UV measurements in microplates suitable for high-throughput protein determination

Analytical Biochemistry

Volumn 313, Issue 2, 2003, Pages 208-215

  Kreusch, Stefan ^a   Schwedler, Sina ^a   Tautkus, Bärbel ^a   Cumme, Gerhard A ^a   Horn, Anton ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

Author keywords

High throughput; Proteins; Spectrophotometry; UV absorption

Indexed keywords

SOAPS (DETERGENTS); SPECTROPHOTOMETRY;

ABSORBANCE COEFFICIENTS; COEFFICIENT OF VARIATION; HIGH THROUGHPUT; INDIVIDUAL CALIBRATIONS; PROTEIN CONCENTRATIONS; PROTEIN DETERMINATION; UV ABSORPTION; UV SPECTROPHOTOMETRIC;

PROTEINS;

BOVINE IMMUNOGLOBULIN; BOVINE SERUM ALBUMIN; BUFFER; CHYMOTRYPSINOGEN; DETERGENT; HEMOGLOBIN; HUMAN IMMUNOGLOBULIN; HUMAN SERUM ALBUMIN; IMMUNOGLOBULIN G; LYSOZYME; PLASMA PROTEIN; RIBONUCLEASE; TRITON X 100;

ACCURACY; ANALYTIC METHOD; ARTICLE; CALIBRATION; DEVICE; LIGHT ABSORPTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONTENT; PROTEIN DETERMINATION; ULTRAVIOLET SPECTROPHOTOMETRY;

EID: 0037442457     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00460-8     Document Type: Article

References (21)

1. Sapan C.V., Lundblad R.L., Price N.C. Colorimetric protein assay techniques. Biotechnol. Appl. Biochem. 29:1999;99-108.
2. Mach H., Volkin D.B., Burke C.J., Middaugh C.R. Ultraviolet absorption spectroscopy. Methods Mol. Biol. 40:1995;91-114.
3. Peterson G.L. Determination of total protein. Methods Enzymol. 91:1983;95-119.
4. Stoscheck C.M. Quantitation of protein. Methods Enzymol. 182:1990;50-68.
5. Lorenzen A., Kennedy S.W. A fluorescence-based protein assay for use with a microplate reader. Anal. Biochem. 214:1993;346-348.
6. D.G. Hafeman, G.M.K. Humphries, Ultraviolet radiation transparent multiassay plates. United States Patent 5, 487, 872 (1996).
7. G. Knebel, Microplatte mit tranparentem Boden. German Patent DE19712484 (1998).
8. N. Kiyokazu, Automatic analyzing device. Japanese Patent Office 60183560 A (1985).
9. D.G. Hafeman, C.T. Chow, Determination of light absorption pathlength in a vertical-beam photometer. United States Patent 6, 320, 662 (2001).
10. D.G. Hafeman, K.L. Crawford, S.J. Gallagher, Multi-assay plate cover for elimination of meniscus. United States Patent 6, 074, 614 (2000).
11. Geiger G., Bernhagen J., Wagner E., Bisswanger H., Brunner H., Vitzthum F. Standardized measurements and differential spectroscopy in microplates. Anal. Biochem. 296:2001;29-40.
12. Fasman G.D. Fasman G.D. Practical Handbook of Biochemistry and Molecular Biology. 1990;CRC Press, Boca Raton, FL.
13. A. Horn, R. Bublitz, S. Kreusch, S. Schwedler, G. Sammler, Probenplatte zur Verwendung in Dialysesystemen. Application for German patent, German Patent Office, Registriernr.101 60 975.2 (2001).
14. Goldfarb A.R., Saidel L.J., Mosovich E. The ultraviolett absorption spectra of proteins. J. Biol. Chem. 193:1951;397-404.
15. Scopes R.K. Measurement of protein by spectrophotometry at 205. nm Anal. Biochem. 59:1974;277-282.
16. Warburg O., Christian W. Isolierung und Kristallisation des Gärungsferments Enolase. Biochem. Z. 1941;310, 385-423.
17. Waddel W.J., Hill C. A simple ultraviolet spectrophotometric method for the determination of protein. J. Lab. Clin. Med. 48:1956;311-314.
18. Wolf P. A critical reappraisal of Waddell's technique for ultraviolet spectrophotometric protein estimation. Anal. Biochem. 129:1983;145-155.
19. Groves W.E., Davis F.C. Jr., Sells B.H. Spectrophotometric determination of microgram quantities of protein without nucleic acid interference. Anal. Biochem. 22:1968;195-210.
20. Dunn D.A., Feygin I. Challenges and solutions to ultra-high-throughput screening assay miniaturization: submicroliter fluid handling. Drug Discov. Today. 5:2000;84-91.
21. Battersby B.J., Trau M. Novel miniaturized systems in high-throughput screening. Trends Biotech. 20:2002;167-173.