Yeast DEL assay detects protection against radiation-induced cytotoxicity and genotoxicity: Adaptation of a microtiter plate version

Radiation Research

Volumn 174, Issue 6 A, 2010, Pages 719-726

  Hafer, Kurt ^a   Rivina, Yelena ^a   Schiestl, Robert H ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 (3 AMINOPROPYLAMINO)ETHANETHIOL; ACETYLCYSTEINE; ASCORBIC ACID; BROXURIDINE; DIMETHYL SULFOXIDE; TEMPOL;

ARTICLE; ASSAY; CELL KILLING; CELL PROTECTION; CONTROLLED STUDY; CYTOTOXICITY; DEL ASSAY; DRUG EFFICACY; GENE DELETION; GENOTOXICITY; HIGH THROUGHPUT SCREENING; IONIZING RADIATION; MICROTITER PLATE ASSAY; NONHUMAN; PRIORITY JOURNAL; RADIATION DOSE; RADIATION PROTECTION; RADIOSENSITIZATION; SACCHAROMYCES CEREVISIAE; VALIDITY; YEAST CELL;

CELL SURVIVAL; GENE DELETION; MERCAPTOETHYLAMINES; RADIATION-PROTECTIVE AGENTS; RADIATION-SENSITIZING AGENTS; REACTIVE OXYGEN SPECIES; SACCHAROMYCES CEREVISIAE;

EID: 78649991992     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.1667/RR2059.1     Document Type: Article

References (49)

1. J. P. Pouget and S. J. Mather, General aspects of the cellular response to low- and high-LET radiation. Eur. J. Nucl. Med. 28, 541-561 (2001).
2. D. W. Moeller, Environmental health physics: 50 years of progress. Health Phys. 88, 676-696 (2005).
3. A. Aroua, P. Trueb, J. P. Vader, J. F. Valley and F. R. Verdun, Exposure of the Swiss population by radiodiagnostics: 2003 review. Health Phys. 92, 442-448 (2007).
4. R. Wakeford, Occupational exposure, epidemiology and compensation. Occup. Med. (Lond.) 56, 173-179 (2006).
5. J. T. Bushberg, L. A. Kroger, M. B. Hartman, E. M. Leidholdt, Jr., K. L. Miller, R. Derlet and C. Wraa, Nuclear/radiological terrorism: Emergency department management of radiation casualties. J. Emerg. Med. 32, 71-85 (2007).
6. D. J. Brenner, R. Doll, D. T. Goodhead, E. J. Hall, C. E. Land, J. B. Little, J. H. Lubin, D. L. Preston, R. J. Preston and M. Zaider, Cancer risks attributable to low doses of ionizing radiation: Assessing what we really know. Proc. Natl. Acad. Sci. USA 100, 13761-13766 (2003).
7. J. S. Puskin, What can epidemiology tell us about risks at low doses? Radiat. Res. 169, 122-124 (2008).
8. K. L. Mossman, Economic and policy considerations drive the LNT debate. Radiat. Res. 169, 245 (2008).
9. B. E. Leonard, Common sense about the linear no-threshold controversy-give the general public a break. Radiat. Res. 169, 245-246 (2008).
10. L. E. Feinendegen, H. Paretzke and R. D. Neumann, Two principal considerations are needed after low doses of ionizing radiation. Radiat. Res. 169, 247-248 (2008).
11. M. Tubiana, A. Arengo, D. Averbeck and R. Masse, Low-dose risk assessment. Radiat. Res. 167, 742-744 (2007).
12. G. C. Jagetia, Radioprotective potential of plants and herbs against the effects of ionizing radiation. J. Clin. Biochem. Nutr. 40, 74-81 (2007).
13. J. F. Weiss and M. R. Landauer, Radioprotection by antioxidants. Ann. NY Acad. Sci. 899, 44-60 (2000).
14. C. Borek, Antioxidants and radiation therapy. J. Nutr. 134, 3207S-3209S (2004).
15. A. Ito, H. Nakano, Y. Kusano, R. Hirayama, Y. Furusawa, C. Murayama, T. Mori, Y. Katsumura and K. Shinohara, Contribution of indirect action to radiation-induced mammalian cell inactivation: dependence on photon energy and heavy-ion LET. Radiat. Res. 165, 703-712 (2006).
16. S. J. Hosseinimehr, Trends in the development of radioprotective agents. Drug Discov. Today 12, 794-805 (2007).
17. E. von Hofe, R. Brent and A. R. Kennedy, Inhibition of X-rayinduced exencephaly by protease inhibitors. Radiat. Res. 123, 108-111 (1990).
18. E. J. Ainsworth, From endotoxins to newer immunomodulators: survival-promoting effects of microbial polysaccharide complexes in irradiated animals. Pharmacol. Ther. 39, 223-241 (1988).
19. L. G. Burdelya, V. I. Krivokrysenko, T. C. Tallant, E. Strom, A. S. Gleiberman, D. Gupta, O. V. Kurnasov, F. L. Fort, A. L. Osterman and A. V. Gudkov, An agonist of toll-like receptor 5 has radioprotective activity in mouse and primate models. Science 320, 226-230 (2008).
20. W. M. Bonner, Low-dose radiation: thresholds, bystander effects, and adaptive responses. Proc. Natl. Acad. Sci. USA 100, 4973-4975 (2003).
21. J. Carmichael, W. G. DeGraff, A. F. Gazdar, J. D. Minna and J. B. Mitchell, Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of radiosensitivity. Cancer Res. 47, 943-946 (1987). (Pubitemid 17027906)
22. Y. Kataoka, J. S. Murley, K. L. Baker and D. J. Grdina, Relationship between phosphorylated histone H2AX formation and cell survival in human microvascular endothelial cells (HMEC) as a function of ionizing radiation exposure in the presence or absence of thiol-containing drugs. Radiat. Res. 168, 106-114 (2007).
23. S. Meunier, M. Desage-El Murr, S. Nowaczyk, T. Le Gall, S. Pin, J. P. Renault, D. Boquet, C. Creminon, E. Saint-Aman and C. Mioskowski, A powerful antiradiation compound revealed by a new high-throughput screening method. Chembiochem 5, 832-840 (2004).
24. R. H. Schiestl, Nonmutagenic carcinogens induce intrachromosomal recombination in yeast. Nature 337, 285-288 (1989).
25. R. J. Brennan and R. H. Schiestl, Persistent genomic instability in the yeast Saccharomyces cerevisiae induced by ionizing radiation and DNA-damaging agents. Radiat. Res. 155, 768-777 (2001).
26. A. J. Bishop and R. H. Schiestl, Homologous recombination and its role in carcinogenesis. J. Biomed. Biotechnol. 2, 75-85 (2002).
27. N. Hontzeas, K. Hafer and R. H. Schiestl, Development of a microtiter plate version of the yeast DEL assay amenable to highthroughput toxicity screening of chemical libraries. Mutat. Res. 634, 228-234 (2007).
28. W. W. Ku, J. Aubrecht, R. J. Mauthe, R. H. Schiestl and A. J. Fornace, Jr., Genetic toxicity assessment: Employing the best science for human safety evaluation Part VII: Why not start with a single test: A transformational alternative to genotoxicity hazard and risk assessment. Toxicol. Sci. 99, 20-25 (2007).
29. R. H. Schiestl, R. D. Gietz, R. D. Mehta and P. J. Hastings, Carcinogens induce intrachromosomal recombination in yeast. Carcinogenesis 10, 1445-1455 (1989).
30. A. Galli and R. H. Schiestl, On the mechanism of UV and gamma-ray-induced intrachromosomal recombination in yeast cells synchronized in different stages of the cell cycle. Mol. Gen. Genet. 248, 301-310 (1995).
31. A. Slavotinek, T. J. McMillan and C. M. Steel, Measurement of radiation survival using the MTT assay. Eur. J. Cancer 30A, 1376-1382 (1994).
32. D. Banasiak, A. R. Barnetson, R. A. Odell, H. Mameghan and P. J. Russell, Comparison between the clonogenic, MTT, and SRB assays for determining radiosensitivity in a panel of human bladder cancer cell lines and a ureteral cell line. Radiat. Oncol. Investig. 7, 77-85 (1999).
33. C. E. Hellweg, A. Arenz and C. Baumstark-Khan, Assessment of space environmental factors by cytotoxicity bioassays. Acta Astronaut. 60, 525-533 (2007).
34. S. Koyama, S. Kodama, K. Suzuki, T. Matsumoto, T. Miyazaki and M. Watanabe, Radiation-induced long-lived radicals which cause mutation and transformation. Mutat. Res. 421, 45-54 (1998).
35. A. Ueno, D. Vannais, M. Lenarczyk and C. A. Waldren, Ascorbate, added after irradiation, reduces the mutant yield and alters the spectrum of CD592 mutations in A(L) cells irradiated with high LET carbon ions. J. Radiat. Res. (Tokyo) 43 (Suppl.), S245-249 (2002).
36. C. A. Waldren, D. B. Vannais and A. M. Ueno, A role for longlived radicals (LLR) in radiation-induced mutation and persistent chromosomal instability: counteraction by ascorbate and RibCys but not DMSO. Mutat. Res. 551, 255-265 (2004).
37. H. Zhou, G. Randers-Pehrson, C. R. Geard, D. J. Brenner, E. J. Hall and T. K. Hei, Interaction between radiation-induced adaptive response and bystander mutagenesis in mammalian cells. Radiat. Res. 160, 512-516 (2003).
38. S. M. Hahn, C. M. Krishna, A. Samuni, W. DeGraff, D. O. Cuscela, P. Johnstone and J. B. Mitchell, Potential use of nitroxides in radiation oncology. Cancer Res. 54, 2006s-2010s (1994).
39. W. G. DeGraff, M. C. Krishna, D. Kaufman and J. B. Mitchell, Nitroxide-mediated protection againstX-ray- and neocarzinostatininduced DNA damage. Free Radic. Biol. Med. 13, 479-487 (1992).
40. D. J. Grdina, N. Shigematsu, P. Dale, G. L. Newton, J. A. Aguilera and R. C. Fahey, Thiol and disulfide metabolites of the radiation protector and potential chemopreventive agent WR- 2721 are linked to both its anti-cytotoxic and anti-mutagenic mechanisms of action. Carcinogenesis 16, 767-774 (1995).
41. D. J. Grdina, B. Nagy, C. K. Hill, R. L. Wells and C. Peraino, The radioprotector WR1065 reduces radiation-induced mutations at the hypoxanthine-guanine phosphoribosyl transferase locus in V79 cells. Carcinogenesis 6, 929-931 (1985). (Pubitemid 15064158)
42. J. S. Murley and D. J. Grdina, The effects of cycloheximide and WR-1065 on radiation-induced repair processes: A mechanism for chemoprevention. Carcinogenesis 16, 2699-2705 (1995).
43. C. L. Limoli, J. J. Corcoran, J. R. Milligan, J. F. Ward and W. F. Morgan, Critical target and dose and dose-rate responses for the induction of chromosomal instability by ionizing radiation. Radiat. Res. 151, 677-685 (1999).
44. L. L. Ling and J. F. Ward, Radiosensitization of Chinese hamster V79 cells by bromodeoxyuridine substitution of thymidine: Enhancement of radiation-induced toxicity and DNA strand break production by monofilar and bifilar substitution. Radiat. Res. 121, 76-83 (1990).
45. W. F. Morgan, Non-targeted and delayed effects of exposure to ionizing radiation: I. Radiation-induced genomic instability and bystander effects in vitro. Radiat. Res. 159, 567-580 (2003).
46. J. L. Redpath and M. Gutierrez, Kinetics of induction of reactive oxygen species during the post-irradiation expression of neoplastic transformation in vitro. Int. J. Radiat. Biol. 77, 1081-1085 (2001).
47. R. E. Rugo and R. H. Schiestl, Increases in oxidative stress in the progeny of X-irradiated cells. Radiat. Res. 162, 416-425 (2004).
48. C. Laurent, J. P. Pouget and P. Voisin, Modulation of DNA damage by pentoxifylline and alpha-tocopherol in skin fibroblasts exposed to gamma rays. Radiat. Res. 164, 63-72 (2005).
49. J. Carmichael, W. G. DeGraff, A. F. Gazdar, J. D. Minna and J. B. Mitchell, Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Res. 47, 936-942 (1987). (Pubitemid 17027905)