Purification and Properties of an Extracellular β-Xylosidase from Aspergillus japonicus and Sequence Analysis of the Encoding Gene

Journal of Bioscience and Bioengineering

Volumn 106, Issue 4, 2008, Pages 398-404

  Wakiyama, Motoki ^a   Yoshihara, Koji ^a   Hayashi, Sachio ^a   Ohta, Kazuyoshi ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

xylosidase; Aspergillus japonicus; extracellular enzyme; glycoside hydrolase family 3; xylan

Indexed keywords

AMINATION; AMINES; AMINO ACIDS; CLONING; DIFFUSERS (OPTICAL); ENCODING (SYMBOLS); ENZYMES; GENE ENCODING; NUCLEIC ACIDS; ORGANIC ACIDS; PURIFICATION; SUGARS;

AMINO ACID SEQUENCES; ARABINOFURANOSIDASE; ASPERGILLUS JAPONICUS; ASPERGILLUS NIGER; CDNA ENCODING; CULTURE FILTRATES; ENCODING GENES; EXTRACELLULAR; EXTRACELLULAR ENZYME; EXTRACELLULAR PROTEINS; FILAMENTOUS FUNGUSES; GENE PRODUCTS; GENOMIC DNAS; GLUCOSIDASE; GLYCOSIDE HYDROLASE; GLYCOSIDE HYDROLASE FAMILY 3; MATURE PROTEINS; OPEN READING FRAMES; PICHIA PASTORIS; PRIMARY STRUCTURES; PURIFIED ENZYMES; SEQUENCE ANALYSIS; SIGNAL PEPTIDES; SINGLE COPIES; SOUTHERN BLOT ANALYSIS; XYLAN; XYLOSIDASE;

ENZYME ACTIVITY;

ALPHA ARABINOFURANOSIDASE; XYLAN 1,4 BETA XYLOSIDASE;

ARTICLE; ASPERGILLUS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE; FUNGAL STRAIN; FUNGUS CULTURE; FUNGUS GROWTH; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; SOUTHERN BLOTTING;

ASPERGILLUS; CHROMATOGRAPHY, THIN LAYER; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENOME; GLYCOSIDE HYDROLASES; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; OPEN READING FRAMES; PICHIA; TEMPERATURE; XYLOSIDASES;

ASPERGILLUS JAPONICUS; ASPERGILLUS NIGER; FUNGI; PICHIA PASTORIS; TRITICUM AESTIVUM SUBSP. SPELTA;

EID: 55349106591     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.106.398     Document Type: Article

References (28)

1. Puls J., and Schuseil J. Chemistry of hemicelluloses: relationship between hemicellulose structure and enzymes required for hydrolysis. In: Coughlan M.P., and Hazlewood G.P. (Eds). Hemicellulose and hemicellulase (1993), Portland Press, London 1-27
2. Polizeli M.L.T.M., Rizzatti A.C.S., Monti R., Terenzi H.F., Jorge J.A., and Amorim D.S. Xylanases from fungi: properties and industrial applications. Appl. Microbiol. Biotechnol. 67 (2005) 577-591
3. Rodionova N.A., Tavobilov I.M., and Bezborodov A.M. β-Xylosidase from Aspergillus niger 15: purification and properties. J. Appl. Microbiol. 5 (1983) 300-312
4. Poutanen K., and Puls J. Characteristics of Trichoderma reesei β-xylosidase and its use in the hydrolysis of solubilized xylans. Appl. Microbiol. Biotechnol. 28 (1988) 425-432
5. Rizzatti A.C.S., Jorge J.A., Terenzi H.F., Rechia C.G.V., and Polizeli M.L.T.M. Purification and properties of a thermostable extracellular β-d-xylosidase produced by a thermotolerant Aspergillus phoenicis. J. Ind. Microbiol. Biotechnol. 26 (2001) 156-160
6. van Peij N.N.M.E., Brinkmann J., Vršanská M., Visser J., and de Graaff L.H. β-Xylosidase activity, encoded by xlnD, is essential for complete hydrolysis of xylan by Aspergillus niger but not for induction of the xylanolytic enzyme spectrum. Eur. J. Biochem. 245 (1997) 164-173
7. Kurakake M., Fujii T., Yata M., Okazaki T., and Komaki T. Characteristics of transxylosylation by β-xylosidase from Aspergillus awamori K4. Biochim. Biophys. Acta 1726 (2005) 272-279
8. Kitamoto N., Yoshino S., Ohmiya K., and Tsukagoshi N. Sequence analysis, overexpression, and antisense inhibition of a β-xylosidase gene, xylA, from Aspergillus oryzae KBN616. Appl. Environ. Microbiol. 65 (1999) 20-24
9. Reen F.J., Murray P.G., and Tuohy M.G. Molecular characterisation and expression analysis of the first hemicellulase gene (bxl1) encoding β-xylosidase from the thermophilic fungus Talaromyces emersonii. Biochem. Biophys. Res. Commun. 305 (2003) 579-585
10. Pérez-González J.A., van Peij N.N.M.E., Bezoen A., MacCabe A.P., Ramón D., and de Graaff L.H. Molecular cloning and transcriptional regulation of the Aspergillus nidulans xlnD gene encoding a β-xylosidase. Appl. Environ. Microbiol. 64 (1998) 1412-1419
11. Margolles-Clark E., Tenkanen M., Nakari-Setälä T., and Penttilä M. Cloning of genes encoding α-l-arabinofuranosidase and β-xylosidase from Trichoderma reesei by expression in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 62 (1996) 3840-3846
12. Ito T., Yokoyama E., Sato H., Ujita M., Funaguma T., Furukawa K., and Hara A. Xylosidases associated with the cell surface of Penicillium herquei IFO 4674. J. Biosci. Bioeng. 96 (2003) 354-359
13. Hayashi S., Matsuzaki K., Takasaki Y., Ueno H., and Imada K. Production of β-fructofuranosidase by Aspergillus japonicus. World J. Microbiol. Biotechnol. 8 (1992) 155-159
14. Somogyi M. Notes on sugar determination. J. Biol. Chem. 195 (1952) 19-23
15. Tanaka H., Nakamura T., Hayashi S., and Ohta K. Purification and properties of an extracellular endo-1,4-β-xylanase from Penicillium citrinum and characterization of the encoding gene. J. Biosci. Bioeng. 100 (2005) 623-630
16. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
17. Ohta K., Akimoto H., Matsuda S., Toshimitsu D., and Nakamura T. Molecular cloning and sequence analysis of two endoinulinase genes from Aspergillus niger. Biosci. Biotechnol. Biochem. 62 (1998) 1731-1738
18. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
19. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., and Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28 (1956) 350-356
20. Sambrook J., and Russell D.W. Molecular cloning: a laboratory manual. 3rd ed. (2001), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
21. Chomczynski P., and Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162 (1987) 156-159
22. Tanaka H., Okuno T., Moriyama S., Muguruma M., and Ohta K. Acidophilic xylanase from Aureobasidium pullulans: efficient expression and secretion in Pichia pastoris and mutational analysis. J. Biosci. Bioeng. 98 (2004) 338-343
23. Kozak M. Structural features in eukaryotic mRNAs that modulate the initiation of translation. J. Biol. Chem. 266 (1991) 19867-19870
24. Cubero B., and Scazzocchio C. Two different, adjacent and divergent zinc finger binding sites are necessary for CREA-mediated carbon catabolite repression in the proline gene cluster of Aspergillus nidulans. EMBO J. 13 (1994) 407-415
25. van Peij N.N.M.E., Visser J., and de Graaff L.H. Isolation and analysis of xlnR, encoding a transcriptional activator co-ordinating xylanolytic expression in Aspergillus niger. Mol. Microbiol. 27 (1998) 131-142
26. Bendtsen J.D., Nielsen H., von Heijne G., and Brunak S. Improved prediction of signal peptides: SignalP 3.0. J. Mol. Biol. 340 (2004) 783-795
27. Minic Z., Rihouey C., Trung Do C., Lerouge P., and Jouanin L. Purification and characterization of enzymes exhibiting β-d-xylosidase activities in stem tissues of Arabidopsis. Plant Physiol. 135 (2004) 867-878
28. Hrmova M., De Gori R., Smith B.J., Fairweather J.K., Driguez H., Varghese J.N., and Fincher G.B. Structural basis for broad substrate specificity in higher plant β-d-glucan glucohydrolases. Plant Cell 14 (2002) 1033-1052