Production of human interleukin-8 expressed in Escherichia coli: From a laboratory scale for in vitro tests via a technical scale for animal studies to a process scale for a GMP-compatible production

Journal of Biotechnology

Volumn 54, Issue 1, 1997, Pages 29-42

  Koltermann, Andre ^a   Boidol, Werner ^b   Daum, Joachim ^b   Scholz, Peter ^b   Donner, Peter ^b  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b BAYER PHARMA AG   (Germany)

Author keywords

Crossflow diafiltration; Downstream processing; Escherichia coli; Fermentation; Production; Purification; Recombinant human Interleukin 8

Indexed keywords

AFFINITY CHROMATOGRAPHY; BIOASSAY; CELLS; COLIFORM BACTERIA; FERMENTATION; FILTRATION; MOLECULAR WEIGHT; PROCESS CONTROL; PURIFICATION; SOLUBILITY;

CROSSFLOW DIAFILTRATION; DOWNSTREAM PROCESSING; ESCHERICHIA COLI; RECOMBINANT HUMAN INTERLEUKIN 8;

GENETIC ENGINEERING;

GUANOSINE PHOSPHATE; INTERLEUKIN 8;

ARTICLE; ESCHERICHIA COLI; FERMENTATION; FILTRATION; NONHUMAN; PRIORITY JOURNAL; PURIFICATION;

BIOREACTORS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; INTERLEUKIN-8; NEUTROPHILS; PLASMIDS; RECOMBINANT PROTEINS; SOLUBILITY; UREA;

ANIMALIA; ESCHERICHIA; ESCHERICHIA COLI; ESCHERICHIA COLI K12;

EID: 0031552378     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(97)01689-1     Document Type: Article

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