Cloning, bacterial expression and biological characterization of recombinant human granulocyte chemotactic protein and differential expression of granulocyte chemotactic protein-2 and epithelial cell-derived neutrophil activating peptide-78 mRNAs

European Journal of Biochemistry

Volumn 243, Issue 3, 1997, Pages 762-769

  Froyen, G ^a   Proost, P ^a   Ronsse, I ^a   Mitera, T ^a   Haelens, A ^a   Wuyts, A ^a   Opdenakker, G ^a   Van Damme, J ^a   Billiau, A ^a  

^a REGA INSTITUTE FOR MEDICAL RESEARCH   (Belgium)

Author keywords

cDNA; Chemokine; Granulocyte chemotactic protein 2; Quantitation; Reverse transcription

Indexed keywords

CHEMOKINE; COMPLEMENTARY DNA; EPITHELIAL DERIVED NEUTROPHIL ACTIVATING FACTOR 78; GAMMA INTERFERON; INTERLEUKIN 1; INTERLEUKIN 8; MESSENGER RNA; RECOMBINANT PROTEIN;

ARTICLE; CALCIUM TRANSPORT; CELL LINE; CHEMOTAXIS; CONTROLLED STUDY; DNA SEQUENCE; DOSE RESPONSE; DRUG COMPARISON; ESCHERICHIA COLI; EXPRESSION VECTOR; FIBROBLAST; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; INFLAMMATION; MOLECULAR CLONING; NEUTROPHIL; OSTEOSARCOMA CELL; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; TUMOR CELL;

BACTERIA (MICROORGANISMS); ENA; ESCHERICHIA COLI;

EID: 0031016517     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00762.x     Document Type: Article

References (34)

1. Ahuja, S. K., Gao, J.‐L. & Murphy, P. M. (1994) Chemokine receptors and molecular mimicry, Immunol. Today 15, 281–287.
2. Baggiolini, M., Dewald, B. & Moser, B. (1994) Interleukin‐8 and related chemotactic cytokines‐CXC aan CC chemokines, Adv. Immunol. 55, 97–79.
3. Ben‐Baruch, A., Michiel, D. F. & Oppenheim, J. J. (1995) Signals and receptors involved in recruitment of inflammatory cells, J. Biol. Chem. 270, 11703–11706.
4. Bleul, C. C., Farzan, M., Choe, H., Parolin, C., Clark‐Lewis, I., Sodroski, J. & Springer, T. A. (1996) The lymphocyte chemoattractant SDF‐1 is a ligand for LESTWfusin and blocks HIV‐1 entry, Nature 382, 829–833.
5. Deuel, T. F., Keim, P. S., Farmer, M., Heinrikson, R. L. (1977) Amino acid sequence of a human platelet factor 4, Proc. Natl Acad. Sci. USA 74, 2256.
6. Doranz, B. J., Rucker, J., Yi, Y., Smyth, R. J., Samson, M., Peiper, S. C., Parmentier, M., Collman, R. G. & Doms, R. W. (1996) A dual‐tropic primary HIV‐1 isolate that uses fusin and the P‐chemokine receptors CKR‐5, CKR‐3, and the CKR‐2b as fusion cofactors, Cell 85, 1149–1158.
7. Grynkiewicz, G., Poenie, M. & Tsien, R. Y. (1985) A new generation of Ca 2+ indicators with greatly improved fluorescence properties, J. Biol. Ckem. 260, 3440–3450.
8. Haskill, S., Peace, A., Morris, J., Sporn, S. A., Anisowicz, A., Lee, S. W., Smith, T., Martin, G., Ralph, P. & Sager, R. (1990) Identification of three related human GRO genes encoding cytokine functions, Proc. Natl Acad. Sci. USA 87, 7732–7736.
9. Hébert, C. A., Luscinskas, F. W., Kiely, J. M., Luis, E. A., Darbonne, W. C., Bennett, G. L., Liu, C. C., Obin, M. S., Gimbrone, M. A. & Baker, J. B. (1990) Endothelial and leukocyte forms of IL‐8. Conversion by thrombin and interaction with neutrophils, J. Immunol. 145, 3033–3040.
10. Hoogenboom, H. R., Griffiths, A. D., Johnson, K. S., Chiswell, D. J., Hudson, P. & Winter, G. (1991) Multi‐subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains, Nucleic Acids Res 19, 4133–4137.
11. Koch, A. E., Kunkel, S. L., Harlow, L. A., Mazarakis, D. D., Haines, G. K., Burdick, M. D., Pope, R. M., Walz, A. & Strieter, R. M. (1994) Epithelial neutrophil activating peptide‐78: A novel chemotactic cytokine for neutrophils in arthritis, J. Clin. Invest. 94, 1012–1018.
12. Luster, A. D. & Ravetch, J. V. (1987) Biochemical characterization of a γ‐interferon inducible cytokine (IP‐10), J. Exp. Med. 166, 1084–1097.
13. Nourshargh, S., Perkins, J. A., Showell, H. J., Matsushima, K., Williams, T. J. & Collins, P. D. (1992) A comparative study of the neutrophil stimulatory activity in vitro and pro‐inflammatory properties in vivo of 72 amino acid and 77 amino acid IL‐8, J. Immunol. 148, 106–111.
14. Oberlin, E., Amara, A., Bachelerie, F., Bessia, C., Virelizier, J.‐L., Arenzana‐Seisdedos, F., Schwartz, O., Heard, J.‐M., Clark‐Lewis, I., Legler, D. F., Loetscher, M., Baggiolini, M. & Moser, B. (1996) The CXC chemokine SDF‐1 is the ligand for LESTWfusin and prevents infection by T‐cell‐line‐adapted HIV‐1, Nature 382, 833–835.
15. Oliveira, I. C., Sciavolino, P. J., Lee, T. H. & Vilcek, J. (1992) Downreg‐ulation of interleukin 8 gene expression in human fibroblasts: unique mechanism of transcriptional inhibition by interferon, Proc. Natl Acad. Sci. USA 89, 9049–9053.
16. Opdenakker, G. & Van Damme, J. (1992) Chemotactic factors, passive invasion and metastasis of cancer cells, Immunol. Today 13, 463–464.
17. Oppenheim, J. J., Zachariae, C. O., Mukaida, N. & Matsushima, K. (1991) Properties of the novel inflammatory supergene “intercrine” cytokine family, Annu. Rev. Immunol. 9, 617–648.
18. Proost, P., De Wolf‐Peeters, C., Conings, R., Opdenakker, G., Billiau, A. & Van Damme, J. (1993a) Identification of a novel granulocyte chemotactic protein (GCP‐2) from human tumor cells; In vitro and in vivo comparison with natural forms of GRO, IP‐10, and IL‐8, J. Immunol. 150, 1000–1010.
19. Proost, P., Wuyts, A. Conings, R., Lenaerts, J.‐P., Billiau, A., Opdenakker, G. & Van Damme, J. (1993b) Human and bovine granulocyte chemotactic protein‐2: Complete amino acid sequence and functional characterization as chemokines, Biochemistry 32, 10170–10177.
20. Proost, P., Wuyts, A. & Van Damme, J. (1996) Human monocyte chemotactic proteins‐2 and ‐3: structural and functional comparison with MCP‐1, J. Leukoc. Biol. 59, 67–74.
21. Rampart, M., Herman, A. G., Grillet, B., Opdenakker, G. & Van Damme, J. (1992) Development and application of a radioimmunoassay for interleukin‐8: Detection of interleukin‐8 in synovial fluids from patients with inflammatory joint disease, Lab. Invest. 66, 512–515.
22. Richmond, A., Balentien, E., Thomas, H. G., Flaggs, G., Barton, D. E., Spiess, J., Bordoni, R., Francke, U. & Derynck, R. (1988) Molecular characterization and chromosomal mapping of melanoma growth stirnulatory activity, a growth factor structurally related to β‐thromboglobulin, EMBO J. 7, 2025–2033.
23. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual, 2nd edn, Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
24. Schägger, H. & von Jagow, G. (1987) Tricine‐sodium dodecyl sulphate‐polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Anal. Biochem. 166, 368–379.
25. Skerra, A. & Plückthun, A. (1988) Assembly of a functional immunoglobulin Fv fragment in Esckerickia coli, Science 240, 1038–1041.
26. Smith, J. B. & Henchman, H. R. (1995) Glucocorticoid‐attenuated response genes encode intercellular mediators, including a new C‐X‐C chemokine, J. Biol. Ckem. 270, 16756–16765.
27. Tekamp‐Olson, P., Gallegos, C., Bauer, D., McClain, J., Sherry, B., Fabre, M., van Deventer, S. & Cerami, A. (1990) Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues, J. Exp. Med. 172, 911–919.
28. Van Damme, J., De Ley, M., Opdenakker, G., Billiau, A., De Somer, P. & Van Beeumen, I. (1985) Homogeneous interferon‐inducing 22 K factor is related to endogenous pyrogen and interleukin‐1, Nature 314, 266–268.
29. Van Damme, J., Van Beeumen, J., Opdenakker, G. & Billiau, A. (1988) A novel, NH 2 ‐terminal sequence‐characterized human monokine pocessing neutrophil chemotactic, skin‐reactive, and granulocytosis‐promoting activity, J. Exp. Med. 167, 1364–1376.
30. Van Damme, J. (1994) Interleukin‐8 and related chemotactic cytokines, in The cytokine handbook ( Thomson, A., ed.) pp. 185–208, Academic Press, London.
31. Walz, A., Peveri, P., Aschauer, H. & Baggiolini, M. (1987) Purification and amino acid sequencing of NAF, a novel neutrophil‐activating factor produced by monocytes, Biochem. Biopkys. Res Commun. 149, 755–761.
32. Walz, A., Burgener, R., Car, B., Baggiolini, M., Kunkel, S. L. & Strieter, R. M. (1991) Structure and neutrophil‐activating properties of a novel inflammatory peptide (ENA‐78) with homology to interleukin 8, J. Exp. Med. 174, 1355–1362.
33. Wuyts, A., Haelens, A., Proost, P., Lenaerts, J.‐P., Conings, R., Opdenakker, G. & Van Damme, J. (1996) Identification of mouse granulocyte chemotactic protein‐2 from fibroblasts and epithelial cells; Functional comparison with natural KC and macrophage inflammatory protein‐2, J. Immunol. 157, 1736–1743.
34. Zhou, N.‐M., Matthys, P., Polacek, C., Fiten, P., Sato, A., Billiau, A. & Froyen, G. (1997) A competitive RT‐PCR method for the quantitative analysis of cytokine mRNAs in mouse tissues, Cytokine, in the press.