Chrysin abrogates early hepatocarcinogenesis and induces apoptosis in N-nitrosodiethylamine-induced preneoplastic nodules in rats

Toxicology and Applied Pharmacology

Volumn 251, Issue 1, 2011, Pages 85-94

  Khan, Mahaboob S ^a,b   Devaraj, Halagowder ^c   Devaraj, Niranjali ^a  

^a UNIVERSITY OF MADRAS   (India)

^b PANJAB UNIVERSITY   (India)

^c Unit of Biochemistry   (India)

Author keywords

Apoptosis; Chemoprevention; Chrysin; Diethylnitrosamine; Experimental hepatocarcinogenesis; Neoplastic nodules

Indexed keywords

ALANINE AMINOTRANSFERASE; ALKALINE PHOSPHATASE; ASPARTATE AMINOTRANSFERASE; BETA ARRESTIN 2; C80105; CASPASE 3; CHRYSIN; CYCLOOXYGENASE 2; DIETHYLNITROSAMINE; GAMMA GLUTAMYLTRANSFERASE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; LACTATE DEHYDROGENASE; MESSENGER RNA; PROTEIN BCL XL; PROTEIN P53; SYNAPTOTAGMIN I; UNCLASSIFIED DRUG;

ALANINE AMINOTRANSFERASE BLOOD LEVEL; ALKALINE PHOSPHATASE BLOOD LEVEL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; APOPTOSIS; ARTICLE; ASPARTATE AMINOTRANSFERASE BLOOD LEVEL; CANCER GROWTH; CELL PROLIFERATION; CONTROLLED STUDY; DIET SUPPLEMENTATION; ENZYME ACTIVITY; GAMMA GLUTAMYL TRANSFERASE BLOOD LEVEL; IN VITRO STUDY; LACTATE DEHYDROGENASE BLOOD LEVEL; LIVER CARCINOGENESIS; MALE; NONHUMAN; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RAT; REAL TIME POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ANIMALS; ANTICARCINOGENIC AGENTS; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; ARRESTINS; BIOLOGICAL MARKERS; BODY WEIGHT; CELL CYCLE PROTEINS; CELL PROLIFERATION; CELL TRANSFORMATION, NEOPLASTIC; CYCLOOXYGENASE 2; DIETHYLNITROSAMINE; FLAVONOIDS; GLUTATHIONE S-TRANSFERASE PI; LIVER; LIVER NEOPLASMS, EXPERIMENTAL; MALE; ORGAN SIZE; PRECANCEROUS CONDITIONS; RATS; RATS, WISTAR; TIME FACTORS; TRANSCRIPTION FACTOR RELA; TUMOR SUPPRESSOR PROTEIN P53;

RATTUS; RATTUS NORVEGICUS;

EID: 78751627519     PISSN: 0041008X     EISSN: 10960333     Source Type: Journal    
DOI: 10.1016/j.taap.2010.12.004     Document Type: Article

References (51)

1. Ahn S., Kim J., Hara M.R., Ren X.R., Lefkowitz R.J. β-Arrestin-2 mediates anti-apoptotic signaling through regulation of BAD phosphorylation. J. Biol. Chem. 2009, 284:8855-8865.
2. Banerjee S., Ramos C.B., Aggarwal B.B. Suppression of 7,12 dimethyl-benz(a)anthracene-induced mammary carcinogenesis in rats by resveratrol: role of nuclear factor-kB, cyclooxygenase-2 and matrix metalloproteinase-9. Cancer Res. 2002, 62:4945-4954.
3. Bannasch P. Sequential cellular changes during chemical carcinogenesis. J. Cancer Res. Clin. Oncol. 1984, 108:11-22.
4. Bergmeyer H.U., Bernt E. Aminotransferases and related enzymes. Methods of Enzymatic Analysis 1974, 735-763. Academic Press, New York. 2nd ed. H.U. Bergmeyer (Ed.).
5. Bishayee A., Dhir N. Resveratrol-mediated chemoprevention of diethylnitrosamine-initiated hepatocarcinogenesis: inhibition of cell proliferation and induction of apoptosis. Chem. Biol. Interact. 2009, 179:131-144.
6. Bryja V., Gradl D., Schambony A., Arenas E., Schulte G. Beta-arrestin is a necessary component of Wnt/beta-catenin signaling in vitro and in vivo. Proc. Natl Acad. Sci. 2007, 104:6690-6695.
7. Cameron R., Kellen J., Kolin A., Malkin A., Farber E. γ-Glutamyl transferase in putative premalignant liver cell populations during hepatocarcinogenesis. Cancer Res. 1978, 38:823-829.
8. Campbell H.A., Pitot H.C., Potter V.R., Laishes B.R. Application of quantitative stereology to the evaluation of enzyme altered foci in the liver. Cancer Res. 1982, 42:465-472.
9. Cao Y., Prescott S.M. Many actions of cyclooxygenase-2 in cellular dynamics and in cancer. J. Cell. Physiol. 2002, 190:279-286.
10. Cardenas M., Marder M., Blank V.C., Roguin L.P. Antitumor activity of some natural flavonoids and synthetic derivatives on various human and murine cancer cell lines. Bioorg. Med. Chem. 2006, 14:2966-2971.
11. Carrasco-Legleu C.E., Marquez-Rosado L., Fattel-Fazenda S., Arce-Popoca E., Perez-Carreon J.I., Villa-Trevino S. Chemopreventive effect of caffeic acid phenethyl ester on promotion in a medium-term rat hepatocarcinogenesis assay. Int. J. Cancer 2004, 108:488-492.
12. Chang Y.S., Hsu Y.L., Kuo P., Kuo Y.C., Chai L., Ching C.C. Increase of Bax/Bcl-Xl ratio and arrest of cell cycle by luteolin in immortalized human hepatoma cell line. Life Sci. 2005, 76:1883-1893.
13. Devi A., Devaraj H. Induction and expression of GST-Pi foci in the liver of cyclophosphamide-administered rats. Toxicology 2006, 217:120-128.
14. Finnberg N., Stenius U., Hogberg J. Xenobiotics modulate the p53 response to DNA damage in preneoplastic enzyme-altered foci in rat liver; effects of diethylnitrosamine and phenobarbital. Toxicol. Sci. 2000, 54:95-103.
15. Fu B., Xue J., Li Z., Shi X., Jiang B.H., Fang J. Chrysin inhibits expression of hypoxia-inducible factor-1alpha through reducing hypoxia-inducible factor-1alpha stability and inhibiting its protein synthesis. Mol. Cancer Ther. 2007, 6:220-226.
16. Gao H., Sun Y., Wu Y., Luan B., Wang Y., Qu B., Pei G. Identification of beta-arrestin2 as a G protein-coupled receptor-stimulated regulator of NF-kappaB pathways. Mol. Cell 2004, 14:303-317.
17. Horner, M.J., Ries, L.A.G., Krapcho, M., Neyman, N., Aminou, R., Howlader, N., Miller, B.A., Lewis, D.R., Eisner, M.P., Stinchcomb, D.G., Edwards, B.K., 2008. SEER Cancer Statistics Review, (eds). National Cancer Institute, Bethesda, MD.
18. Jemal A., Siegel R., Ward E., Hao Y., Xu J., Murray T., Thun M.J. Cancer statistics. CA Cancer J. Clin. 2008, 58:71-96.
19. Karamitopoulou E., Cioccari L., Jakob S., Vallan C., Schaffner T., Zimmermann A., Brunner T. Active caspase 3 and DNA fragmentation as markers for apoptotic cell death in primary and metastatic liver tumors. Pathology 2007, 39:558-564.
20. Kelloff G.J., Crowell J.A., Steele V.E., Lubet R.A., Malone W.A., Boone C.W., Kopelovich L., Hawk E.T., Lieberman R., Lawrence J.A., Ali I., Viner J.L., Sigman C.C. Progress in cancer chemoprevention: development of diet-derived chemopreventive agents. J. Nutr. 2000, 130:467S-471S.
21. King J. The dehydrogenases or oxidoreductase-lactate dehydrogenase. Practical Clinical Enzymology 1965, 83-93. Nostrand Company Ltd., London. D. Van (Ed.).
22. King J. The hydrolases-acid and alkaline phosphatases. Practical Clinical Enzymology 1965, 191-208. Nostrand Company Ltd., London. D. Van (Ed.).
23. King J. The transferases - alanine and aspartate transaminases. Practical Clinical Enzymology 1965, 121-128. Nostrand Company Ltd., London. D. Van (Ed.).
24. Lan M., Pei G. β-Arrestin signaling and regulation of transcription. J. Cell Sci. 2007, 120:213-218.
25. Lapidot T., Walker M.D., Kanner J. Antioxidant and prooxidant effects of phenolics on pancreatic beta-cells in vitro. J. Agric. Food Chem. 2002, 50:7220-7225.
26. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001, 25:402-408.
27. Mandal A.K., Das S., Mitra M., Rohini N.C., Chatterjee M., Das N. Vesicular flavonoid in combating diethylnitrosamine induced hepatocarcinoma in rat model. J. Exp. Ther. Oncol. 2008, 7:123-133.
28. Moore M.A., Ariyaratne Y., Badar F., Bhurgri Y., Datta K., Mathew A., Gangadharan P., Nandakumar A., Pradhananga K.K., Talukder M.H., Yeole B.B., Sobue T. Cancer epidemiology in South Asia - past, present and future. Asian Pac. J. Cancer Prev. 2010, 2:49-66.
29. Newbeme P.M. Experimental hepatocellular carcinogenesis. Cancer Res. 1976, 36:2573-2578.
30. Plummer S.M., Kaptein A., Farro S., Howells L. Inhibition of cyclooxygenase-2 expression in colon cells by the chemopreventive agent curcumin involves inhibition of NF-kB activation via the NIK/IKK signaling complex. Oncogene 1999, 18:6013-6020.
31. Puatanachokchai R., Kishidaa H., Denda A., Murata N., Konishi Y., Vinitketkumnuen U., Nak D. Inhibitory effects of lemon grass (Cymbopogon citratus, Stapf) extract on the early phase of hepatocarcinogenesis after initiation with diethylnitrosamine in male Fischer 344 rats. Cancer Lett. 2002, 183:9-15.
32. Pushpavalli G., Kalaiarasi P., Veeramani C., Pugalendi K.V. Effect of chrysin on hepatoprotective and antioxidant status in d-galactosamine-induced hepatitis in rats. Eur. J. Pharmacol. 2010, 631:36-41.
33. Rehman M.U., Buttar Q.M., Khawaja M.I., Khawaja M.R. An impending cancer crisis in developing countries: are we ready for the challenge. Asian Pac. J. Cancer Prev. 2009, 10:719-720.
34. Rosali S.B., Rau D. Serum gamma-glutamyl transpeptidase activity in alcoholism. Clin. Chim. Acta 1972, 39:41-47.
35. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning - a Laboratory Manual 1989, Cold Spring Harbor Laboratory Press, Cold Spring Harbor. 2nd Ed.
36. Satoh K., Hatayama I. Anomalous elevation of glutathione-S transferase P-form (GST-P) in the elementary process of epigenetic initiation of chemical hepatocarcinogenesis in rats. Carcinogenesis 2002, 23:1193-1198.
37. Siess M.H., Le Bon A.M., Canivenc-Lavier M.C., Amiot M.J., Sabatier S., Aubert S.Y., Suschetet M. Flavonoids of honey and propolis: characterization and effects on hepatic drug metabolizing enzymes and benzo[a]pyrene-DNA binding in rats. J. Agric. Food Chem. 1996, 44:2297-2301.
38. Sivaramakrishnan V., Niranjali S.D. Morin regulates the expression of NF-kB-p65, COX-2 and matrix metalloproteinases in diethylnitrosamine induced rat hepatocellular carcinoma. Chem. Biol. Interact. 2009, 180:353-359.
39. Stewart H.L., Williams G., Keysser C.H., Lombard L.S., Montali R.J. Histologic typing of liver tumors of the rat. J. Natl Cancer Inst. 1980, 65:179-206.
40. Stuhr L.E., Iversen V.V., Straume O., Maehle B.O., Reed R.K. Hyperbaric oxygen alone or combined with 5-FU attenuates growth of DMBA-induced rat mammary tumors. Cancer Lett. 2004, 210:35-40.
41. Tai D.I., Chang Y.H., Huang S.N., Chen T.C., Chang K.S., Liaw Y.F. Constitutive activation of nuclear factor kappaB in hepatocellular carcinoma. Cancer 2000, 89:2274-2281.
42. Tsang T.Y., Tang W.Y., Tsang W.P., Co N.N., Kong S.K., Kwok T.T. Downregulation of hepatoma-derived growth factor activates the Bad-mediated apoptotic pathway in human cancer cells. Apoptosis 2008, 13:1135-1147.
43. Walker P.R., Kokileva L., LeBlanc J., Sikorska M. Detection of the initial stages of DNA fragmentation in apoptosis. Biotechnology 1993, 15:1032-1040.
44. Walle T., Otake Y., Brubaker J.A., Walle U.K., Halushka P.V. Disposition and metabolism of the flavonoid chrysin in normal volunteers. Br. J. Clin. Pharmacol. 2001, 51:143-146.
45. Wattenberg L.W. Chemoprevention of cancer. Cancer Res. 1985, 45:1-8.
46. Weng M.S., Ho Y.S., Lin J.K. Chrysin induces G1 phase cell cycle arrest in C6 glioma cells through inducing p21Waf1/Cip1 expression: involvement of p38 mitogen-activated protein kinase. Biochem. Pharmacol. 2005, 69:1815-1827.
47. Williams G.M. Phenotypic properties of preneoplastic rat liver lesions and applications to detection of carcinogens and tumor promoters. Toxicol. Pathol. 1982, 10:3-10.
48. Williams C.A., Harborne J.B., Newman M., Greenham J., Eagles J. Chrysin and other leaf exudate flavonoids in the genus Pelargonium. Phytochemistry 1997, 46:1349-1353.
49. Woo K.J., Jeong Y.J., Park J.W., Kwon T.K. Chrysin-induced apoptosis is mediated through caspase activation and Akt inactivation in U937 leukemia cells. Biochem. Biophys. Res. Commun. 2004, 325:1215-1222.
50. Xiaodong W., Marilyn E.M. Effects of the flavonoid chrysin on nitrofurantoin pharmacokinetics in rats: potential involvement of ABCG2. Drug Metab. Dispos. 2007, 35:268-274.
51. Zhang T., Chen X., Qu L., Wu J., Cui R., Zhao Y. Chrysin and its phosphate ester inhibit cell proliferation and induce apoptosis in Hela cells. Bioorg. Med. Chem. 2004, 12:6097-6105.