The hepatocyte suspension assay is superior to the cultured hepatocyte assay for determining mechanisms of acetaminophen hepatotoxicity relevant to in vivo toxicity

Chemical Research in Toxicology

Volumn 23, Issue 12, 2010, Pages 1855-1858

  Burke, Angela S ^a   MacMillan Crow, Lee Ann ^b   Hinson, Jack A ^b  

^a WIL RESEARCH LABORATORIES   (United States)

^b UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3 NITROTYROSINE; 7 NITROINDAZOLE; CYCLOSPORIN A; CYTOCHROME P450; CYTOCHROME P450 1A2; CYTOCHROME P450 2E1; CYTOCHROME P450 3A4; GLUTATHIONE; INDUCIBLE NITRIC OXIDE SYNTHASE; LACTATE DEHYDROGENASE; N ACETYL 1,4 BENZOQUINONE IMINE; PARACETAMOL; PEROXYNITRITE; REACTIVE OXYGEN METABOLITE;

ANIMAL CELL CULTURE; BINDING SITE; CELL ADHESION; CELL ASSAY; CELL ISOLATION; CELL STRESS; CELL SUSPENSION; CELL VIABILITY; COVALENT BOND; DRUG METABOLISM; DRUG PROTEIN BINDING; ENZYME ACTIVITY; FLUORESCENCE; HISTOPATHOLOGY; HUMAN; INTERMETHOD COMPARISON; LETTER; LIVER CELL; LIVER NECROSIS; LIVER TOXICITY; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRIAL PERMEABILITY; NITRATION; NONHUMAN; OXIDATIVE STRESS; WILD TYPE;

EID: 78650501935     PISSN: 0893228X     EISSN: 15205010     Source Type: Journal    
DOI: 10.1021/tx1003744     Document Type: Letter

References (36)

1. Burke, A. S., MacMillan-Crow, L. A., and Hinson, J. A. (2010) Reactive nitrogen species in acetaminophen-induced mitochondrial damage and toxicity in mouse hepatocytes Chem. Res. Toxicol. 23, 1286-1292
2. Boobis, A. R., Seddon, C. E., Nasseri-Sina, P., and Davies, D. S. (1990) Evidence for a direct role of intracellular calcium in paracetamol toxicity Biochem. Pharmacol. 39, 1277-1281
3. Boobis, A. R., Tee, L. B., Hampden, C. E., and Davies, D. S. (1986) Freshly isolated hepatocytes as a model for studying the toxicity of paracetamol Food Chem. Toxicol. 24, 731-736
4. Hardwick, S. J., Wilson, J. W., Fawthrop, D. J., Boobis, A. R., and Davies, D. S. (1992) Paracetamol toxicity in hamster isolated hepatocytes: the increase in cytosolic calcium accompanies, rather than precedes, loss of viability Arch. Toxicol. 66, 408-412
5. Tee, L. B., Boobis, A. R., Huggett, A. C., and Davies, D. S. (1986) Reversal of acetaminophen toxicity in isolated hamster hepatocytes by dithiothreitol Toxicol. Appl. Pharmacol. 83, 294-314
6. Grewal, K. K. and Racz, W. J. (1993) Intracellular calcium disruption as a secondary event in acetaminophen-induced hepatotoxicity Can. J. Physiol. Pharmacol. 71, 26-33
7. Rafeiro, E., Barr, S. G., Harrison, J. J., and Racz, W. J. (1994) Effects of N-acetylcysteine and dithiothreitol on glutathione and protein thiol replenishment during acetaminophen-induced toxicity in isolated mouse hepatocytes Toxicology 93, 209-224
8. Burcham, P. C. and Harman, A. W. (1990) Mitochondrial dysfunction in paracetamol hepatotoxicity: in vitro studies in isolated mouse hepatocytes Toxicol. Lett. 50, 37-48
9. Burcham, P. C. and Harman, A. W. (1991) Acetaminophen toxicity results in site-specific mitochondrial damage in isolated mouse hepatocytes J. Biol. Chem. 266, 5049-5054
10. Harman, A. W., Mahar, S. O., Burcham, P. C., and Madsen, B. W. (1992) Level of cytosolic free calcium during acetaminophen toxicity in mouse hepatocytes Mol. Pharmacol. 41, 665-670
11. Guillouzo, A. (1998) Liver cell models in in vitro toxicology Environ. Health Perspect. 106 (Suppl 2) 511-532
12. Reid, A. B., Kurten, R. C., McCullough, S. S., Brock, R. W., and Hinson, J. A. (2005) Mechanisms of acetaminophen-induced hepatotoxicity: role of oxidative stress and mitochondrial permeability transition in freshly isolated mouse hepatocytes J. Pharmacol. Exp. Ther. 312, 509-516
13. Hinson, J. A., Pike, S. L., Pumford, N. R., and Mayeux, P. R. (1998) Nitrotyrosine-protein adducts in hepatic centrilobular areas following toxic doses of acetaminophen in mice Chem. Res. Toxicol. 11, 604-607
14. Elfering, S. L., Haynes, V. L., Traaseth, N. J., Ettl, A., and Giulivi, C. (2004) Aspects, mechanism, and biological relevance of mitochondrial protein nitration sustained by mitochondrial nitric oxide synthase Am. J. Physiol. Heart Circ. Physiol. 286, H22-H29
15. Valdez, L. B., Zaobornyj, T., and Boveris, A. (2006) Mitochondrial metabolic states and membrane potential modulate mtNOS activity Biochim. Biophys. Acta 1757, 166-172
16. Wu, D. F., Clejan, L., Potter, B., and Cederbaum, A. I. (1990) Rapid decrease of cytochrome P-450IIE1 in primary hepatocyte culture and its maintenance by added 4-methylpyrazole Hepatology 12, 1379-1389
17. Steward, A. R., Dannan, G. A., Guzelian, P. S., and Guengerich, F. P. (1985) Changes in the concentration of seven forms of cytochrome P-450 in primary cultures of adult rat hepatocytes Mol. Pharmacol. 27, 125-132
18. Singh, G. and Veltri, K. L. (1991) A mechanism for the loss of cytochrome P-450 in primary mouse hepatocytes Mol. Cell. Biochem. 108, 151-156
19. Kocarek, T. A., Schuetz, E. G., and Guzelian, P. S. (1993) Expression of multiple forms of cytochrome P450 mRNAs in primary cultures of rat hepatocytes maintained on matrigel Mol. Pharmacol. 43, 328-334
20. Hinson, J. A., Roberts, D. W., and James, L. P. (2010) Mechanisms of acetaminophen-induced liver necrosis Handb. Exp. Pharmacol. 369-405
21. Cohen, S. D., Pumford, N. R., Khairallah, E. A., Boekelheide, K., Pohl, L. R., Amouzadeh, H. R., and Hinson, J. A. (1997) Selective protein covalent binding and target organ toxicity Toxicol. Appl. Pharmacol. 143, 1-12
22. James, L. P., Donahower, B., Burke, A. S., McCullough, S., and Hinson, J. A. (2006) Induction of the nuclear factor HIF-1alpha in acetaminophen toxicity: evidence for oxidative stress Biochem. Biophys. Res. Commun. 343, 171-176
23. Yan, H. M., Ramachandran, A., Bajt, M. L., Lemasters, J. J., and Jaeschke, H. (2010) The oxygen tension modulates acetaminophen-induced mitochondrial oxidant stress and cell injury in cultured hepatocytes Toxicol. Sci. 117, 515-523
24. Tonge, R. P., Kelly, E. J., Bruschi, S. A., Kalhorn, T., Eaton, D. L., Nebert, D. W., and Nelson, S. D. (1998) Role of CYP1A2 in the hepatotoxicity of acetaminophen: investigations using Cyp1a2 null mice Toxicol. Appl. Pharmacol. 153, 102-108
25. Raucy, J. L., Lasker, J. M., Lieber, C. S., and Black, M. (1989) Acetaminophen activation by human liver cytochromes P450IIE1 and P450IA2 Arch. Biochem. Biophys. 271, 270-283
26. Snawder, J. E., Roe, A. L., Benson, R. W., and Roberts, D. W. (1994) Loss of CYP2E1 and CYP1A2 activity as a function of acetaminophen dose: relation to toxicity Biochem. Biophys. Res. Commun. 203, 532-539
27. Hart, S. G., Cartun, R. W., Wyand, D. S., Khairallah, E. A., and Cohen, S. D. (1995) Immunohistochemical localization of acetaminophen in target tissues of the CD-1 mouse: correspondence of covalent binding with toxicity Fundam. Appl. Toxicol. 24, 260-274
28. Gonzalez, F. J. (2007) The 2006 Bernard B. Brodie Award Lecture. Cyp2e1 Drug Metab. Dispos. 35, 1-8
29. Roberts, D. W., Bucci, T. J., Benson, R. W., Warbritton, A. R., McRae, T. A., Pumford, N. R., and Hinson, J. A. (1991) Immunohistochemical localization and quantification of the 3-(cystein-S-yl)-acetaminophen protein adduct in acetaminophen hepatotoxicity Am. J. Pathol. 138, 359-371
30. Bajt, M. L., Knight, T. R., Lemasters, J. J., and Jaeschke, H. (2004) Acetaminophen-induced oxidant stress and cell injury in cultured mouse hepatocytes: protection by N-acetyl cysteine Toxicol. Sci. 80, 343-349
31. Mitchell, J. R., Jollow, D. J., Potter, W. Z., Gillette, J. R., and Brodie, B. B. (1973) Acetaminophen-induced hepatic necrosis. IV. Protective role of glutathione J. Pharmacol. Exp. Ther. 187, 211-217
32. Jollow, D. J., Mitchell, J. R., Potter, W. Z., Davis, D. C., Gillette, J. R., and Brodie, B. B. (1973) Acetaminophen-induced hepatic necrosis. II. Role of covalent binding in vivo J. Pharmacol. Exp.Ther. 187, 195-202
33. Kidambi, S., Yarmush, R. S., Novik, E., Chao, P., Yarmush, M. L., and Nahmias, Y. (2009) Oxygen-mediated enhancement of primary hepatocyte metabolism, functional polarization, gene expression, and drug clearance Proc. Natl. Acad. Sci. U.S.A. 106, 15714-15719
34. Michael, S. L., Mayeux, P. R., Bucci, T. J., Warbritton, A. R., Irwin, L. K., Pumford, N. R., and Hinson, J. A. (2001) Acetaminophen-induced hepatotoxicity in mice lacking inducible nitric oxide synthase activity Nitric Oxide 5, 432-441
35. Bourdi, M., Masubuchi, Y., Reilly, T. P., Amouzadeh, H. R., Martin, J. L., George, J. W., Shah, A. G., and Pohl, L. R. (2002) Protection against acetaminophen-induced liver injury and lethality by interleukin 10: role of inducible nitric oxide synthase Hepatology 35, 289-298
36. Hinson, J. A., Bucci, T. J., Irwin, L. K., Michael, S. L., and Mayeux, P. R. (2002) Effect of inhibitors of nitric oxide synthase on acetaminophen- induced hepatotoxicity in mice Nitric Oxide 6, 160-167