The gypsy insulator of Drosophila melanogaster, together with its binding protein suppressor of Hairy-wing, facilitate high and precise expression of transgenes in Arabidopsis thaliana

Genetics

Volumn 185, Issue 4, 2010, Pages 1141-1150

  She, Wenjing ^a   Lin, Weiqiang ^a   Zhu, Yubin ^a   Chen, Yong ^a,b   Jin, Weiyuan ^a   Yang, Yanjun ^a   Han, Ning ^a   Bian, Hongwu ^a   Zhu, Muyuan ^a   Wang, Junhui ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b WENZHOU UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; SUPPRESSOR OF HAIRY WING PROTEIN; UNCLASSIFIED DRUG;

ARABIDOPSIS; ARTICLE; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; GENE EXPRESSION; GENETIC SCREENING; GENETIC VARIABILITY; GYPSY INSULATOR; INSULATOR ELEMENT; MARKER GENE; NONHUMAN; PLANT GENE; POSITION EFFECT; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; REPORTER GENE; TRANSGENE;

ANIMALS; ARABIDOPSIS; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GLUCURONIDASE; GREEN FLUORESCENT PROTEINS; INSULATOR ELEMENTS; MICROSCOPY, FLUORESCENCE; PLANTS, GENETICALLY MODIFIED; RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; RETROELEMENTS;

ARABIDOPSIS; ARABIDOPSIS THALIANA; DROSOPHILA MELANOGASTER;

EID: 78751618244     PISSN: 00166731     EISSN: 00166731     Source Type: Journal    
DOI: 10.1534/genetics.110.117960     Document Type: Article

References (55)

1. BAROLO, S., L. A. CARVER and J. W. POSAKONY, 2000 GFP and β-galactosidase transformation vectors for promoter/enhancer analysis in Drosophila. BioTechniques 29: 726-732.
2. BECHTOLD, N., J. ELLIS and G. PELLETIER, 1993 In planta Agrobacterium-mediated gene-transfer by infiltration of adult Arabidopsis thaliana plants. Compt Rend Acad Sci III 316: 1194-1199.
3. BELL, A. C., A. G. WEST and G. FELSENFELD, 2001 Insulators and boundaries: versatile regulatory elements in the eukaryotic genome. Science 291: 447-450.
4. BLILOU, I., J. XU, M. WILDWATER, V. WILLEMSEN, I. PAPONOV et al., 2005 The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots. Nature 433: 39-44.
5. BRADFORD, M. M., 1976 A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
6. BUSHEY, A. M., E. R. DORMAN and V. G. CORCES, 2008 Chromatin insulators: regulatory mechanisms and epigenetic inheritance. Mol. Cell 32: 1-9.
7. BUTAYE, K. M., I. J. GODERIS, P. F. WOUTERS, J.M. PUES, S. L.DELAURÉ et al., 2004 Stable high-level transgene expression in Arabidopsis thaliana using gene silencing mutants and matrix attachment regions. Plant J. 39: 440-449.
8. BUTAYE, K. M. J., B. P. A. CAMMUE, S. L. DELAURÉ and M. F. C. DE BOLLE, 2005 Approaches to minimize variation of transgene expression in plants. Mol. Breed. 16: 79-91.
9. CAMPISI, L., Y. YANG, Y. YI, E. HEILIG, B. HERMAN et al., 1999 Generation of enhancer trap lines in Arabidopsis and characterization of expressionpatterns inthe inflorescence. Plant J. 17: 699-707. (Pubitemid 29178042)
10. DE BOLLE, M. F., K. M. BUTAYE, I. J. GODERIS, P. F. WOUTERS, A. JACOBS et al., 2007 The influence of matrix attachment regions on transgene expression in Arabidopsis thaliana wild type and gene silencing mutants. Plant Mol. Biol. 63: 533-543.
11. DE BUCK, S., P.WINDELS, M.D.LOOSE and A. DEPICKER, 2004 Singlecopy T-DNAs integrated at different positions in the Arabidopsis genome display uniform and comparable beta-glucuronidase accumulation levels. Cell. Mol. Life Sci. 61: 2632-2645.
12. DE BUCK, S., I. PECK, C. D. WILDE, G. MARJANAC and J. NOLF, 2007 Generation of single-copy T-DNA transformants in Arabidopsis by the CRE/loxP recombination-mediated resolution system. Plant Physiol. 145: 1171-1182.
13. DE PAEPE, A., S. DE BUCK, K. HOORELBEKE, J. NOLF, I. PECK et al., 2009 High frequency of single-copy T-DNA transformants produced by floral dip in CRE-expressing Arabidopsis plants. Plant J. 59: 517-527.
14. DHARMASIRI, N., S. DHARMASIRI and M. ESTELLE, 2005a The F-box protein TIR1 is an auxin receptor. Nature 435: 441-445.
15. DHARMASIRI, N., S. DHARMASIRI, D.WEIJERS, E. LECHNER, M. YAMADA et al., 2005b Plant development is regulated by a family of auxin receptor F box proteins. Dev. Cell 9: 109-119.
16. FORSBACH, A., D. SCHUBERT, B. LECHTENBERG, M. GILS and R. SCHMIDT, 2003 A comprehensive characterization of singlecopy T-DNA insertions in the Arabidopsis thaliana genome. Plant Mol. Biol. 52: 161-176.
17. GASZNER, M., and G. FELSENFELD, 2006 Insulators: exploiting transcriptional and epigenetic mechanisms. Nat. Rev. Genet. 7: 703-713.
18. GDULA, D. A., T. I. GERASIMOVA and V. G. CORCES, 1996 Genetic and molecular analysis of the gypsy chromatin insulator of Drosophila. Proc. Natl. Acad. Sci. USA 93: 9378-9383.
19. GELVIN, S. B., and S. I. KIM, 2007 Effect of chromatin upon Agrobacterium T-DNA integration and transgene expression. Biochim. Biophys. Acta 1769: 410-421.
20. GERASIMOVA, T. I., and V. G. CORCES, 2001 Chromatin insulators and boundaries: effects on transcription and nuclear organization. Annu. Rev. Genet. 35: 193-208.
21. GOLOVNIN, A., E. MELNICK, A. MAZUR and P. GEORGIEW, 2005 Drosophila Su(Hw) insulator can stimulate transcription of a weakened yellow promoter over a distance. Genetics 170: 1133-1142.
22. HALWEG, C., W. F. THOMPSON and S. SPIKER, 2005 The Rb7 matrix attachment region increases the likelihood and magnitude of transgene expression in tobacco cells: a flow cytometric study. Plant Cell 17: 418-429.
23. HARRISON, D. A., D. A. GDULA, R. S. COYNE and V. G. CORCES, 1993 A leucine zipper domain of the suppressor of Hairy-wing protein mediates its repressive effect on enhancer function. Genes Dev. 7: 1966-1978.
24. HENDERSON, I. R., and S. E. JACOBSEN, 2007 Epigenetic inheritance in plants. Nature 447: 418-424.
25. HOLMES-DAVIS, R., and L. COMAI, 1998 Nuclear matrix attachment regions and plant gene expression. Trends Plant Sci. 3: 91-97.
26. HUNT, L., F. LERNER and M. ZIEGLER, 2004 NAD-new roles in signaling and gene regulation in plants. New Phytol. 163: 31-44.
27. JEFFERSON, R. A., T. A. KAVANAGH and M. W. BEVAN, 1987 GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6: 3901-3907.
28. KARIMI, M., D. INZE and A. DEPICKER, 2002 GATEWAY™ vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci. 7: 193-195.
29. KELLUM, R., and P. SCHEDL, 1991 A position-effect assay for boundaries of higher order chromosomal domains. Cell 64: 941-950.
30. KEPINSKI, S., and O. LEYSER, 2005 The Arabidopsis F-box protein TIR1 is an auxin receptor. Nature 435: 446-451.
31. KIM, S. I., VEENA, S. B. GELVIN, 2007 Genome-wide analysis of Agrobacterium T-DNA integration site in Arabidopsis genome generated under non-selective conditions. Plant J. 51: 779-791.
32. KUHN, E. J., and P. K. GEYER, 2003 Genomic insulators: connecting properties to mechanism. Curr. Opin. Cell Biol. 15: 259-265.
33. LAPLAZE, L., B. PARIZOT, A. BAKER, L. RICAUD, A. MARTINIÈRE et al., 2005 GAL4-GFP enhancer trap lines for genetic manipulation of lateral root development in Arabidopsis thaliana. J. Exp. Bot. 56: 2433-2442.
34. LI, J., A. M. BRUNNER, R. MEILAN and S. H. STRAUSS, 2008 Matrix attachment region elements have small and variable effects on transgene expression and stability in field-grown Populus. Plant Biotechnol. J. 6: 887-896.
35. LIU, P., N. KOIZUKA, T. M. HOMRICHHAUSEN, J. R. HEWITT, R. C. MARTIN et al., 2005 Large-scale screening of Arabidopsis enhancer-trap lines for seed germination-associated genes. Plant J. 41: 936-944.
36. LUO, Z., and Z. CHEN, 2007 Improperly terminated, unpolyadenylated mRNA of sense transgenes is targeted by RDR6-mediated RNA silencing in Arabidopsis. Plant Cell 19: 943-958.
37. MARKSTEIN, M., C. PITSOULI, C. VILLALTA, S. E. CELNIKER and N. PERRIMON, 2008 Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes. Nat. Genet. 40: 476-483.
38. MATZKE, A. J. M., and M. A. MATZKE, 1998 Position effects and epigenetic silencing of plant transgenes. Curr. Opin. Plant Biol. 1: 142-148.
39. MLYNAROVA, L., A. HRICOVA, A. LOONEN and J. P. NAP, 2003 The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing. Plant Cell 15: 2203-2217.
40. MRAVEC, J., P. SKUPA, A. BAILLY, K. HOYEROVA, P. KRECEK et al., 2009 Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter. Nature 459: 1136-1140.
41. NAGAYA, S., K. YOSHIDA, K. KATO and A.A. SHINMYO, 2001 An insulator element from the sea urchin Hemicentrotus pulcherrimus suppresses variation in transgene expression in cultured tobacco cells. Mol. Genet. Genomic 265: 405-413.
42. NAGAYA, S., K. KATO, Y. NINOMIYA, R. HORIE, M. SEKINE et al., 2005 Expression of randomly integrated single complete copy transgenes does not vary in Arabidopsis thaliana. Plant Cell Physiol. 46: 438-444. (Pubitemid 40507524)
43. NAMCIU, S. J., K. B. BLOCHLINGER and R. E. K. FOURNIER, 1998 Human matrix attachment regions insulate transgene expression from chromosomal position effects in Drosophila melanogaster. Mol. Cell. Biol. 18: 2382-2391.
44. PAPONOV, I. A., W. D. TEALE, M. TREBAR, I. BLILOU and K. PALME, 2005 The PIN auxin efflux facilitators: evolutionary and functional perspectives. Trends Plant Sci. 10: 170-177.
45. PEACH, C., and J. VELTEN, 1991 Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters. Plant Mol. Biol. 17: 49-60.
46. RAAB, J. R., and R. T. KAMAKAKA, 2010 Insulators and promoters: closer than we think. Nat. Rev. Genet. 11: 439-446.
47. ROSIN, F. M., N. WATANABE, J. L. CACAS, N. KATO, J. M. ARROYO et al., 2008 Genome-wide transposon tagging reveals location-dependent effects on transcription and chromatin organization in Arabidopsis. Plant J. 55: 514-525.
48. SARKAR, A., A. ATAPATTU, E. J. BELIKOFF, J. C. HEINRICH, X. LI et al., 2006 Insulated piggybac vectors for insect transgenesis. BMC Biotechnol. 6: 27.
49. SCHUBERT, D., B. LECHTENBERG, A. FORSBACH, M. GILS, S. BAHADUR et al., 2004 Silencing in Arabidopsis T-DNA transformants: the predominant role of a gene-specific RNA sensing mechanism versus position effects. Plant Cell 16: 2561-2572.
50. SGOUROU, A., S. ROUTLEDGE, D. SPATHAS, A. ATHANASSIADOU AND M. N. ANTONIOU, 2009 Physiological levels of HBB transgene expression from S/MAR element-based replicating episomal vectors. J. Biotechnol. 143: 85-94.
51. SILICHEVA, M., A. GOLOVNIN, E. POMERANTSEVA, A. PARSHIKOV, P. GEORGIEV et al., 2010 Drosophila mini-white model system: new insights into positive position effects and the role of transcriptional terminators and gypsy insulator in transgene shielding. Nucleic Acids Res. 38: 39-47.
52. SINGH, J., M. FREELING and D. LISCH, 2008 A position effect on the heritability of epigenetic silencing. PloS Genet. 4: e1000216.
53. SPIKER, S., and W. F. THOMPSON, 1996 Nuclear matrix attachment regions and transgene expression in plants. Plant Physiol. 110: 15-21.
54. STIEF, A., D. M. WINTER, W. H. STRATIING and A. E. SIPPEL, 1989 A nuclear DNA attachment element mediates elevated and position-independent gene activity. Nature 341: 343-345.
55. WANG, X., L. LI, S. DING, X. HUANG, J. ZHANG et al., 2009 Chicken HS4 insulator significantly improves baculovirus-mediated foreign gene expression in insect cells by modifying the structure of neighbouring chromatin in virus minichromosome. J. Biotechnol. 142: 193-199.