High-level expression of extracellular secretion of a β-xylosidase gene from Paecilomyces thermophila in Escherichia coli

Bioresource Technology

Volumn 102, Issue 2, 2011, Pages 1822-1830

  Teng, Chao ^a   Jia, Huiyong ^a   Yan, Qiaojuan ^a   Zhou, Peng ^a   Jiang, Zhengqiang ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Xylosidase; Escherichia coli; Gene cloning; Paecilomyces thermophila; Secretory expression

Indexed keywords

CDNA SEQUENCE; CELL-FREE CULTURE SUPERNATANT; CLONING AND EXPRESSION; CULTURE MEDIUM; E. COLI; EXTRACELLULAR SECRETION; GENE CLONING; GENOMIC DNA; GLYCOSIDE HYDROLASES; HIGH LEVEL EXPRESSION; OPEN READING FRAME; OPTIMAL ACTIVITY; PAECILOMYCES; PROTEIN BANDS; RECOVERY YIELD; SDS-PAGE; SECRETORY EXPRESSION; SHAKE-FLASK CULTURES; SIGNAL PEPTIDE; THERMOPHILIC FUNGUS; XYLOSIDASE;

AMINO ACIDS; CELL CULTURE; CLONING; DNA SEQUENCES; ENZYME ACTIVITY; ESCHERICHIA COLI; FUNGI; GENE ENCODING; SUGARS;

GENE EXPRESSION;

COMPLEMENTARY DNA; GENOMIC DNA; XYLAN 1,4 BETA XYLOSIDASE;

AMINO ACID; COLIFORM BACTERIUM; ENZYME ACTIVITY; FUNGUS; GENE EXPRESSION; GENETIC ANALYSIS; HOMOGENEITY; MOLECULAR ANALYSIS; PEPTIDE; PROTEIN; RECOMBINATION;

ARTICLE; BACTERIUM CULTURE; DNA SEQUENCE; ENZYME RELEASE; ESCHERICHIA COLI; FUNGUS; MOLECULAR WEIGHT; NONHUMAN; OPEN READING FRAME; PAECILOMYCES THERMOPHILA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION;

AMINO ACID SEQUENCE; ASCOMYCOTA; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; EXTRACELLULAR SPACE; GENES, FUNGAL; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; TIME FACTORS; XYLOSIDASES; ZEA MAYS;

ESCHERICHIA COLI; FUNGI;

EID: 78650703050     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2010.09.055     Document Type: Article

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