High-throughput screen for escherichia coli heat shock protein 70 (Hsp70/DnaK): ATPase assay in low volume by exploiting energy transfer

Journal of Biomolecular Screening

Volumn 15, Issue 10, 2010, Pages 1211-1219

  Miyata, Yoshinari ^a,b   Chang, Lyra ^a,b   Bainor, Anthony ^a,b   McQuade, Thomas J ^b   Walczak, Christopher P ^a,b   Zhang, Yaru ^a,b   Larsen, Martha J ^b   Kirchhoff, Paul ^b   Gestwicki, Jason E ^a,b  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

ATPase; fluorescence assay; malachite green; molecular chaperone; phosphate

Indexed keywords

ADENOSINE TRIPHOSPHATASE; CHAPERONE; HEAT SHOCK PROTEIN 70; PHOSPHATE; PROTEIN DNAJ; PROTEIN DNAK; QUINALDINE; REAGENT;

ARTICLE; CHROMATOPHORE; COLORIMETRY; ENERGY TRANSFER; ENZYME ASSAY; ESCHERICHIA COLI; FLUORESCENCE; HIGH THROUGHPUT SCREENING; NONHUMAN; PERFORMANCE; PRIORITY JOURNAL; PROTEIN DETERMINATION;

ADENOSINE TRIPHOSPHATASES; DRUG DISCOVERY; ESCHERICHIA COLI PROTEINS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH-THROUGHPUT SCREENING ASSAYS; HSP40 HEAT-SHOCK PROTEINS; HSP70 HEAT-SHOCK PROTEINS; MOLECULAR CHAPERONES; SMALL MOLECULE LIBRARIES;

ESCHERICHIA COLI; PROKARYOTA;

EID: 78650143677     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057110380571     Document Type: Article

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