Heterologous expression, purification and characterization of heterodimeric monellin

Protein Expression and Purification

Volumn 76, Issue 2, 2011, Pages 248-253

  Aghera, Nilesh ^a   Udgaonkar, Jayant B ^a  

^a TATA INSTITUTE OF FUNDAMENTAL RESEARCH   (India)

Author keywords

Co expression; Monellin; pETDUET; Protein purification

Indexed keywords

MONELLIN PROTEIN, DIOSCOREOPHYLLUM CUMMINSII; PROTEIN SUBUNIT; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN FOLDING; SPECTROFLUOROMETRY;

CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; PLANT PROTEINS; PROTEIN FOLDING; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

DIOSCOREOPHYLLUM CUMMINSII; ESCHERICHIA COLI;

EID: 79151470987     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.11.002     Document Type: Article

References (51)

1. J.A. Morris, and R.H. Cagan Purification of monellin, the sweet principle of Dioscoreophyllum cumminsii Biochimica et Biophysica Acta 261 1972 114 122
2. R.H. Cagan Chemostimulatory protein: a new type of taste stimulus Science (New York) 181 1973 32 35
3. I. G. E, M.A.Y. Joann F, Serendipity Berries-Source of a New Intense Sweetener, Journal of Food Science, 1969, pp. 408-411.
4. J.A. Morris, R. Martenson, G. Deibler, and R.H. Cagan Characterization of monellin, a protein that tastes sweet The Journal of Biological Chemistry 248 1973 534 539
5. H. van der Wel Isolation and characterization of the sweet principle from Dioscoreophyllum cumminsii (stapf) diels FEBS Letters 21 1972 88 90
6. C. Ogata, M. Hatada, G. Tomlinson, W.C. Shin, and S.H. Kim Crystal structure of the intensely sweet protein monellin Nature 328 1987 739 742
7. J.R. Somoza, F. Jiang, L. Tong, C.H. Kang, J.M. Cho, and S.H. Kim Two crystal structures of a potently sweet protein. Natural monellin at 2.75 A resolution and single-chain monellin at 1.7 A resolution Journal of Molecular Biology 234 1993 390 404
8. A.G. Murzin Sweet-tasting protein monellin is related to the cystatin family of thiol proteinase inhibitors Journal of Molecular Biology 230 1993 689 694
9. S.H. Kim, C.H. Kang, R. Kim, J.M. Cho, Y.B. Lee, and T.K. Lee Redesigning a sweet protein: increased stability and renaturability Protein Engineering 2 1989 571 575
10. M. Kohmura, N. Nio, and Y. Ariyoshi Highly probable active site of the sweet protein monellin Bioscience, Biotechnology, and Biochemistry 56 1992 1937 1942
11. J.A. Morris, and R.H. Cagan Enzymatic proteolysis of monellin. Absence of sweet peptides Journal of Agricultural and Food Chemistry 24 1976 1075 1077
12. 1H NMR investigation of pMNEI FEBS Letters 310 1992 27 30
13. R. Spadaccini, F. Trabucco, G. Saviano, D. Picone, O. Crescenzi, T. Tancredi, and P.A. Temussi The mechanism of interaction of sweet proteins with the T1R2-T1R3 receptor: evidence from the solution structure of G16A-MNEI Journal of Molecular Biology 328 2003 683 692
14. T. Tancredi, A. Pastore, S. Salvadori, V. Esposito, and P.A. Temussi Interaction of sweet proteins with their receptor. A conformational study of peptides corresponding to loops of brazzein, monellin and thaumatin European Journal of Biochemistry/FEBS 271 2004 2231 2240
15. P.A. Temussi Why are sweet proteins sweet? Interaction of brazzein, monellin and thaumatin with the T1R2-T1R3 receptor FEBS Letters 526 2002 1 4
16. G. Hellekant, Y. Ninomiya, and V. Danilova Taste in chimpanzees II: single chorda tympani fibers Physiology and Behavior 61 1997 829 841
17. C.R. Plata-Salaman, T.R. Scott, and V.L. Smith-Swintosky Gustatory neural coding in the monkey cortex: the quality of sweetness Journal of Neurophysiology 69 1993 482 493
18. A. Schilling, V. Danilova, and G. Hellekant Behavioral study in the gray mouse lemur (Microcebusmurinus) using compounds considered sweet by humans American Journal of Primatology 62 2004 43 48
19. K. Tonosaki, K. Miwa, and F. Kanemura Gustatory receptor cell responses to the sweeteners, monellin and thaumatin Brain Research 748 1997 234 236
20. R. Spadaccini, O. Crescenzi, T. Tancredi, N. De Casamassimi, G. Saviano, R. Scognamiglio, A. Di Donato, and P.A. Temussi Solution structure of a sweet protein: NMR study of MNEI a single chain monellin Journal of Molecular Biology 305 2001 505 514
21. S.K. Jha, D. Dhar, G. Krishnamoorthy, and J.B. Udgaonkar Continuous dissolution of structure during the unfolding of a small protein Proceedings of the National Academy of Sciences of the United States of America 106 2009 11113 11118
22. S.K. Jha, and J.B. Udgaonkar Direct evidence for a dry molten globule intermediate during the unfolding of a small protein Proceedings of the National Academy of Sciences of the United States of America 106 2009 12289 12294
23. T. Kimura, A. Maeda, S. Nishiguchi, K. Ishimori, I. Morishima, T. Konno, Y. Goto, and S. Takahashi Dehydration of main-chain amides in the final folding step of single-chain monellin revealed by time-resolved infrared spectroscopy Proceedings of the National Academy of Sciences of the United States of America 105 2008 13391 13396
24. T. Kimura, T. Uzawa, K. Ishimori, I. Morishima, S. Takahashi, T. Konno, S. Akiyama, and T. Fujisawa Specific collapse followed by slow hydrogen-bond formation of beta-sheet in the folding of single-chain monellin Proceedings of the National Academy of Sciences of the United States of America 102 2005 2748 2753
25. A.K. Patra, and J.B. Udgaonkar Characterization of the folding and unfolding reactions of single-chain monellin: evidence for multiple intermediates and competing pathways Biochemistry 46 2007 11727 11743
26. Y.H. Sung, H.D. Hong, C. Cheong, J.H. Kim, J.M. Cho, Y.R. Kim, and W. Lee Folding and stability of sweet protein single-chain monellin. An insight to protein engineering The Journal of Biological Chemistry 276 2001 44229 44238
27. J. Peon, S.K. Pal, and A.H. Zewail Hydration at the surface of the protein monellin: dynamics with femtosecond resolution Proceedings of the National Academy of Sciences of the United States of America 99 2002 10964 10969
28. J. Xu, D. Toptygin, K.J. Graver, R.A. Albertini, R.S. Savtchenko, N.D. Meadow, S. Roseman, P.R. Callis, L. Brand, and J.R. Knutson Ultrafast fluorescence dynamics of tryptophan in the proteins monellin and IIAGlc Journal of the American Chemical Society 128 2006 1214 1221
29. A.K. Patra, and J.B. Udgaonkar GroEL can unfold late intermediates populated on the folding pathways of monellin Journal of Molecular Biology 389 2009 759 775
30. V. Esposito, F. Guglielmi, S.R. Martin, K. Pauwels, A. Pastore, R. Piccoli, and P.A. Temussi Aggregation mechanisms of cystatins: a comparative study of monellin and oryzacystatin Biochemistry 49 2010 2805 2810
31. P. Fan, C. Bracken, and J. Baum Structural characterization of monellin in the alcohol-denatured state by NMR: evidence for beta-sheet to alpha-helix conversion Biochemistry 32 1993 1573 1582
32. T. Konno Multistep nucleus formation and a separate subunit contribution of the amyloid genesis of heat-denatured monellin Protein Science 10 2001 2093 2101
33. T. Konno, K. Murata, and K. Nagayama Amyloid-like aggregates of a plant protein: a case of a sweet-tasting protein monellin FEBS Letters 454 1999 122 126
34. W.F. Xue, J. Carey, and S. Linse Multi-method global analysis of thermodynamics and kinetics in reconstitution of monellin Proteins 57 2004 586 595
35. W.F. Xue, O. Szczepankiewicz, M.C. Bauer, E. Thulin, and S. Linse Intra- versus intermolecular interactions in monellin: contribution of surface charges to protein assembly Journal of Molecular Biology 358 2006 1244 1255
36. M. Kohmura, N. Nio, and Y. Ariyoshi Complete amino acid sequence of the sweet protein monellin Agricultural and Biological Chemistry 54 1990 2219 2224
37. I. Faus Recent developments in the characterization and biotechnological production of sweet-tasting proteins Applied Microbiology and Biotechnology 53 2000 145 151
38. T. Masuda, and N. Kitabatake Developments in biotechnological production of sweet proteins Journal of Bioscience and Bioengineering 102 2006 375 389
39. W.F. Xue, O. Szczepankiewicz, E. Thulin, S. Linse, and J. Carey Role of protein surface charge in monellin sweetness Biochimica et Biophysica Acta 1794 2009 410 420
40. K. Ponniah, T.S. Loo, P.J. Edwards, S.M. Pascal, G.B. Jameson, and G.E. Norris The production of soluble and correctly folded recombinant bovine beta-lactoglobulin variants A and B in Escherichia coli for NMR studies Protein Expression and Purification 70 2010 283 289
41. X. Wang, Z. Zheng, P. Dou, J. Qin, X. Wang, C. Ma, H. Tang, P. Xu, Cloning, expression, purification, and activity assay of proteins related to D-lactic acid formation in Lactobacillus rhamnosus, Applied Microbiology and Biotechnology 87 (2010) 2117-2123.
42. H. Schagger, and G. von Jagow Tricine-sodium dodecyl sulfate- polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa Analytical Biochemistry 166 1987 368 379
43. H. Schagger Tricine-SDS-PAGE Nature Protocols 1 2006 16 22
44. P.K. Smith, R.I. Krohn, G.T. Hermanson, A.K. Mallia, F.H. Gartner, M.D. Provenzano, E.K. Fujimoto, N.M. Goeke, B.J. Olson, and D.C. Klenk Measurement of protein using bicinchoninic acid Analytical Biochemistry 150 1985 76 85
45. Z. Chen, H. Cai, F. Lu, and L. Du High-level expression of a synthetic gene encoding a sweet protein, monellin, in Escherichia coli Biotechnology Letters 27 2005 1745 1749
46. I.H. Kim, and K.J. Lim Large-scale purification of recombinant monellin from yeast Journal of Fermentation and Bioengineering 82 1996 180 182
47. K. Kondo, Y. Miura, H. Sone, K. Kobayashi, and H. Iijima High-level expression of a sweet protein, monellin, in the food yeast Candida utilis Nature Biotechnology 15 1997 453 457
48. L. Penarrubia, R. Kim, J. Giovannoni, S.-H. Kim, and R.L. Fischer Production of the sweet protein monellin in transgenic plants Nature Biotechnology 10 1992 561 564
49. K. Roh, K.-S. Shin, Y.-H. Lee, S.-C. Seo, H.-G. Park, H. Daniell, and S.-B. Lee Accumulation of sweet protein monellin is regulated by the psbA 5′UTR in tobacco chloroplasts Journal of Plant Biology 49 2006 34 43
50. M. Kohmura, N. Nio, and Y. Ariyoshi Solid-phase synthesis of crystalline [Ser41] B-chain monellin, an analogue of the sweet protein monellin Agricultural and Biological Chemistry 55 1991 1831 1838
51. M. Ota, and Y. Ariyoshi Solid-phase synthesis of single-chain monellin, a sweet protein Bioscience, Biotechnology, and Biochemistry 62 1998 2043 2045