Expression of recombinant human interleukin-8 and its purification using a single buffer system

Journal of Immunological Methods

Volumn 364, Issue 1-2, 2011, Pages 77-82

  Wiese, Dana ^a   Schmitz, Katja ^a  

^a KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

Author keywords

Chemokines; Endotoxin removal; Interleukin 8; Protein expression; Purification

Indexed keywords

BUFFER; ENDOTOXIN; HYBRID PROTEIN; PEPTIDASE; RECOMBINANT INTERLEUKIN 8; SERINE; TRITON X 114;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; CELL LYSATE; ION EXCHANGE CHROMATOGRAPHY; LYSIS; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

BUFFERS; CHROMATOGRAPHY, AFFINITY; COST-BENEFIT ANALYSIS; ENDOTOXINS; HUMANS; INTERLEUKIN-8; POLYETHYLENE GLYCOLS; PROTEIN ENGINEERING; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 78650832412     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2010.11.004     Document Type: Article

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