES; CHROMATOGRAPHIC ANALYSIS; ELECTROPHORESIS; FETAL MONITORING; GELATION; GELS; ION CHROMATOGRAPHY; MASS SPECTROMETERS; MASS SPECTROMETRY; METAL IONS; TISSUE;

PHOSPHORYLATION;

CYCLIC AMP DEPENDENT PROTEIN KINASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVE SITE; EVALUATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METHODOLOGY; MOLECULAR GENETICS; PHOSPHORYLATION; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; CATALYTIC DOMAIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYCLIC AMP-DEPENDENT PROTEIN KINASES; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY;

EID: 70449574788     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-009-2932-4     Document Type: Article

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