Mext regulates the type III secretion system through mexs and ptrc in pseudomonas aeruginosa

Journal of Bacteriology

Volumn 193, Issue 2, 2011, Pages 399-410

  Jin, Yongxin ^a,b   Yang, Hongjiang ^c   Qiao, Mingqiang ^b   Jin, Shouguang ^a,b  

^a UNIVERSITY OF FLORIDA   (United States)

^b NANKAI UNIVERSITY   (China)

^c TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ELASTASE; GENE PRODUCT; OXIDOREDUCTASE; PYOCYANINE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR MEXEF; TRANSCRIPTION FACTOR MEXT; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CONTROLLED STUDY; GENE DELETION; GENE EXPRESSION; GENE OVEREXPRESSION; GENE REPRESSION; MEXS BACTERIAL GENE; MEXT BACTERIAL GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; PTRC BACTERIAL GENE; REGULATORY MECHANISM; SEQUENCE ANALYSIS; TYPE III SECRETION SYSTEM;

BACTERIAL PROTEINS; DNA, BACTERIAL; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; PANCREATIC ELASTASE; PSEUDOMONAS AERUGINOSA; PYOCYANINE; REPRESSOR PROTEINS; SEQUENCE ANALYSIS, DNA; TRANS-ACTIVATORS; VIRULENCE FACTORS;

PSEUDOMONAS AERUGINOSA;

EID: 78650907947     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.01079-10     Document Type: Article

References (59)

1. Ahn, K. S., U. Ha, J. Jia, D. Wu, and S. Jin. 2004. The truA gene of Pseudomonas aeruginosa is required for the expression of type III secretory genes. Microbiology 150:539-547.
2. Anderson, G. G., T. L. Yahr, R. R. Lovewell, and G. A. O'Toole. 2010. The Pseudomonas aeruginosa magnesium transporter MgtE inhibits transcription of the type III secretion system. Infect. Immun. 78:1239-1249.
3. Blackwood, L. L., R. M. Stone, B. H. Iglewski, and J. E. Pennington. 1983. Evaluation of Pseudomonas aeruginosa exotoxin A and elastase as virulence factors in acute lung infection. Infect. Immun. 39:198-201.
4. Bleves, S., C. Soscia, P. Nogueira-Orlandi, A. Lazdunski, and A. Filloux. 2005. Quorum sensing negatively controls type III secretion regulon expression in Pseudomonas aeruginosa PAO1. J. Bacteriol. 187:3898-3902.
5. Brutinel, E., C. Vakulskas, K. Brady, and T. Yahr. 2008. Characterization of ExsA and of ExsA-dependent promoters required for expression of the Pseudomonas aeruginosa type III secretion system. Mol. Microbiol. 68:657-671.
6. Brutinel, E., and T. Yahr. 2008. Control of gene expression by type III secretory activity. Curr. Opin. Microbiol. 11:128-133.
7. Brutinel, E. D., C. A. Vakulskas, and T. L. Yahr. 2010. ExsD inhibits expression of the Pseudomonas aeruginosa type III secretion system by disrupting ExsA self-association and DNA binding activity. J. Bacteriol. 192:1479-1486.
8. Caldwell, C. C., et al. 2009. Pseudomonas aeruginosa exotoxin pyocyanin causes cystic fibrosis airway pathogenesis. Am. J. Pathol. 175:2473-2488.
9. Crowe, K. E., J. A. Bass, V. M. Young, and D. C. Straus. 1982. Antibody response to Pseudomonas aeruginosa exoproducts in cancer patients. J. Clin. Microbiol. 15:115-122.
10. Dacheux, D., I. Attree, C. Schneider, and B. Toussaint. 1999. Cell death of human polymorphonuclear neutrophils induced by a Pseudomonas aeruginosa cystic fibrosis isolate requires a functional type III secretion system. Infect. Immun. 67:6164-6167.
11. Dacheux, D., et al. 2002. Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon. Infect. Immun. 70:3973-3977.
12. Dasgupta, N., G. Lykken, M. Wolfgang, and T. Yahr. 2004. A novel antianti- activator mechanism regulates expression of the Pseudomonas aeruginosa type III secretion system. Mol. Microbiol. 53:297-308.
13. Dumas, J., C. van Delden, K. Perron, and T. Kohler. 2006. Analysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real-time-PCR. FEMS Microbiol. Lett. 254:217-225.
14. Frank, D. W., and B. H. Iglewski. 1991. Cloning and sequence analysis of a trans-regulatory locus required for exoenzyme S synthesis in Pseudomonas aeruginosa. J. Bacteriol. 173:6460-6468.
15. Frisk, A., et al. 2004. Transcriptome analysis of Pseudomonas aeruginosa after Interaction with human airway epithelial cells. Infect. Immun. 72:5433-5438.
16. Fukuda, H., M. Hosaka, K. Hirai, and S. Iyobe. 1990. New norfloxacin resistance gene in Pseudomonas aeruginosa PAO. Antimicrob. Agents Chemother. 34:1757-1761.
17. Garrity-Ryan, L., et al. 2004. The ADP ribosyltransferase domain of Pseudomonas aeruginosa ExoT contributes to its biological activities. Infect. Immun. 72:546-558.
18. Gilligan, P. H. 1991. Microbiology of airway disease in patients with cystic fibrosis. Clin. Microbiol. Rev. 4:35-51.
19. Goodman, A., et al. 2004. A signaling network reciprocally regulates genes associated with acute infection and chronic persistence in Pseudomonas aeruginosa. Dev. Cell 7:745-754.
20. Ha, U., et al. 2004. An in vivo inducible gene of Pseudomonas aeruginosa encodes an anti-ExsA to suppress the type III secretion system. Mol. Microbiol. 54:307-320.
21. Hancock, R., and D. Speert. 2000. Antibiotic resistance in Pseudomonas aeruginosa: Mechanisms and impact on treatment. Drug Resist. Updat. 3:247-255.
22. Hogardt, M., M. Roeder, A. M. Schreff, L. Eberl, and J. Heesemann. 2004. Expression of Pseudomonas aeruginosa exoS is controlled by quorum sensing and RpoS. Microbiology 150:843-851. (Pubitemid 38519305)
23. Jia, J., et al. 2003. c-Jun NH2-terminal kinase-mediated signaling is essential for Pseudomonas aeruginosa ExoS-induced apoptosis. Infect. Immun. 71: 3361-3370.
24. Jin, Y., et al. 2008. Mutational analysis of genes involved in elastin hydrolysis in Pseudomonas aeruginosa. Wei Sheng Wu Xue Bao 48:1623-1628.
25. Kaufman, M. R., et al. 2000. Pseudomonas aeruginosa mediated apoptosis requires the ADP-ribosylating activity of ExoS. Microbiology 146:2531-2541.
26. Kohler, T., S. F. Epp, L. K. Curty, and J.-C. Pechere. 1999. Characterization of MexT, the regulator of the MexE-MexF-OprN multidrug efflux system of Pseudomonas aeruginosa. J. Bacteriol. 181:6300-6305.
27. Kohler, T., et al. 1997. Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa. Mol. Microbiol. 23:345-354.
28. Linares, J. F., et al. 2005. Overexpression of the multidrug efflux pumps MexCD-OprJ and MexEF-OprN is associated with a reduction of type III secretion in Pseudomonas aeruginosa. J. Bacteriol. 187:1384-1391.
29. Lyczak, J. B., C. L. Cannon, and G. B. Pier. 2002. Lung infections associated with cystic fibrosis. Clin. Microbiol. Rev. 15:194-222.
30. Maseda, H., K. Saito, A. Nakajima, and T. Nakae. 2000. Variation of the mexT gene, a regulator of the MexEF-oprN efflux pump expression in wildtype strains of Pseudomonas aeruginosa. FEMS Microbiol. Lett. 192:107-112.
31. McCaw, M., G. Lykken, P. Singh, and T. Yahr. 2002. ExsD is a negative regulator of the Pseudomonas aeruginosa type III secretion regulon. Mol. Microbiol. 46:1123-1133.
32. Mills, S., C. Lim, and D. Cooksey. 1994. Purification and characterization of CopR, a transcriptional activator protein that binds to a conserved domain (cop box) in copper-inducible promoters of Pseudomonas syringae. Mol. Gen. Genet. 244:341-351.
33. Ohman, D. E., S. J. Cryz, and B. H. Iglewski. 1980. Isolation and characterization of Pseudomonas aeruginosa PAO mutant that produces altered elastase. J. Bacteriol. 142:836-842.
34. Plano, G. V. 2010. Coordinating production and distribution: The Pseudomonas aeruginosa ExsACDE regulatory cascade. J. Bacteriol. 192:1477-1478.
35. Rietsch, A., I. Vallet-Gely, S. L. Dove, and J. J. Mekalanos. 2005. ExsE, a secreted regulator of type III secretion genes in Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. U. S. A. 102:8006-8011.
36. Sambrook, J., E. F. Fritsch, and J. Maniatis. 1989. Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
37. Sato, H., J. B. Feix, C. J. Hillard, and D. W. Frank. 2005. Characterization of phospholipase activity of the Pseudomonas aeruginosa type III cytotoxin, ExoU. J. Bacteriol. 187:1192-1195.
38. Schweizer, H. P. 1991. Escherichia-Pseudomonas shuttle vectors derived from pUC18/19. Gene 97:109-121.
39. Schweizer, H. P. 1992. Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker. Mol. Microbiol. 6:1195-1204.
40. Shen, D.-K., et al. 2008. High-cell-density regulation of the Pseudomonas aeruginosa type III secretion system: Implications for tryptophan catabolites. Microbiology 154:2195-2208.
41. Shen, D. K., D. Filopon, L. Kuhn, B. Polack, and B. Toussaint. 2006. PsrA is a positive transcriptional regulator of the type III secretion system in Pseudomonas aeruginosa. Infect. Immun. 74:1121-1129.
42. Shepp, D., I. Tang, M. Ramundo, and M. Kaplan. 1994. Serious Pseudomonas aeruginosa infection in AIDS. J. Acquir. Immune Defic. Syndr. 7:823-831.
43. Sherertz, R. J., and F. A. Sarubbi. 1983. A three-year study of nosocomial infections associated with Pseudomonas aeruginosa. J. Clin. Microbiol. 18: 160-164.
44. Sobel, M. L., S. Neshat, and K. Poole. 2005. Mutations in PA2491 (mexS) promote MexT-dependent mexEF-oprN expression and multidrug resistance in a clinical strain of Pseudomonas aeruginosa. J. Bacteriol. 187:1246-1253. (Pubitemid 40299449)
45. Sundin, C., B. Hallberg, and A. Forsberg. 2004. ADP-ribosylation by exoenzyme T of Pseudomonas aeruginosa induces an irreversible effect on the host cell cytoskeleton in vivo. FEMS Microbiol. Lett. 234:87-91.
46. Thibault, J., E. Faudry, C. Ebel, I. Attree, and S. Elsen. 2009. Anti-activator ExsD forms a 1:1 complex with ExsA to inhibit transcription of type III secretion operons. J. Biol. Chem. 284:15762-15770.
47. Tian, Z.-X., et al. 2009. Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa. Nucleic Acids Res. 37:7546-7559.
48. Tian, Z., et al. 2009. MexT modulates virulence determinants in Pseudomonas aeruginosa independent of the MexEF-OprN efflux pump. Microb. Pathog. 47:237-241.
49. Totten, P. A., and S. Lory. 1990. Characterization of the type a flagellin gene from Pseudomonas aeruginosa PAK. J. Bacteriol. 172:7188-7199.
50. Urbanowski, M. L., E. D. Brutinel, and T. L. Yahr. 2007. Translocation of ExsE into Chinese hamster ovary cells is required for transcriptional induction of the Pseudomonas aeruginosa type III secretion system. Infect. Immun. 75:4432-4439.
51. Urbanowski, M. L., G. L. Lykken, and T. L. Yahr. 2005. A secreted regulatory protein couples transcription to the secretory activity of the Pseudomonas aeruginosa type III secretion system. Proc. Natl. Acad. Sci. U. S. A. 102:9930-9935.
52. Vakulskas, C. A., K. M. Brady, and T. L. Yahr. 2009. Mechanism of transcriptional activation by Pseudomonas aeruginosa ExsA. J. Bacteriol. 191: 6654-6664.
53. Wolfgang, M., V. Lee, M. Gilmore, and S. Lory. 2003. Coordinate regulation of bacterial virulence genes by a novel adenylate cyclase-dependent signaling pathway. Dev. Cell 4:253-263.
54. Wu, W., H. Badrane, S. Arora, H. V. Baker, and S. Jin. 2004. MucAmediated coordination of type III secretion and alginate synthesis in Pseudomonas aeruginosa. J. Bacteriol. 186:7575-7585. (Pubitemid 39463722)
55. Wu, W., and S. Jin. 2005. PtrB of Pseudomonas aeruginosa suppresses the type III secretion system under the stress of DNA damage. J. Bacteriol. 187:6058-6068.
56. Yahr, T., and M. Wolfgang. 2006. Transcriptional regulation of the Pseudomonas aeruginosa type III secretion system. Mol. Microbiol. 62:631-640.
57. Yahr, T. L., A. J. Vallis, M. K. Hancock, J. T. Barbieri, and D. W. Frank. 1998. ExoY, an adenylate cyclase secreted by the Pseudomonas aeruginosa type III system. Proc. Natl. Acad. Sci. U. S. A. 95:13899-13904.
58. Yang, H., et al. 2007. Regulatory role of PopN and its interacting partners in type III secretion of Pseudomonas aeruginosa. J. Bacteriol. 189:2599-2609.
59. Zulianello, L., et al. 2006. Rhamnolipids are virulence factors that promote early infiltration of primary human airway epithelia by Pseudomonas aeruginosa. Infect. Immun. 74:3134-3147.