Cardiac glycosides ouabain and digoxin interfere with the regulation of glutamate transporter GLAST in astrocytes cultured from neonatal rat brain

Neurochemical Research

Volumn 35, Issue 12, 2010, Pages 2062-2069

  Nguyen, Khoa T D ^a   Buljan, Vlado ^a   Else, Paul L ^b   Pow, David V ^c   Balcar, Vladimir J ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

^b UNIVERSITY OF WOLLONGONG   (Australia)

^c UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Cultured astrocytes; D aspartate; Digoxin; Glutamate transporter GLAST; Na+ K+ ATPase; Ouabain; Rottlerin

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); DIGOXIN; EXCITATORY AMINO ACID TRANSPORTER; GLUTAMIC ACID; OUABAIN; ROTTLERIN;

AMINO ACID TRANSPORT; ANIMAL CELL; ARTICLE; ASTROCYTE; CELL FREE SYSTEM; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; NERVE CELL CULTURE; NEUROTOXICITY; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RAT;

ANIMALS; ANIMALS, NEWBORN; ASTROCYTES; BRAIN; DIGOXIN; EXCITATORY AMINO ACID TRANSPORTER 1; IMMUNOHISTOCHEMISTRY; OUABAIN; RATS; RATS, SPRAGUE-DAWLEY; SODIUM-POTASSIUM-EXCHANGING ATPASE;

RATTUS; STROPHANTHUS GRATUS;

EID: 78651297072     PISSN: 03643190     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11064-010-0274-4     Document Type: Article

References (35)

1. NC Danbolt 2001 Glutamate uptake Prog Neurobiol 65 1 105 1:CAS:528:DC%2BD3MXjsFWns74%3D 10.1016/S0301-0082(00)00067-8 11369436 (Pubitemid 32479155)
2. +-dependent transport of acidic amino acids I the mammalian central nervous system Biol Phar Bull 25 291 301 1:CAS:528:DC%2BD38XkslWltro%3D 10.1248/bpb.25.291 (Pubitemid 39663278)
3. AL Sheldon MB Robinson 2007 The role of glutamate transporters in neurodegenerative diseases and potential opportunities for intervention Neurochem Internat 51 333 355 1:CAS:528:DC%2BD2sXhtFehsLrL 10.1016/j.neuint. 2007.03.012 (Pubitemid 47503456)
4. PM Beart RD O'Shea 2007 Transporters for l-glutamate: an update on their molecular pharmacology and pathological involvement Brit J Pharmacol 150 5 17 1:CAS:528:DC%2BD2sXis1Kisw%3D%3D 10.1038/sj.bjp.0706949 (Pubitemid 46094664)
5. S Duan CM Anderson BA Stein, et al. 1999 Glutamate induces rapid upregulation of astrocyte glutamate transport and cell-surface expression of GLAST J Neurosci 19 23 10193 10200 1:CAS:528:DyaK1MXnslyrtrk%3D 10575016
6. MB Robinson 2002 Regulated trafficking of neurotransmitter transporters: common notes but different melodies J Neurochem 80 1 11 1:CAS:528: DC%2BD38Xjt1Wqtg%3D%3D 10.1046/j.0022-3042.2001.00698.x 11796738 (Pubitemid 34614572)
7. BTS Susarla RP Seal O Zelenaia, et al. 2004 Differential regulation of GLAST immunoreactivity and activity by protein kinase C: evidence for modification of amino and carboxyl termini J Neurochem 91 1151 1163 1:CAS:528:DC%2BD2cXhtFSitr7P 10.1111/j.1471-4159.2004.02791.x 15569258 (Pubitemid 39587453)
8. BTS Susarla MB Robinson 2003 Rottlerin, an inhibitor of protein kinase C-δ (PKC-δ), inhibits astrocytic glutamate transport activity and reduces GLAST immunoreactivity by a mechanism that appears to be PKC-δ-independent J Neurochem 86 635 645 1:CAS:528:DC%2BD3sXlvF2nur4%3D 10.1046/j.1471-4159.2003.01886.x 12859677 (Pubitemid 36897444)
9. JW Shin KTD Nguyen DW Pow, et al. 2009 Distribution of glutamate transporter GLAST in membranes of cultured astrocytes in the presence of glutamate transport substrates and ATP Neurochem Res 34 1758 1766 1:CAS:528:DC%2BD1MXlvFShtb0%3D 10.1007/s11064-009-9982-z 19440835
10. +)-dependent ATPase activity in brain tissue and alters d-aspartate dependent redistribution of glutamate transporter GLAST in cultured astrocytes Neurochem Res 34 1767 1774 1:CAS:528:DC%2BD1MXmslyhtLY%3D 10.1007/s11064-009- 9996-6 19495968
11. F Fonnum 1984 Glutamate: A neurotransmitter in mammalian brain J Neurochem 42 1 11 1:CAS:528:DyaL2cXksFektQ%3D%3D 10.1111/j.1471-4159.1984. tb09689.x 6139418
12. MR Bennett VJ Balcar 1999 Forty years of amino acid transmission in brain Neurochem Int 35 269 280 1:CAS:528:DyaK1MXlslCqsbw%3D 10.1016/S0197-0186(99) 00068-6 10482347 (Pubitemid 29341477)
13. JC Watkins DE Jane 2006 The glutamate story Brit J Pharmacol 147 S1 S100 S108 1:CAS:528:DC%2BD28XisVCmtQ%3D%3D 10.1038/sj.bjp.0706444 (Pubitemid 43077267)
14. + pump in l-glutamate clearance by cultured rat cortical astrocytes Biol Pharm Bull 23 1051 1054 1:CAS:528:DC%2BD3cXmsVektrk%3D 10993203
15. +-ATPase alpha-2 subunit and the glutamate transporters GLAST and GLT in the rat somatosensory cortex Cereb Cortex 12 515 525 1:STN:280:DC%2BD383hsVagtA%3D%3D 10.1093/cercor/12.5.515 11950769
16. +-ATPase in human fetal astrocytes Neurochem Int 50 916 920 1:CAS:528:DC%2BD2sXmvVKmsL8%3D 10.1016/j.neuint.2006.12.015 17316900 (Pubitemid 46962814)
17. EM Rose JCP Koo JE Antflick SM Ahmed S Angers DR Hampson 2009 Glutamate transporter coupling to Na, K-ATPase J Neurosci 29 8143 8155 1:CAS:528:DC%2BD1MXotFKgtbc%3D 10.1523/JNEUROSCI.1081-09.2009 19553454
18. +-ATPase activity and phenytoin concentrations Arch Neurol 32 549 554 125576
19. +-ATPase and its nitric oxide-mediated regulation in a transgenic mouse model of amyotrophic lateral sclerosis J Neurosci 23 43 51 1:CAS:528:DC%2BD3sXktlajtw%3D%3D 12514200
20. AJ Hansen 1985 Effects of anoxia on ion distribution in the brain Physiol Rev 65 101 141 1:CAS:528:DyaL2MXhtFSksL4%3D 3880896
21. T Wieloch RJ Harris L Symon, et al. 1984 Influence of severe hypoglycemia on brain extracellular calcium and potassium activities, energy and phospholipid metabolism J Neurochem 43 160 168 1:CAS:528:DyaL2cXksFaktb4%3D 10.1111/j.1471-4159.1984.tb06692.x 6726243 (Pubitemid 14083330)
22. RJ Vandenberg 1998 Molecular pharmacology and physiology of glutamate transporters in the central nervous system Clin Exp Pharmacol Physiol 25 393 400 1:CAS:528:DyaK1cXkvFeju7o%3D 10.1111/j.1440-1681.1998.tb02221.x 9673812
23. NJ Maragakis JD Rothstein 2004 Glutamate transporters: animal models to neurologic disease. Neurobiol Disease 15 461 473 1:CAS:528:DC%2BD2cXivVKnu74%3D
24. RD O'Shea 2002 Roles and regulation of glutamate transporters in the central nervous system Clin Exp Pharmacol Physiol 29 1018 1023 10.1046/j.1440-1681.2002.03770.x 12366395 (Pubitemid 35177171)
25. VJ Balcar GAR Johnston 1972 The structural specificity of the high affinity uptake of l-glutamate and l-aspartate by rat brain slices J Neurochem 19 2657 2666 1:CAS:528:DyaE3sXmslKhtw%3D%3D 10.1111/j.1471-4159.1972.tb01325.x 4404455
26. +)-dependent ATPase in brain tissue in vitro Clin Exp Pharmacol Physiol 31 762 769 1:CAS:528:DC%2BD2cXhtVCjt7zP 10.1111/j.1440-1681. 2004.04090.x 15566390 (Pubitemid 39556850)
27. GJ Lees W Leong 1994 Brain lesions induced by nonspecific inhibitors of sodium-potassium ATPase Brain Res 649 225 233 1:CAS:528:DyaK2cXksFSjs74%3D 10.1016/0006-8993(94)91068-5 7953637 (Pubitemid 24197216)
28. GJ Lees W Leong 1995 The sodium-potassium inhibitor ouabain is neurotoxic in the rat substantia nigra and striatum Neurosci Lett 188 113 116 1:CAS:528:DyaK2MXkvVaksrw%3D 10.1016/0304-3940(95)11413-Q 7540738
29. +-ATPase inhibitor J Cereb Blood Flow Metabolism 23 62 74 1:CAS:528:DC%2BD38Xpslakt7g%3D
30. HL Scott DV Pow AE Tanneberg, et al. 2002 Aberrant expression of glutamate transporter EAAT1 in Alzheimer's disease J Neurosci 22 RC206 1 5
31. C Rae ML Lawrance LS Dias, et al. 2000 Strategies for studies of neurotoxic mechanisms involving deficient transport of l-glutamate: antisense knockout in rat brain in vivo and changes in the neurotransmitter metabolism following inhibition of glutamate transport in guinea pig brain slice Brain Res Bull 53 373 381 1:CAS:528:DC%2BD3cXovFGqt7s%3D 10.1016/S0361-9230(00)00372-5 11136992 (Pubitemid 32004650)
32. +-ATPase molecular activity and membrane fatty acid composition in mammalian and avian hearts Physiol Pharmacol Zool 79 522 533 1:CAS:528:DC%2BD28XmtlKjsL8%3D 10.1086/502815 (Pubitemid 43846268)
33. CL Poitry-Yamate L Vutskits T Rauen 2002 Neuronal-induced and glutamate-dependent activation of glial glutamate transporter function J Neurochem 82 987 997 1:CAS:528:DC%2BD38XmsF2hu7g%3D 10.1046/j.1471-4159.2002. 01075.x 12358804
34. +-ATPase activity in astrocytes via activation of a distinct subunit highly sensitive to ouabain J Neurochem 69 2132 2137 1:CAS:528:DyaK2sXmvVKkt7o%3D 10.1046/j.1471-4159.1997.69052132.x 9349559
35. +-dependent binding sites on glutamate transporters in frozen sections of rat brain Life Sci 70 991 1001 1:CAS:528:DC%2BD38XovVWisw%3D%3D 10.1016/S0024-3205(01)01478-3 11860158 (Pubitemid 34085091)