A Transcriptional Activator Is Part of an SCF Ubiquitin Ligase to Control Degradation of Its Cofactors

Molecular Cell

Volumn 40, Issue 6, 2010, Pages 954-964

  Ouni, Ikram ^a   Flick, Karin ^a   Kaiser, Peter ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN; PROTEIN CBF1; PROTEIN MET31; PROTEIN MET32; PROTEIN MET4; SCF UBIQUITIN LIGASE; TRANSCRIPTION FACTOR; UBIQUITIN PROTEIN LIGASE; UNCLASSIFIED DRUG;

ARTICLE; CELL PROLIFERATION; NONHUMAN; PROTEIN ASSEMBLY; PROTEIN DEGRADATION; PROTEIN STABILITY; UBIQUITINATION; YEAST;

BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTORS; DNA-BINDING PROTEINS; F-BOX PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; UBIQUITIN-PROTEIN LIGASE COMPLEXES;

EID: 78650246421     PISSN: 10972765     EISSN: 10974164     Source Type: Journal    
DOI: 10.1016/j.molcel.2010.11.018     Document Type: Article

References (44)

1. Aghajan M., Jonai N., Flick K., Fu F., Luo M., Cai X., Ouni I., Pierce N., Tang X., Lomenick B., et al. Chemical genetics screen for enhancers of rapamycin identifies a specific inhibitor of an SCF family E3 ubiquitin ligase. Nat. Biotechnol. 2010, 28:738-742.
2. Alberts B. The cell as a collection of protein machines: preparing the next generation of molecular biologists. Cell 1998, 92:291-294.
3. Bai C., Sen P., Hofmann K., Ma L., Goebl M., Harper J.W., Elledge S.J. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 1996, 86:263-274.
4. Barbey R., Baudouin-Cornu P., Lee T.A., Rouillon A., Zarzov P., Tyers M., Thomas D. Inducible dissociation of SCF(Met30) ubiquitin ligase mediates a rapid transcriptional response to cadmium. EMBO J. 2005, 24:521-532.
5. Blaiseau P.L., Thomas D. Multiple transcriptional activation complexes tether the yeast activator Met4 to DNA. EMBO J. 1998, 17:6327-6336.
6. Cardozo T., Pagano M. The SCF ubiquitin ligase: insights into a molecular machine. Nat. Rev. Mol. Cell Biol. 2004, 5:739-751.
7. Chandrasekaran S., Deffenbaugh A.E., Ford D.A., Bailly E., Mathias N., Skowyra D. Destabilization of binding to cofactors and SCFMet30 is the rate-limiting regulatory step in degradation of polyubiquitinated Met4. Mol. Cell 2006, 24:689-699.
8. Fleming J.A., Lightcap E.S., Sadis S., Thoroddsen V., Bulawa C.E., Blackman R.K. Complementary whole-genome technologies reveal the cellular response to proteasome inhibition by PS-341. Proc. Natl. Acad. Sci. USA 2002, 99:1461-1466.
9. Flick K., Ouni I., Wohlschlegel J.A., Capati C., McDonald W.H., Yates J.R., Kaiser P. Proteolysis-independent regulation of the transcription factor Met4 by a single Lys 48-linked ubiquitin chain. Nat. Cell Biol. 2004, 6:634-641.
10. Flick K., Raasi S., Zhang H., Yen J.L., Kaiser P. A ubiquitin-interacting motif protects polyubiquitinated Met4 from degradation by the 26S proteasome. Nat. Cell Biol. 2006, 8:509-515.
11. Ganoth D., Bornstein G., Ko T.K., Larsen B., Tyers M., Pagano M., Hershko A. The cell-cycle regulatory protein Cks1 is required for SCF(Skp2)-mediated ubiquitinylation of p27. Nat. Cell Biol. 2001, 3:321-324.
12. Gardner R.G., Nelson Z.W., Gottschling D.E. Degradation-mediated protein quality control in the nucleus. Cell 2005, 120:803-815.
13. Garriga J., Bhattacharya S., Calbó J., Marshall R.M., Truongcao M., Haines D.S., Graña X. CDK9 is constitutively expressed throughout the cell cycle, and its steady-state expression is independent of SKP2. Mol. Cell. Biol. 2003, 23:5165-5173.
14. Goldberg A.L. Protein degradation and protection against misfolded or damaged proteins. Nature 2003, 426:895-899.
15. Guthrie C., Fink G.R. Guide to Yeast Genetics and Molecular Biology 1991, Volume 194. Academic Press, Inc., San Diego.
16. Hao B., Zheng N., Schulman B.A., Wu G., Miller J.J., Pagano M., Pavletich N.P. Structural basis of the Cks1-dependent recognition of p27(Kip1) by the SCF(Skp2) ubiquitin ligase. Mol. Cell 2005, 20:9-19.
17. Harper J.W. Protein destruction: adapting roles for Cks proteins. Curr. Biol. 2001, 11:R431-R435.
18. Hershko A., Ciechanover A. The ubiquitin system. Annu. Rev. Biochem. 1998, 67:425-479.
19. Kaiser P., Tagwerker C. Is this protein ubiquitinated?. Methods Enzymol. 2005, 399:243-248.
20. Kaiser P., Flick K., Wittenberg C., Reed S.I. Regulation of transcription by ubiquitination without proteolysis: Cdc34/SCF(Met30)-mediated inactivation of the transcription factor Met4. Cell 2000, 102:303-314.
21. Kaiser P., Su N.Y., Yen J.L., Ouni I., Flick K. The yeast ubiquitin ligase SCFMet30: connecting environmental and intracellular conditions to cell division. Cell Div. 2006, 1:16.
22. Kiernan R.E., Emiliani S., Nakayama K., Castro A., Labbé J.C., Lorca T., Nakayama Ki K., Benkirane M. Interaction between cyclin T1 and SCF(SKP2) targets CDK9 for ubiquitination and degradation by the proteasome. Mol. Cell. Biol. 2001, 21:7956-7970.
23. Kuras L., Cherest H., Surdin-Kerjan Y., Thomas D. A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulfur metabolism. EMBO J. 1996, 15:2519-2529.
24. Kuras L., Barbey R., Thomas D. Assembly of a bZIP-bHLH transcription activation complex: formation of the yeast Cbf1-Met4-Met28 complex is regulated through Met28 stimulation of Cbf1 DNA binding. EMBO J. 1997, 16:2441-2451.
25. Kuras L., Rouillon A., Lee T., Barbey R., Tyers M., Thomas D. Dual regulation of the met4 transcription factor by ubiquitin-dependent degradation and inhibition of promoter recruitment. Mol. Cell 2002, 10:69-80.
26. Lee T.A., Jorgensen P., Bognar A.L., Peyraud C., Thomas D., Tyers M. Dissection of combinatorial control by the Met4 transcriptional complex. Mol. Biol. Cell. 2010, 21:456-469.
27. Maniatis T. A ubiquitin ligase complex essential for the NF-kappaB, Wnt/Wingless, and Hedgehog signaling pathways. Genes Dev. 1999, 13:505-510.
28. Patton E.E., Peyraud C., Rouillon A., Surdin-Kerjan Y., Tyers M., Thomas D. SCF(Met30)-mediated control of the transcriptional activator Met4 is required for the G(1)-S transition. EMBO J. 2000, 19:1613-1624.
29. Petroski M.D., Deshaies R.J. Function and regulation of cullin-RING ubiquitin ligases. Nat. Rev. Mol. Cell Biol. 2005, 6:9-20.
30. Petroski M.D., Deshaies R.J. In vitro reconstitution of SCF substrate ubiquitination with purified proteins. Methods Enzymol. 2005, 398:143-158.
31. Reed S.I., Hadwiger J.A., Lörincz A.T. Protein kinase activity associated with the product of the yeast cell division cycle gene CDC28. Proc. Natl. Acad. Sci. USA 1985, 82:4055-4059.
32. Rouillon A., Barbey R., Patton E.E., Tyers M., Thomas D. Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30)complex. EMBO J. 2000, 19:282-294.
33. Schumacher M.M., Choi J.Y., Voelker D.R. Phosphatidylserine transport to the mitochondria is regulated by ubiquitination. J. Biol. Chem. 2002, 277:51033-51042.
34. Seufert W., Jentsch S. Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins. EMBO J. 1990, 9:543-550.
35. Spruck C., Strohmaier H., Watson M., Smith A.P., Ryan A., Krek T.W., Reed S.I. A CDK-independent function of mammalian Cks1: targeting of SCF(Skp2) to the CDK inhibitor p27Kip1. Mol. Cell 2001, 7:639-650.
36. Su N.Y., Flick K., Kaiser P. The F-box protein Met30 is required for multiple steps in the budding yeast cell cycle. Mol. Cell. Biol. 2005, 25:3875-3885.
37. Su N.Y., Ouni I., Papagiannis C.V., Kaiser P. A dominant suppressor mutation of the met30 cell cycle defect suggests regulation of the Saccharomyces cerevisiae Met4-CBF1 transcription complex by Met32. J. Biol. Chem. 2008, 283:11615-11624.
38. Tian Y., Kolb R., Hong J.H., Carroll J., Li D., You J., Bronson R., Yaffe M.B., Zhou J., Benjamin T. TAZ promotes PC2 degradation through a SCFbeta-Trcp E3 ligase complex. Mol. Cell. Biol. 2007, 27:6383-6395.
39. Verma R., McDonald H., Yates J.R., Deshaies R.J. Selective degradation of ubiquitinated Sic1 by purified 26S proteasome yields active S phase cyclin-Cdk. Mol. Cell 2001, 8:439-448.
40. Wheeler G.L., Trotter E.W., Dawes I.W., Grant C.M. Coupling of the transcriptional regulation of glutathione biosynthesis to the availability of glutathione and methionine via the Met4 and Yap1 transcription factors. J. Biol. Chem. 2003, 278:49920-49928.
41. Willems A.R., Schwab M., Tyers M. A hitchhiker's guide to the cullin ubiquitin ligases: SCF and its kin. Biochim. Biophys. Acta 2004, 1695:133-170.
42. Wu C.Y., Roje S., Sandoval F.J., Bird A.J., Winge D.R., Eide D.J. Repression of sulfate assimilation is an adaptive response of yeast to the oxidative stress of zinc deficiency. J. Biol. Chem. 2009, 284:27544-27556.
43. Yen H.C., Elledge S.J. Identification of SCF ubiquitin ligase substrates by global protein stability profiling. Science 2008, 322:923-929.
44. Yen J.L., Su N.Y., Kaiser P. The yeast ubiquitin ligase SCFMet30 regulates heavy metal response. Mol. Biol. Cell 2005, 16:1872-1882.