Rapid isolation of high-affinity human antibodies against the tumor vascular marker Endosialin/TEM1, using a paired yeast-display/secretory scFv library platform

Journal of Immunological Methods

Volumn 363, Issue 2, 2011, Pages 221-232

  Zhao, Aizhi ^a   Nunez Cruz, Selene ^a   Li, Chunsheng ^a   Coukos, George ^a   Siegel, Don L ^a   Scholler, Nathalie ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Biobody; Flow cytometry; In vivo targeting; Ovarian cancer; Tumor vascular markers; Yeast display scFv

Indexed keywords

BIOBODY 78; ENDOSIALIN; RECOMBINANT ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIANGIOGENIC ACTIVITY; ANTIBODY AFFINITY; ANTIBODY ISOLATION; ANTIBODY LIBRARY; ANTIBODY PRODUCTION; ANTIBODY SCREENING; ANTIGEN SPECIFICITY; ARTICLE; BACTERIOPHAGE; BIOTINYLATION; CONTROLLED STUDY; DRUG PROTEIN BINDING; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; FLOW CYTOMETRY; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR STABILITY; MOUSE; NONHUMAN; OVARY CANCER; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN EXPRESSION; TARGET CELL; THROMBOCYTOPENIC PURPURA;

ANIMALS; ANTIBODY AFFINITY; ANTIGENS, CD; ANTIGENS, NEOPLASM; CELL LINE, TUMOR; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; FLOW CYTOMETRY; GENETIC VECTORS; HUMANS; MICE; OVARIAN NEOPLASMS; PEPTIDE LIBRARY; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SINGLE-CHAIN ANTIBODIES; TRANSFORMATION, GENETIC;

EID: 78650003611     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2010.09.001     Document Type: Article

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