Solid-phase synthesis of single-chain monellin, a sweet protein

Bioscience, Biotechnology and Biochemistry

Volumn 62, Issue 10, 1998, Pages 2043-2045

  Ota, Masafumi ^a   Ariyoshi, Yasuo ^a  

^a AJINOMOTO CO INC   (Japan)

Author keywords

Protein synthesis; Single chain monellin; Solid phase synthesis; Sweet protein; Sweetener

Indexed keywords

MONELLIN PROTEIN, DIOSCOREOPHYLLUM CUMMINSII; SWEETENING AGENT; VEGETABLE PROTEIN;

ARTICLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SYNTHESIS;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; PLANT PROTEINS; SWEETENING AGENTS;

EID: 0032174633     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.62.2043     Document Type: Article

References (11)

1. Morris, J. A. and Cagan, R. H., Purification of monellin, the sweet principle of Dioscoreophyllum cumminsii. Biochem. Biophys. Acta, 261, 114-122 (1972).
2. van der Wei, H., Isolation and characterization of the sweet principle from Dioscoreophyllum cumminsii (Stapf) Diels. FEBS Lett., 21, 88-90 (1972).
3. Kohmura, M., Nio, N., and Ariyoshi, Y., Complete amino acid sequence of the sweet protein monellin. Agric. Biol. Chem., 54, 2219-2224 (1990).
4. Kohmura, M., Nio, N., and Ariyoshi, Y., Solid-phase synthesis of crystalline monellin, a sweet protein. Agric. Biol. Chem., 55, 539-545 (1991).
5. Kim, S.-H., Kang, C.-H., Kim, R., Cho, J. M., Lee, Y.-B., and Lee, T.-K., Redesigning a sweet protein: increased stability and renaturability. Protein Engineering, 2, 571-575 (1989).
6. Kondo, K., Miura, Y., Sone, H., Kobayashi, K., and Iijima, H., High-level expression of a sweet protein, monellin, in the food yeast Candida utilis. Nature Biotechnology, 15, 453-457 (1997).
7. Barlos, K., Gatos, D., and Schäfer, W., Synthesis of prothymosin a (ProTa)—a protein consisting of 109 amino acid residues. Angew. Chem. Int. Ed. Engl. 30, 590-593 (1991).
8. Barlos, K., Chatzi, O., Gatos, D., and Stavropoulos, G., 2-Chlo-rotrityl chloride resin. Int. J. Peptide Protein Res., 37, 513-520 (1991).
9. Meienhofer, J., Waki, M., Heimer, E. P., Lambros, T. J., Makofske, R. C., and Chang, C. D., Solid phase synthesis without repetitive acidolysis. Int. J. Peptide Protein Res., 13, 35-42 (1979).
10. Zhang, L., Goldammer, C., Henkel, B., Zühl, F., Panhaus, G., Jung, G., and Bayer, E., “Magic mixture”, a powerful solvent system for solid-phase synthesis of “difficult sequences”. In “Solid phase synthesis 1994”, eds. Epton, R., Mayflower Worldwide Ltd., Birmingham, pp. 711-716 (1994).
11. King, D. S., Fields, C. G., and Fields, G. B., A cleavage method which minimizes side reactions following Fmoc solid phase peptide synthesis. Int. J. Peptide Protein Res., 36, 255-266 (1990).