-
5
-
-
72549091286
-
-
M.Y. Lee, M.H. Kim, J. Kim, S.H. Kim, B.T. Kim, I.H. Jeong, S. Chang, S.H. Kim, and S.-Y. Chang Bioorg. Med. Chem. Lett. 20 2010 541
-
(2010)
Bioorg. Med. Chem. Lett.
, vol.20
, pp. 541
-
-
Lee, M.Y.1
Kim, M.H.2
Kim, J.3
Kim, S.H.4
Kim, B.T.5
Jeong, I.H.6
Chang, S.7
Kim, S.H.8
Chang, S.-Y.9
-
8
-
-
33645942102
-
-
E.J. Yoo, I. Bae, S.H. Cho, H. Han, and S. Chang Org. Lett. 8 2006 1347
-
(2006)
Org. Lett.
, vol.8
, pp. 1347
-
-
Yoo, E.J.1
Bae, I.2
Cho, S.H.3
Han, H.4
Chang, S.5
-
9
-
-
33749168701
-
-
S. Chang, M. Lee, D.Y. Jung, E.J. Yoo, S.H. Cho, and S.K. Han J. Am. Chem. Soc. 128 2006 12366
-
(2006)
J. Am. Chem. Soc.
, vol.128
, pp. 12366
-
-
Chang, S.1
Lee, M.2
Jung, D.Y.3
Yoo, E.J.4
Cho, S.H.5
Han, S.K.6
-
10
-
-
34248146410
-
-
E.J. Yoo, M. Ahlquist, S.H. Kim, I. Bae, V.V. Fokin, K.B. Sharpless, and S. Chang Angew. Chem., Int. Ed. 46 2007 1730
-
(2007)
Angew. Chem., Int. Ed.
, vol.46
, pp. 1730
-
-
Yoo, E.J.1
Ahlquist, M.2
Kim, S.H.3
Bae, I.4
Fokin, V.V.5
Sharpless, K.B.6
Chang, S.7
-
14
-
-
48249116347
-
-
E.J. Yoo, M. Ahlquist, I. Bae, V.V. Fokin, K.B. Sharpless, and S. Chang J. Org. Chem. 73 2008 5520
-
(2008)
J. Org. Chem.
, vol.73
, pp. 5520
-
-
Yoo, E.J.1
Ahlquist, M.2
Bae, I.3
Fokin, V.V.4
Sharpless, K.B.5
Chang, S.6
-
15
-
-
78651260523
-
-
note
-
2, 1 mM PMSF, 1% Nonidet P-40 and protease inhibitor cocktail).
-
-
-
-
16
-
-
0141732260
-
-
S.M. Khersonsky, D.-W. Jung, T.-W. Kang, D.P. Walsh, H.-S. Moon, H. Jo, E.M. Jacobson, V. Shetty, T.A. Neubert, and Y.-T. Chang J. Am. Chem. Soc. 125 2003 11804
-
(2003)
J. Am. Chem. Soc.
, vol.125
, pp. 11804
-
-
Khersonsky, S.M.1
Jung, D.-W.2
Kang, T.-W.3
Walsh, D.P.4
Moon, H.-S.5
Jo, H.6
Jacobson, E.M.7
Shetty, V.8
Neubert, T.A.9
Chang, Y.-T.10
-
17
-
-
77954894487
-
-
M.H. Kim, Y.L. Choi, J.-N. Heo, Y.K. Min, and S.H. Kim Bull. Korean Chem. Soc. 31 2010 2047
-
(2010)
Bull. Korean Chem. Soc.
, vol.31
, pp. 2047
-
-
Kim, M.H.1
Choi, Y.L.2
Heo, J.-N.3
Min, Y.K.4
Kim, S.H.5
-
18
-
-
78651228489
-
-
note
-
Protein sample preparation and its incubation with 2b. RAW264.7 cells were homogenized (1:5, w/v) in buffer A under sonication. The homogenate was then centrifuged at 10,000g for 15 min and 0.5 ml of the supernatant diluted with buffer A (7 mg/ml) was stirred with buffer A-saturated 2b at 4 °C for 15 h. After incubation, the resins were precipitated by centrifugation at 10,000g for 1 min and washed three times with 1 ml of buffer A. The washed beads were then resuspended with 20 μl of SDS sample buffer (100 mM Tris-HCl, 2% sodium dodecyl sulfate, 1% 2-mercaptoethanol, 2% glycerol, 0.01% bromophenol blue, pH 7.6), incubated at 25 °C for 10 min, and subjected to SDS-PAGE followed by the gel staining.
-
-
-
-
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-
-
78651231787
-
-
note
-
Western blot analysis. RAW264.7 cell lysate (40 μg) were incubated with non-conjugated Affi-Gel 10 or 2b at 4 °C for 15 h and centrifuged at 10,000g for 1 min. The supernatant (unbound fractions) was denatured with SDS sample buffer. Then, the pellets were washed twice with buffer A, resuspended with 20 μl of SDS sample buffer and incubated at 25 °C for 10 min for getting bound fractions. Denaturation of unbound and bound fractions was done by boiling and then samples were subjected to 10% polyacrylamide gels. Electrophoresis was performed using the Mini Protean 3 Cell (Bio-Rad). The resolved proteins were transferred to PVDF membrane (Millipore). The membranes were incubated in blocking buffer (10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1% Tween 20, 3% nonfat dry milk) and then incubated with diluted primary antibodies (1:1000) for 2 h at room temperature. Antibodies used in this study were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz). Following the primary antibody reactions, the membranes were washed with blocking buffer three times (15 min each) and then probed with diluted secondary antibodies (1:2000) for 1 h. The membranes were washed three times (15 min each) and developed with SuperSignal West Femto Maximum Sensitivity Substrate (Pierce Biotechnology) using the LAS-3000 luminescent image analyzer (Fuji Photo Film Co., Ltd).
-
-
-
-
22
-
-
17144384732
-
-
V. García Palacios, L.J. Robinson, C.W. Borysenko, T. Lehmann, S.E. Kalla, and H.C. Blair J. Biol. Chem. 280 2005 13720
-
(2005)
J. Biol. Chem.
, vol.280
, pp. 13720
-
-
García Palacios, V.1
Robinson, L.J.2
Borysenko, C.W.3
Lehmann, T.4
Kalla, S.E.5
Blair, H.C.6
-
23
-
-
33747592377
-
-
M.G. Sørensen, K. Henriksen, M.H. Dziegiel, L.B. Tankó, and M.A. Karsdal DNA Cell Biol. 25 2006 475
-
(2006)
DNA Cell Biol.
, vol.25
, pp. 475
-
-
Sørensen, M.G.1
Henriksen, K.2
Dziegiel, M.H.3
Tankó, L.B.4
Karsdal, M.A.5
-
25
-
-
0027324435
-
-
J.M. Messier, L.M. Shaw, M. Chafel, P. Matsudaira, and A.M. Mercurio Cell Motil. Cytoskeleton 25 1993 223
-
(1993)
Cell Motil. Cytoskeleton
, vol.25
, pp. 223
-
-
Messier, J.M.1
Shaw, L.M.2
Chafel, M.3
Matsudaira, P.4
Mercurio, A.M.5
-
27
-
-
0030754632
-
-
S.G. Babb, P. Matsudaira, M. Sato, I. Correia, and S.S. Lim Cell Motil. Cytoskeleton 37 1997 308
-
(1997)
Cell Motil. Cytoskeleton
, vol.37
, pp. 308
-
-
Babb, S.G.1
Matsudaira, P.2
Sato, M.3
Correia, I.4
Lim, S.S.5
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