High-throughput analysis of DNA interstrand crosslinks in human peripheral blood mononuclear cells by automated reverse FADU assay

Toxicology

Volumn 280, Issue 1-2, 2011, Pages 53-60

  Dabiak, Małgorzata ^a   Panas, Alicja ^a,c   Steinritz, Dirk ^b   Kehe, Kai ^b   Bürkle, Alexander ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

^b BUNDESWEHR INSTITUTE OF PHARMACOLOGY AND TOXICOLOGY   (Germany)

^c KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

Author keywords

Comet assay; DNA interstrand crosslinks; FADU assay; Melphalan; Sulphur mustard

Indexed keywords

DOUBLE STRANDED DNA; MELPHALAN; MITOMYCIN C; MUSTARD GAS;

ARTICLE; AUTOMATED REVERSE FADU ASSAY; CELL LYSATE; COMET ASSAY; DNA CROSS LINKING; DNA DAMAGE; DNA DENATURATION; DNA DETERMINATION; DOUBLE STRANDED DNA BREAK; HUMAN; HUMAN CELL; NORMAL HUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SINGLE STRANDED DNA BREAK; VENOUS BLOOD;

CROSS-LINKING REAGENTS; DNA BREAKS, DOUBLE-STRANDED; DNA DAMAGE; DNA REPAIR; FLUOROMETRY; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; LEUKOCYTES, MONONUCLEAR;

EID: 78650522346     PISSN: 0300483X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tox.2010.11.007     Document Type: Article

References (22)

1. Balali-Mood M., Hefazi M. Comparison of early and late toxic effects of sulfur mustard in Iranian veterans. Basic Clin. Pharmacol. Toxicol. 2006, 99:273-282.
2. Baumstark-Khan C., Griesenbach U., Rink H. Comparison of DNA strand break induction in CHO cells measured by alkaline elution and by fluorometric analysis of DNA unwinding (FADU). Free Radic. Res. Commun. 1992, 16:381-389.
3. Baumstark-Khan C., Hentschel U., Nikandrova Y., Krug J., Horneck G. Fluorometric analysis of DNA unwinding (FADU) as a method for detecting repair-induced DNA strand breaks in UV-irradiated mammalian cells. J. Photochem. Photobiol. 2000, 72:477-484.
4. Birnboim H.C., Jevcak J.J. Fluorometric method for rapid detection of DNA strand breaks in human white blood cells produced by low doses of radiation. Cancer Res. 1981, 41:1889-1892.
5. Brendler-Schwaab S., Hartmann A., Pfuhler S., Speit G. The in vivo comet assay: use and status in genotoxicity testing. Mutagenesis 2005, 20:245-254.
6. Colvin M.E., Quong J.N. DNA-alkylating events associated with nitrogen mustard based anticancer drugs and the metabolic byproduct Acrolein. Adv. DNA Seq-Spec. Agents 2002, 4:29-46.
7. Dimopoulos M.A., Souliotis V.L., Anagnostopoulos A., Bamia C., Pouli A., Baltadakis I., Terpos E., Kyrtopoulos S.A., Sfikakis P.P. Melphalan-induced DNA damage in vitro as a predictor for clinical outcome in multiple myeloma. Haematologica 2007, 92:1505-1512.
8. Dronkert M.L., Kanaar R. Repair of DNA interstrand cross-links. Mutat. Res. 2001, 486:217-247.
9. Forchhammer L., Brauner E.V., Folkmann J.K., Danielsen P.H., Nielsen C., Jensen A., Loft S., Friis G., Moller P. Variation in assessment of oxidatively damaged DNA in mononuclear blood cells by the comet assay with visual scoring. Mutagenesis 2008, 23:223-231.
10. Hartley J.M., Spanswick V.J., Gander M., Giacomini G., Whelan J., Souhami R.L., Hartley J.A. Measurement of DNA cross-linking in patients on ifosfamide therapy using the single cell gel electrophoresis (comet) assay. Clin. Cancer Res. 1999, 5:507-512.
11. Kee Y., D'Andrea A.D. Expanded roles of the Fanconi anemia pathway in preserving genomic stability. Genes Dev. 2010, 24:1680-1694.
12. Lawley P.D., Phillips D.H. DNA adducts from chemotherapeutic agents. Mutat. Res. 1996, 355:13-40.
13. Merk O., Speit G. Detection of crosslinks with the comet assay in relationship to genotoxicity and cytotoxicity. Environ. Mol. Mutagen. 1999, 33:167-172.
14. McHugh P.J., Spanswick V.J., Hartley J.A. Repair of DNA interstrand crosslinks: molecular mechanisms and clinical relevance. Lancet Oncol. 2001, 2:483-490.
15. Mol M.A., van der Schans G.P., Lohman P.H. Quantification of sulfur mustard-induced DNA interstrand cross-links and single-strand breaks in cultured human epidermal keratinocytes. Mutat. Res. 1993, 294:235-245.
16. Moreno-Villanueva M., Pfeiffer R., Sindlinger T., Leake A., Muller M., Kirkwood T.B., Burkle A. A modified and automated version of the 'Fluorimetric Detection of Alkaline DNA Unwinding' method to quantify formation and repair of DNA strand breaks. BMC Biotechnol. 2009, 9:39.
17. Noll D.M., Mason T.M., Miller P.S. Formation and repair of interstrand cross-links in DNA. Chem. Rev. 2006, 106:277-301.
18. Pfuhler S., Wolf H.U. Detection of DNA-crosslinking agents with the alkaline comet assay. Environ. Mol. Mutagen. 1996, 27:196-201.
19. Roos W.P., Binder A., Bohm L. Determination of the initial DNA damage and residual DNA damage remaining after 12h of repair in eleven cell lines at low doses of irradiation. Int. J. Radiat. Biol. 2000, 76:1493-1500.
20. Roos W., Baumgartner M., Kaina B. Apoptosis triggered by DNA damage O6-methylguanine in human lymphocytes requires DNA replication and is mediated by p53 and Fas/CD95/Apo-1. Oncogene 2004, 23:359-367.
21. Souliotis V.L., Dimopoulos M.A., Sfikakis P.P. Gene-specific formation and repair of DNA monoadducts and interstrand cross-links after therapeutic exposure to nitrogen mustards. Clin. Cancer Res. 2003, 9:4465-4474.
22. Spanswick V.J., Craddock C., Sekhar M., Mahendra P., Shankaranarayana P., Hughes R.G., Hochhauser D., Hartley J.A. Repair of DNA interstrand crosslinks as a mechanism of clinical resistance to melphalan in multiple myeloma. Blood 2002, 100:224-229.