RNA aptazyme-tethered large gold nanoparticles for on-the-spot sensing of the aptazyme ligand

Bioorganic and Medicinal Chemistry Letters

Volumn 21, Issue 1, 2011, Pages 155-159

  Ogawa, Atsushi ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Aptazyme; Biosensor; Gold nanoparticle; Ribozyme

Indexed keywords

GOLD NANOPARTICLE; RIBOZYME; RNA APTAZYME; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; DNA RNA HYBRIDIZATION; ON THE SPOT SENSING; RNA CLEAVAGE; RNA STABILITY; RNA STRUCTURE; ROOM TEMPERATURE; SENSITIVITY AND SPECIFICITY;

APTAMERS, NUCLEOTIDE; BIOSENSING TECHNIQUES; GOLD; LIGANDS; METAL NANOPARTICLES; RNA, CATALYTIC; STATIC ELECTRICITY; TEMPERATURE;

EID: 78650512365     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2010.11.048     Document Type: Article

References (27)

1. W. Zhao, M.A. Brook, and Y. Li ChemBioChem 9 2008 2363
2. R. Elghanian, J.J. Storhoff, R.C. Mucic, R.L. Letsinger, and C.A. Mirkin Science 277 1997 1078
3. K. Sato, K. Hosokawa, and M. Maeda J. Am. Chem. Soc. 125 2003 8102
4. J. Liu, and Y. Lu Angew. Chem., Int. Ed. 45 2006 90
5. C.-C. Huang, Y.-F. Huang, Z. Cao, W. Tan, and H.-T. Chang Anal. Chem. 77 2005 5735
6. X. Xue, F. Wang, and X. Liu J. Am. Chem. Soc. 130 2008 3244
7. W. Zhao, W. Chiuman, M.A. Brook, and Y. Li ChemBioChem 8 2007 727
8. W. Zhao, W. Chiuman, J.C.F. Lam, S.A. McManus, W. Chen, Y. Cui, R. Pelton, M.A. Brook, and Y. Li J. Am. Chem. Soc. 130 2008 3610
9. J. Liu, and Y. Lu J. Am. Chem. Soc. 125 2003 6642
10. J. Liu, and Y. Lu Anal. Chem. 76 2004 1627
11. W. Zhao, J.C.F. Lam, W. Chiuman, M.A. Brook, and Y. Li Small 6 2008 810
12. For example of RNA-Au, see: D.J. Javier, N. Nitin, M. Levy, A. Ellington, and R. Richards-Kortum Bioconjugate Chem. 19 2008 1309
13. A. Ogawa, and M. Maeda Bioorg. Med. Chem. Lett. 18 2008 6517
14. A. Ogawa, and M. Maeda Chem. Lett. 38 2009 848
15. A. Ogawa, and M. Maeda Chem. Commun. 2009 4666
16. K. Sato, M. Onoguchi, Y. Sato, K. Hosokawa, and M. Maeda Anal. Biochem. 350 2006 162
17. W. Zhao, L. Lin, and I.-M. Hsing Bioconjugate Chem. 20 2009 1218
18. G.A. Soukup, G.A.M. Emilsson, and R.R. Breaker J. Mol. Biol. 298 2000 623
19. A. Roth, and R.R. Breaker Methods Mol. Biol. 252 2004 145
20. A. Ogawa, and M. Maeda Bioorg. Med. Chem. Lett. 17 2007 3156
21. A. Ogawa, and M. Maeda ChemBioChem 9 2008 206
22. A. Ogawa, and M. Maeda ChemBioChem 9 2008 2204
23. A. Ogawa ChemBioChem 10 2009 2465
24. The theophylline-dependent cRaptz was efficiently cleaved with theophylline even in the presence of the DNA23, while it was almost not cleaved in the presence of DNA28 (Supplementary Fig. S1), which hybridizes to the cRaptz to prevent the stem I from hybridizing, as shown in Figure 1B.
25. G.S. Manning Q. Rev. Biophys. 11 1978 179
26. The hybridization efficiency of cRaptz to DNA23 on AuNPs was estimated to be ∼20%, which is smaller than that of the cleaved RNA (∼70%), because cRaptz has larger charges.
27. T. Liu, J. Zhao, D. Zhang, and G. Li Anal. Chem. 82 2010 229