Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa

Molecular Microbiology

Volumn 23, Issue 2, 1997, Pages 345-354

  Köhler, Thilo ^a   Michéa Hamzehpour, Mehri ^a   Henze, Uta ^a   Gotoh, Naomasa ^b   Curty, Lasta Kocjancic ^a   Pechère, Jean Claude ^a  

^a UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

^b KYOTO PHARMACEUTICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORAMPHENICOL; CIPROFLOXACIN; OUTER MEMBRANE PROTEIN; PYOCYANINE;

ANTIBIOTIC RESISTANCE; ARTICLE; BIOSYNTHESIS; CONTROLLED STUDY; DNA SEQUENCE; METABOLITE; MULTIDRUG RESISTANCE; NONHUMAN; OPEN READING FRAME; OPERON; PHENOTYPE; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0031016991     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1997.2281594.x     Document Type: Article

References (34)

1. Andersen, J., and Delihas, N. (1990) micF RNA binds to the 5′ end of ompF mRNA and to a protein from Escherichia coli. Biochemistry 29: 9249-9256.
2. Essar, D.W., Eberly, L., and Crawfords, I.P. (1990) Identification and characterization of genes for a second anthranilate synthetase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications. J Bacteriol 172: 884-900.
3. Fellay, R., Frey, J., and Krisch, H. (1987) Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52: 147-154.
4. Fukuda, H., Hosaka, M., Hirai, K., and Iyobe, S. (1990) New norfloxacin resistance gene in Pseudomonas aeruginosa PAO. Antimicrob Agents Chemother 34: 1757-1761.
5. Fukuda, H., Hosaka, M., Iyobe, S., Gotoh, N., Nishino, T., and Hirai, K. (1995) nfxC-type quinolone resistance in a clinical isolate of Pseudomonas aeruginosa. Antimicrob Agents Chemother 39: 790-792.
6. Gambino, L., Grachek, S.J., and Miller, P.F. (1993) Overexpression of the MarA positive regulator Is sufficient to confer antibiotic resistance in Escherichia coli. J Bacteriol 175: 2888-2894.
7. Gotoh, N., Itoh, N., Tsujimoto, H., Yamagishi, J., Oyamada, Y., and Nishino, T. (1994) Isolation of OprM-deficient mutants of Pseudomonas aeruginosa by transposon insertion mutagenesis: evidence in involvement in multiple antibiotic resistance. FEMS Microbiol Lett 122: 267-274.
8. Gotoh, N., Tsujimoto, H., Poole, K., Yamaguchi, J.-I., and Nishino, T. (1995) The outer membrane protein OprM of Pseudomonas aeruginosa is encoded by oprK of the mexA-mexB-oprK multidrug resistance operon. Antimicrob Agents Chemother 39: 2567-2569.
9. Hayashi, S., and Wu, H.C. (1990) Lipoproteins in bacteria. J Bioenerg Biomembr 22: 451-471.
10. Hosaka, M., Gotoh, N., and Nishino, T. (1995) Purification of a 54-kilodalton protein (OprJ) produced in NfxB mutants of Pseudomonas aeruginosa and production of a monoclonal antibody specific to OprJ. Antimicrob Agents Chemother 39: 1731-1735.
11. Jones, J.D.G., and Gutterson, N. (1987) An efficient mobilizable cosmid vector, pRK7813, and its use in a rapid method for marker exchange in Pseudomonas fluorescens strain HV37a. Gene 61: 299-306.
12. Köhler, T., Kok, M., Michéa-Hamzehpour, M., Plésiat, P., Gotoh, N., Nishino, T., Kocjanici Curty, L., and Pechère, J.-C. (1996) Multidrug efflux in intrinsic resistance to trimethoprim and sulfamethoxazole in Pseudomonas aeruginosa. Antimicrob Agents Chemother 40: 2288-2290.
13. Li, X.-Z., Livermore, M.D., and Nikaido, H. (1994) Role of efflux pump(s) in intrinsic resistance of Pseudomonas aeruginosa: resistance to tetracycline, chloramphenicol and norfloxacin. Antimicrob Agents Chemother 38: 1732-1741.
14. Li, X.-Z., Nikaido, H., and Poole, K. (1995) Role of MexA-MexB-OprM in antibiotic efflux in Pseudomonas aeruginosa. Antimicrob Agents Chemother 39: 1948-1953.
15. Ma, D., Cook, D.N., Hearst, J.E., and Nikaido, H. (1994) Efflux pumps and drug resistance in Gram-negative bacteria. Trends Microbiol 2: 489-493.
16. Ma, D., Cook, D.N., Alberti, M., Pon, N.G., Nikaido, H., and Hearst, J.E. (1995) Genes acrA and acrB encode a stress-induced efflux system of Escherichia coli. Mol Microbiol 16: 45-56.
17. Masuda, N., Sakagawa, E., and Ohya, S. (1995) Outer membrane proteins for multiple drug resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother 39: 645-649.
18. Michéa-Hamzehpour, M., Lucain, C., and Pechère, J.-C. (1991) Resistance to pefloxacin in Pseudomonas aeruginosa. Antimicrob Agents Chemother 35: 512-518.
19. Michéa-Hamzehpour, M., Pechère, J.-C., Plésiat, P., and Köhler, T. (1995) OprK and OprM define two genetically distinct multidrug efflux systems in Pseudomonas aeruginosa. Antimicrob Agents Chemother 39: 2392-2396.
20. Nikaido, H. (1989) Outer membrane barrier as a mechanism of antimicrobial resistance. Antimicrob Agents Chemother 33: 1831-1836.
21. Okazaki, T., Iyobe, S., Hashimoto, H., and Hirai, K. (1991) Cloning and characterization of a DNA fragment that complements the nfxB mutation in Pseudomonas aeruginosa PAO. FEMS Microbiol Lett 79: 31-36.
22. Poole, K., Heinrichs, D.E., and Neshat, S. (1993a) Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdine. Mol Microbiol 10: 529-544.
23. Poole, K., Krebes, K., McNally, C., and Neshat, S. (1993b) Multiple antibiotic resistance in Pseudomonas aeruginosa: evidence for involvement of an efflux operon. J Bacteriol 175: 7363-7372.
24. Poole, K., Tetro, K., Zhao, Q., Neshat, S., Heinrichs, D., and Bianco, N. (1996a) Expression of the multidrug resistance operon mexA-mexB-oprM in Pseudomonas aeruginosa: mexR encodes a regulator of operon expression. Antimicrob Agents Chemother 40: 2021-2028.
25. Poole, K., Gotoh, N., Tsujimoto, H., Zaho, Q., Wada, A., Yamasaki, T., Neshat, S., Yamagishi, J., and Nishino, T. (1996b) Overexpression of the mexC-mexD-oprJ operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa. Mol Microbiol 21: 713-724.
26. Quandt, J., and Hynes, M.F. (1993) Versatile suicide vectors which allow direct selection for gene replacement in Gramnegative bacteria. Gene 127: 15-21.
27. Sanger, F., Nicklen, S., and Coulson, A.S. (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467.
28. Satake, S., Yoshihara, E., and Nakae, T. (1990) Diffusion of β-lactam antibiotics through liposome membranes reconstituted from purified porins of the outer membrane of Pseudomonas aeruginosa. Antimicrob Agents Chemother 34: 685-690.
29. Shiba, T., Ishiguro, K., Takemoto, N., Koibuchi, H., and Sugimoto, K. (1995) Purification and characterization of the Pseudomonas aeruginosa NfxB protein, the negative regulator of the nfxB gene. J Bacteriol 177: 5872-5877.
30. Simon, R., Priefer, U., and Pühler, A. (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. BioTechnology 1: 784-790.
31. Thornsberry, C., Anhalt, J., Barry, A.L., Gerlach, E.H., Hossom, J., Jones, R.N., Maetsen, J.M., Moellering, R.C., and Norton, R. (1983) Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically. The National Commitee for Clinical Laboratory Standards (NCCLS) 3: 48-56.
32. Trias, J., and Nikaido, H. (1990) Outer membrane protein D2 catalyses facilitated diffusion of carbapenems and penems through the outer membrane of Pseudomonas aeruginosa. Antimicrob Agents Chemother 34: 52-57.
33. 5-oxygenase in Pseudomonas aeruginosa. J Bacteriol 176: 1128-1140.
34. Zhang, C., and Holloway, B.W. (1992) Physical and genetic mapping of the catA region of Pseudomonas aeruginosa. J Gen Microbiol 138: 1097-1107.