Purification and characterization of an exo-type β-N-acetylglucosaminidase from Pseudomonas fluorescens JK-0412

Journal of Applied Microbiology

Volumn 110, Issue 1, 2011, Pages 277-286

  Park, J K ^a   Kim, W J ^a   Park, Y I ^a  

^a The Catholic University of Korea   (South Korea)

Author keywords

Chitin; N acetylglucosamine (GlcNAc); Pseudomonas fluorescens; N acetylglucosaminidase

Indexed keywords

BACTERIA; CHITIN; COST EFFECTIVENESS; ENZYMES; PURIFICATION;

BACTERIAL STRAINS; CHITINOLYTIC ENZYMES; GRAM-NEGATIVE BACTERIA; MINIMAL MEDIAS; N-ACETYLGLUCOSAMINE; N-ACETYLGLUCOSAMINE (GLCNAC); N-ACETYLGLUCOSAMINIDASE; PSEUDOMONAS FLUORESCENS; SOLE CARBON SOURCE; Β-N-ACETYLGLUCOSAMINIDASE;

OLIGOSACCHARIDES;

METAL ION; N ACETYL BETA GLUCOSAMINIDASE;

BACTERIUM; BIODEGRADATION; CHITIN; ENZYME ACTIVITY; GENETIC ANALYSIS; PH; REACTION KINETICS; SECRETION;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM ISOLATION; CARBON SOURCE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SUBSTRATE; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PSEUDOMONAS FLUORESCENS; SEQUENCE ANALYSIS; THERMOSTABILITY;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 78650108011     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2010.04879.x     Document Type: Article

References (39)

1. Badreddine, I., Lafitte, C., Heux, L., Skandalis, N., Spanou, Z., Martinez, Y., Esquerre-Tugaye, M.T., Bulone, V. et al. (2008) Cell wall chitosaccharides are essential components and exposed patterns of the phytopathogenic oomycete Aphanomyces euteiches. Eukaryot Cell 7, 1980-1993.
2. Benhamou, N., Rey, P., Picard, K. and Tirilly, Y. (1999) Ultrastructural and cytochemical aspects of the interaction between the Mycoparasite Pythium oligandrum and soilborne plant pathogens. Phytopathology 89, 506-517.
3. Boldo, J.T., Junges, A., Do Amaral, K.B., Staats, C.C., Vainstein, M.H. and Schrank, A. (2009) Endochitinase CHI2 of the biocontrol fungus Metarhizium anisopliae affects its virulence toward the cotton stainer bug Dysdercus peruvianus. Curr Genet 55, 551-560.
4. Da Silva, C.A., Chalouni, C., Williams, A., Hartl, D., Lee, C.G. and Elias, J.A. (2009) Chitin is a size-dependent regulator of macrophage TNF and IL-10 production. J Immunol 182, 3573-3582.
5. Dube, R., Rook, G.A., Steele, J., Brealey, R., Dwek, R., Rademacher, T. and Lennard-Jones, J. (1990) Agalactosyl IgG in inflammatory bowel disease: correlation with C-reactive protein. Gut 31, 431-434.
6. Einbu, A., Grasdalen, H. and Varum, K.M. (2007) Kinetics of hydrolysis of chitin/chitosan oligomers in concentrated hydrochloric acid. Carbohydr Res 342, 1055-1062.
7. Flach, J., Pilet, P.E. and Jolles, P. (1993) What's new in chitinase research? Experimentia 48, 701-716.
8. He, H., Chen, X., Sun, C., Zhang, Y. and Gao, P. (2006) Preparation and functional evaluation of oligopeptide-enriched hydrolysate from shrimp (Acetes chinensis) treated with crude protease from Bacillus sp. SM98011. Bioresour Technol 97, 385-390.
9. Ichinohe, T., Nagata, N., Strong, P., Tamura, S., Takahashi, H., Ninomiya, A., Imai, M., Odagiri, T. et al. (2007) Prophylactic effects of chitin microparticles on highly pathogenic H5N1 influenza virus. J Med Virol 79, 811-819.
10. Ikeda, M., Miyauchi, K., Mochizuki, A. and Matsumiya, M. (2009) Purification and characterization of chitinase from the stomach of silver croaker Pennahia argentatus. Protein Expr Purif 65, 214-222.
11. Kang, S.C., Park, S. and Lee, D.G. (1999) Purification and characterization of a novel chitinase from the entomopathogenic fungus, Metarhizium anisopliae. J Invertebr Pathol 73, 276-281.
12. Keyhani, N.O. and Roseman, S. (1996) The chitin catabolic cascade in the marine bacterium Vibrio furnissii. Molecular cloning, isolation, and characterization of a periplasmic beta-N-acetylglucosaminidase. J Biol Chem 271, 33425-33432.
13. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
14. Lan, X., Ozawa, N., Nishiwaki, N., Kodaira, R., Okazaki, M. and Shimosaka, M. (2004) Purification, cloning, and sequence analysis of beta-N-acetylglucosaminidase from the chitinolytic bacterium Aeromonas hydrophila strain SUWA-9. Biosci Biotechnol Biochem 68, 1082-1090.
15. Lee, C.G. (2009) Chitin, chitinases and chitinase-like proteins in allergic inflammation and tissue remodeling. Yonsei Med J 50, 22-30.
16. Li, H., Morimoto, K., Katagiri, N., Kimura, T., Sakka, K., Lun, S. and Ohmiya, K. (2002) A novel β-N-acetylglucosaminidase of Clostridium paraputrificum M-21 with high activity on chitobiose. Appl Microbiol Biotechnol 60, 420-427.
17. Lisboa De Marco, J., Valadares-Inglis, M.C. and Felix, C.R. (2004) Purification and characterization of an N-acetylglucosaminidase produced by a Trichoderma harzianum strain which controls Crinipellis perniciosa. Appl Microbiol Biotechnol 64, 70-75.
18. Matsuo, Y., Kurita, M., Park, J.K., Tanaka, K., Nakagawa, T., Kawamukai, M. and Matsuda, H. (1999) Purification, characterization and gene analysis of N-acetylglucosaminidase from Enterobacter sp. G-1. Biosci Biotechnol Biochem 63, 1261-1268.
19. Molloy, C., Cannon, R.D., Sullivan, P.A. and Shepherd, M.G. (1994) Purification and characterization of two forms of N-acetylglucosaminidase from Candida albicans showing widely different outer chain glycosylation. Microbiology 140, 1543-1553.
20. Muzzarelli, R.A. (1997) Human enzymatic activities related to the therapeutic administration of chitin derivatives. Cell Mol Life Sci 53, 131-140.
21. Na, Y.S., Kim, W.J., Kim, S.M., Park, J.K., Lee, S.M., Kim, S.O., Synytsya, A. and Park, Y.I. (2010) Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens. Int Immunopharmacol 10, 364-370.
22. Pasonen, H.L., Lu, J., Niskanen, A.M., Seppanen, S.K., Rytkonen, A., Raunio, J., Pappinen, A., Kasanen, R. et al. (2009) Effects of sugar beet chitinase IV on root-associated fungal community of transgenic silver birch in a field trial. Planta 230, 973-983.
23. Richter, C.A., Wright-Osment, M.K., Zajicek, J.L., Honeyfield, D.C. and Tillitt, D.E. (2009) Quantitative polymerase chain reaction (PCR) assays for a bacterial thiaminase I gene and the thiaminase-producing bacterium Paenibacillus thiaminolyticus. J Aquat Anim Health 21, 229-238.
24. Rogers, H.J., Taylor, C., Rayter, S. and Ward, J.B. (1984) Purification and properties of autolytic endo-beta-N-acetylglucosaminidase and the N-acetylmuramyl-L-alanine amidase from Bacillus subtilis strain 168. J Gen Microbiol 130, 2395-2402.
25. Sakai, K., Yokota, A., Kurokawa, H., Wakayama, M. and Moriguchi, M. (1998) Purification and characterization of three thermostable endochitinases of a noble Bacillus strain, MH-1, isolated from chitin-containing compost. Appl Environ Microbiol 64, 3397-3402.
26. Salvatore, S., Heuschkel, R., Tomlin, S., Davies, S.E., Edwards, S., Walker-Smith, J.A., French, I. and Murch, S.H. (2000) A pilot study of N-acetyl glucosamine, a nutritional substrate for glycosaminoglycan synthesis, in paediatric chronic inflammatory bowel disease. Aliment Pharmacol Ther 14, 1567-1579.
27. Segel, I.H. (1976) Biochemical Calculations, 2nd edn. pp. 236-264. New York: John Wiley & Sons, Inc..
28. Shibata, Y., Honda, I., Justice, J.P., Van Scott, M.R., Nakamura, R.M. and Myrvik, Q.N. (2001) Th1 adjuvant N-acetyl-D-glucosamine polymer up-regulates Th1 immunity but down-regulates Th2 immunity against a mycobacterial protein (MPB-59) in interleukin-10-knockout and wild-type mice. Infect Immun 69, 6123-6130.
29. Shikhman, A.R., Amiel, D., D'Lima, D., Hwang, S.B., Hu, C., Xu, A., Hashimoto, S., Kobayashi, K. et al. (2005) Chondroprotective activity of N-acetylglucosamine in rabbits with experimental osteoarthritis. Ann Rheum Dis 64, 89-94.
30. Sorokulova, I., Krumnow, A., Globa, L. and Vodyanoy, V. (2009) Efficient decomposition of shrimp shell waste using Bacillus cereus and Exiguobacterium acetylicum. J Ind Microbiol Biotechnol 36, 1123-1126.
31. Suginta, W., Vongsuwan, A., Songsiriritthigul, C., Svasti, J. and Prinz, H. (2005) Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS. FEBS J 272, 3376-3386.
32. Suzuki, K., Mikami, T., Okawa, Y., Tokoro, A., Suzuki, S. and Suzuki, M. (1986) Antitumor effect of hexa-N-acetylchitohexaose and chitohexaose. Carbohydr Res 151, 403-408.
33. Valdivia, A., Perez, Y., Dominguez, A., Caballero, J., Gomez, L., Schacht, E.H. and Villalonga, R. (2005) Improved anti-inflammatory and pharmacokinetic properties for superoxide dismutase by chemical glycosidation with carboxymethylchitin. Macromol Biosci 5, 118-123.
34. Valisena, S., Varaldo, P.E. and Satta, G. (1991) Staphylococcal endo-beta-N-acetylglucosaminidase inhibits response of human lymphocytes to mitogens and interferes with production of antibodies in mice. J Clin Invest 87, 1969-1976.
35. Voss-Foucart, C.J.M.F. (1991) Chitin biomass and production in the marine environment. Biochem Syst Ecol 19, 347-356.
36. Wang, S.L., Lin, T.Y., Yen, Y.H., Liao, H.F. and Chen, Y.J. (2006) Bioconversion of shellfish chitin wastes for the production of Bacillus subtilis W-118 chitinase. Carbohydr Res 341, 2507-2515.
37. Wang, S.L., Lin, H.T., Liang, T.W., Chen, Y.J., Yen, Y.H. and Guo, S.P. (2008) Reclamation of chitinous materials by bromelain for the preparation of antitumor and antifungal materials. Bioresour Technol 99, 4386-4393.
38. Wu, Y., Preston, G. and Bianco, A.E. (2008) Chitinase is stored and secreted from the inner body of microfilariae and has a role in exsheathment in the parasitic nematode Brugia malayi. Mol Biochem Parasitol 161, 55-62.
39. Yem, D.W. and Wu, H.C. (1976) Purification and properties of beta-N-acetylglucosaminidase from Escherichia coli. J Bacteriol 125, 324-331.