Purification and biochemical characterization of recombinant alcohol dehydrogenase from the psychrophilic bacterium Pseudomonas frederiksbergensis

Journal of Polymers and the Environment

Volumn 18, Issue 4, 2010, Pages 617-625

  Abdel Megeed, Ahmed ^a   Aboul Soud, Mourad A M ^a   Mueller, Rudolf ^b   Rudolf, Fredua Agyeman ^b   Al Deyab, Salem S ^a  

^a KING SAUD UNIVERSITY   (Saudi Arabia)

^b HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Alcohol dehydrogenase; Bioremediation; Enzyme purification; Pseudomonasfrederiksbergensis; Psychrophilic bacteria

Indexed keywords

16S RRNA; ALCOHOL DEHYDROGENASE; ALIPHATIC ALCOHOL; AMMONIUM SULPHATE; APPARENT K; BIOCHEMICAL CHARACTERIZATION; BIOTECHNOLOGICAL APPLICATIONS; COMPETENT CELLS; CRUDE EXTRACT; DENMARK; DIRECTED EVOLUTION; E. COLI; ENZYME PURIFICATION; EXPERIMENTAL EVIDENCE; GEL FILTRATION ANALYSIS; GEL-FILTRATION CHROMATOGRAPHY; HALF LIVES; ICE SAMPLES; METAGENOMES; N-ALKANES; NATIVE FORMS; OPTIMUM PH; OPTIMUM TEMPERATURE; OVER-EXPRESSION; PROPAN-1-OL; PSEUDOMONASFREDERIKSBERGENSIS; PSYCHROPHILIC BACTERIA; Q-SEPHAROSE; REACTION CONDITIONS; RECOMBINANT PROTEIN; SDS-PAGE; SECONDARY ALCOHOLS; SEQUENCE ANALYSIS; SPECIFIC ACTIVITY; SUBSTRATE SPECIFICITY; THERMAL STABILITY;

AMMONIUM COMPOUNDS; BACTERIOLOGY; BIODEGRADATION; BIOREMEDIATION; BIOTECHNOLOGY; CATALYSTS; COLUMN CHROMATOGRAPHY; DIFFUSERS (OPTICAL); ENZYMES; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GELS; MOLECULAR MASS; PARAFFINS; POLLUTION; PURIFICATION; RNA;

BACTERIA;

ALCOHOL; BACTERIUM; BIOCHEMISTRY; BIODEGRADATION; BIOREMEDIATION; BIOTRANSFORMATION; ENZYME ACTIVITY; HOMOGENEITY; PH; REACTION KINETICS; REACTION RATE; RECOMBINATION; SUBSTRATE; ULTRAFILTRATION;

DENMARK;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS FREDERIKSBERGENSIS;

EID: 78650075432     PISSN: 15662543     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10924-010-0205-0     Document Type: Article

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