Expression, purification and analysis of the activity of enzymes from the pentose phosphate pathway

Protein Expression and Purification

Volumn 76, Issue 2, 2011, Pages 229-237

  Arthur, Patrick K ^a   Alvarado, Luigi J ^a   Dayie, T Kwaku ^a  

^a UNIVERSITY OF MARYLAND   (United States)

Author keywords

Affinity chromatography; CTP synthetase; E. coli BL21(AI) strains; His tagged recombinant E. coli ribokinase; Human PRPP synthetase; Phosphoribosyltransferases

Indexed keywords

CYTIDINE TRIPHOSPHATE SYNTHASE; GLYCOSYLTRANSFERASE; HISTIDINE; HYBRID PROTEIN; LIGASE; NICKEL; PRPS1 PROTEIN, HUMAN; RIBOSEPHOSPHATE PYROPHOSPHOKINASE;

AFFINITY CHROMATOGRAPHY; ARTICLE; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; NUCLEAR MAGNETIC RESONANCE; PENTOSE PHOSPHATE CYCLE; POLYACRYLAMIDE GEL ELECTROPHORESIS;

CARBON-NITROGEN LIGASES; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HISTIDINE; HUMANS; NICKEL; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PENTOSE PHOSPHATE PATHWAY; PENTOSYLTRANSFERASES; RECOMBINANT FUSION PROTEINS; RIBOSE-PHOSPHATE PYROPHOSPHOKINASE;

ESCHERICHIA COLI;

EID: 79151483264     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.11.008     Document Type: Article

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