Comparison of cytochrome P450 concentrations and metabolic activities in porcine hepatic microsomes prepared with two different methods

Toxicology in Vitro

Volumn 25, Issue 1, 2011, Pages 343-346

  Rasmussen, Martin Krøyer ^a   Ekstrand, Bo ^a   Zamaratskaia, Galia ^b  

^a AARHUS UNIVERSITY   (Denmark)

^b SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

Calcium aggregation method; Cytochrome P450; Liver; Microsomes; Pig; Stability; Ultracentrifugation

Indexed keywords

4 NITROPHENOL HYDROXYLASE; 7 METHOXYRESORUFIN O DEMETHYLASE; 7 PENTOXYRESORUFIN O DEMETHYLASE; BUFFER; COUMARIN HYDROXYLASE; CYTOCHROME P450 1A2; CYTOCHROME P450 2A; CYTOCHROME P450 2E1; EDETIC ACID; ETHOXYRESORUFIN DEETHYLASE; O DEMETHYLASE; OXYGENASE; SUCROSE; TROMETAMOL; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CALCIUM AGGREGATION METHOD; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STABILITY; FREEZE THAWING; INTERMETHOD COMPARISON; LIVER MICROSOME METABOLISM; METHODOLOGY; NONHUMAN; PROTEIN CONTENT; TISSUE PREPARATION; ULTRACENTRIFUGATION; WESTERN BLOTTING;

ANIMALS; ARYL HYDROCARBON HYDROXYLASES; CELL FRACTIONATION; CROSSES, GENETIC; CYTOCHROME P-450 ENZYME SYSTEM; ENZYME STABILITY; FEMALE; FREEZING; ISOENZYMES; MALE; MICROSOMES, LIVER; REPRODUCIBILITY OF RESULTS; SUS SCROFA; TOXICITY TESTS;

SUIDAE; SUS;

EID: 78650523413     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2010.10.007     Document Type: Article

References (16)

1. Asha S., Vidyavatha M. Role of human liver microsomes in in vitro metabolism of drugs - A review. Appl. Biochem. Biotechnol. 2010, 160:1699-1722.
2. Bertz R.J., Granneman G.R. Use of in vitro and in vivo data to estimate the likelihood of metabolic pharmacokinetic interactions. Clin. Pharmacokinet. 1997, 32:210-258.
3. Diaz G.J., Squires E.J. Metabolism of 3-methylindole by porcine liver microsomes: responsible cytochrome P450 enzymes. Toxicol. Sci. 2000, 55:284-292.
4. Ecobichon D.J. Preparation of guinea pig hepatic microsomes: a comparison of three techniques. Res. Commun. Chem. Pathol. Pharmacol. 1976, 14:515-525.
5. Guarnieri M., Finnegan A., DeMarchena O. A procedure for the bulk preparation of brain microsomes with low speed centrifugation. Prep. Biochem. 1976, 6:489-501.
6. Ilyina A., Hernandez Vazquez M.G., Valdez Lara E.L., Chorne Navia R., Chavez Chavez R., Rodriguez Martinez J. Prostaglandin H synthase activity in the presence of calcium ions and mouse skin in vitro. Moscow Univ. Chem. Bull. 2003, 44:57-61.
7. Nelson A.C., Huang W., Moody D.E. Variables in human liver microsome preparation: impact on the kinetics of l-α-acetylmethadol (LAAM) N-demethylation and dextromethorphan O-demethylation. Drug. Metab. Dispos. 2001, 29:319-325.
8. Nicolau-Solano S.I., McGivan J.D., Whittington F.M., Nieuwhof G.J., Wood J.D., Doran O. Relationship between the expression of hepatic but not testicular 3beta-hydroxysteroid dehydrogenase with androstenone deposition in pig adipose tissue. J. Anim. Sci. 2006, 84:2809-2817.
9. Pearce R.E., McIntyre C.J., Madan A., Sanzgiri U., Draper A.J., Bullock P.L., Cook D.C., Burton L.A., Latham J., Nevins C., Parkinson A. Effects of freezing, thawing, and storing human liver microsomes on cytochrome P450 activity. Arch. Biochem. Biophys. 1996, 331:145-169.
10. Rasmussen, M.K., Zamaratskaia, G., Ekstrand, B., 2010. In vitro cytochrome P450 2E1 and CYP2A activities in the presence of testicular steroids. Reprod. Dom. Anim. doi:10.1111/j.1439-0531.2010.01613.x.
11. Ravindranath V., Anandatheerthavarada H.K. Preparation of brain microsomes with cytochrome P450 activity using calcium aggregation method. Anal. Biochem. 1990, 187:310-313.
12. Szotáková B., Baliharová V., Lamka J., Nozinová E., Wsól V., Velík J., Machala M., Neca J., Soucek P., Susová S., Skálová L. Comparison of in vitro activities of biotransformation enzymes in pig, cattle, goat and sheep. Res. Vet. Sci. 2004, 76:43-51.
13. Walawalkar P.S., Serai P.S., Iyer K.R. Isolation and catalytic competence of different animal liver microsomal fractions prepared by calcium-aggregation method. Indian J. Pharm. Sci. 2006, 68:262-265.
14. Yamazaki H., Inoue K., Turvy C.G., Guengerich F.P., Shimada T. Effects of freezing, thawing, and storage of human liver samples on the microsomal contents and activities of cytochrome P450 enzymes. Drug Metab. Dispos. 1997, 25:168-174.
15. Zamaratskaia G., Zlabek V. EROD and MROD as markers of cytochrome P450 1A activities in hepatic microsomes from entire and castrated male pigs. Sensors 2009, 9:2134-2147.
16. Zamaratskaia G., Zlabek V., Chen G., Madej A. Modulation of porcine cytochrome P450 enzyme activities by surgical castration and immunocastration. Animal 2009, 3:1124-1132.