Protein phosphorylation influences proteolytic cleavage and kinase substrate properties exemplified by analysis of in vitro phosphorylated Plasmodium falciparum glideosome-associated protein 45 by nano-ultra performance liquid chromatography-tandem mass spectrometry

Analytical Biochemistry

Volumn 393, Issue 1, 2009, Pages 41-47

  Winter, Dominic ^a   Kugelstadt, Dominik ^b   Seidler, Joerg ^a   Kappes, Barbara ^b   Lehmann, Wolf D ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

Author keywords

Hierarchical phosphorylation; Interference of phosphorylation with proteolysis; Mass spectrometry; Mutually exclusive phosphorylation; PfCDPK1; PfGAP45; Phosphorylation

Indexed keywords

AFFINITY CHROMATOGRAPHY; DRUG PRODUCTS; ELECTROSPRAY IONIZATION; ENZYMES; GALLIUM COMPOUNDS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PROTEOLYSIS;

CALCIUM DEPENDENT PROTEIN KINASE; HIERARCHICAL PHOSPHORYLATION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; PFCDPK1; PFGAP45; PHOSPHOPEPTIDE ENRICHMENT; PROTEIN PHOSPHORYLATION; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

PHOSPHORYLATION;

CHYMOTRYPSIN; ELASTASE; GLIDEOSOME ASSOCIATED PROTEIN 45; NANOPARTICLE; PHOSPHOPEPTIDE; PROTEIN ASPN; PROTEIN KINASE; PROTEINASE; SERINE; THREONINE; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IN VITRO STUDY; LIQUID CHROMATOGRAPHY; NONHUMAN; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN PHOSPHORYLATION; TANDEM MASS SPECTROMETRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

PLASMODIUM FALCIPARUM;

EID: 67651004664     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.06.022     Document Type: Article

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