TEV protease-facilitated stoichiometric delivery of multiple genes using a single expression vector

Protein Science

Volumn 19, Issue 12, 2010, Pages 2379-2388

  Chen, Xi ^a   Pham, Elizabeth ^a   Truong, Kevin ^a,b  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

Fusion proteins; Live cell imaging; Polycistronic protein expression; TEV protease

Indexed keywords

HYBRID PROTEIN; PROTEINASE;

ANIMAL CELL; ARTICLE; BACTERIAL CELL; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; FEMALE; GENE EXPRESSION; GENE TARGETING; HUMAN; HUMAN CELL; MAMMAL CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POTYVIRUS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROCESSING; PROTEIN PURIFICATION; RNA TRANSLATION; RNA VIRUS; STOICHIOMETRY; TARGET CELL; TOBACCO VIRUS;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; ESCHERICHIA COLI; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; MULTIGENE FAMILY; RECOMBINANT FUSION PROTEINS;

MAMMALIA; RNA VIRUSES; TOBACCO ETCH VIRUS;

EID: 78649652504     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.518     Document Type: Article

References (42)

1. Szymczak AL, Workman CJ, Wang Y, Vignali KM, Dilioglou S, Vanin EF, Vignali DAA (2004) Correction of multi-gene deficiency in vivo using a single 'selfcleaving' 2A peptide-based retroviral vector. Nat Biotechnol 22:589-594.
2. Bashor CJ, Helman NC, Yan S, Lim WA (2008) Using engineered scaffold interactions to reshape map kinase pathway signaling dynamics. Science 319:1539-1543.
3. Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663-676.
4. Villemure J-F, Savard N, Belmaaza A (2001) Promoter suppression in cultured mammalian cells can be blocked by the chicken b-globin chromatin insulator 5'HS4 and matrix/scaffold attachment regions. J Mol Biol 312:963-974.
5. Chen W-S, Chang Y-C, Chen Y-J, Chen Y-J, Teng C-Y, Wang C-H, Wu T-Y (2009) Development of a prokaryotic- like polycistronic baculovirus expression vector by the linkage of two internal ribosome entry sites. J Virol Methods 159:152-159.
6. Mizuguchi H, Xu Z, Ishii-Watabe A, Uchida E, Hayakawa T (2000) IRES-dependent second gene expression is significantly lower than cap-dependent first gene expression in a bicistronic vector. Mol Ther 1:376-382.
7. Donnelly MLL, Luke G, Mehrotra A, Li X, Hughes LE, Gani D, Ryan MD (2001) Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'. J Gen Virol 82:1013-1025.
8. Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S (2008) Generation of mouse induced pluripotent stem cells without viral vectors. Science 322:949-953.
9. Fang J, Qian J-J, Yi S, Harding TC, Tu GH, VanRoey M, Jooss K (2005) Stable antibody expression at therapeutic levels using the 2A peptide. Nat Biotechnol 23:584-590.
10. Donnelly M, Gani D, Flint M, Monaghan S, Ryan M (1997) The cleavage activities of aphthovirus and cardiovirus 2A proteins. J Gen Virol 78:13-21.
11. de Felipe P, Luke GA, Hughes LE, Gani D, Halpin C, Ryan MD (2006) E unum pluribus: multiple proteins from a self-processing polyprotein. Trends Biotechnol 24:68-75.
12. de Felipe P, Luke GA, Brown JD, Ryan MD (2010) Inhibition of 2A-mediated lsquocleavagersquo of certain artificial polyproteins bearing N-terminal signal sequences. Biotechnol J 5:213-223.
13. de Felipe P, Ryan MD (2004) Targeting of proteins derived from self-processing polyproteins containing multiple signal sequences. Traffic 5:616-626.
14. Dougherty WG, Semler BL (1993) Expression of virus-encoded proteinases: functional and structural similarities with cellular enzymes. Microbiol Rev 57:781-822.
15. Allison R, Johnston RE, Dougherty WG (1986) The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: Evidence for the synthesis of a single polyprotein. Virology 154:9-20.
16. Adams MJ, Antoniw JF, Beaudoin F (2005) Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Mol Plant Pathol 6:471-487.
17. Kapust RB, Tözsér J, Copeland TD, Waugh DS (2002) The P1′ specificity of tobacco etch virus protease. Biochem Biophys Res Commun 294:949-955.
18. Kapust RB, Waugh DS (2000) Controlled intracellular processing of fusion proteins by TEV protease. Protein Expr Purif 19:312-318.
19. Hartmann BM, Kaar W, Yoo IK, Lua LHL, Falconer RJ, Middelberg APJ (2009) The chromatography-free release, isolation and purification of recombinant peptide for fibril self-assembly. Biotechnol Bioeng 104:973-985.
20. Tubb MR, Smith LE, Davidson WS (2009) Purification of recombinant apolipoproteins A-I and A-IV and efficient affinity tag cleavage by tobacco etch virus protease. J Lipid Res 50:1497-1504.
21. Higuchi T, Uhlmann F (2005) Stabilization of microtubule dynamics at anaphase onset promotes chromosome segregation. Nature 433:171-176.
22. Pauli A, Althoff F, Oliveira RA, Heidmann S, Schuldiner O, Lehner CF, Dickson BJ, Nasmyth K (2008) Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons. Dev Cell 14:239-251.
23. Sato M, Toda T (2007) Alp7/TACC is a crucial target in Ran-GTPase-dependent spindle formation in fission yeast. Nature 447:334-337.
24. Harder B, Schomburg A, Pflanz R, Küstner KM, Gerlach N, Schuh R (2008) TEV protease-mediated cleavage in Drosophila as a tool to analyze protein functions in living organisms. Biotechniques 44:765-772.
25. Marcos JF, Beachy RN (1997) Transgenic accumulation of two plant virus coat proteins on a single self processing polypeptide. J Gen Virol 78:1771-1778.
26. Shih Y-P, Wu H-C, Hu S-M, Wang T-F, Wang AH-J (2005) Self-cleavage of fusion protein in vivo using TEV protease to yield native protein. Protein Sci 14:936-941.
27. Kapust RB, Tözsér J, Fox JD, Anderson DE, Cherry S, Copeland TD, Waugh DS (2001) Tobacco etch virus protease: Mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng 14:993-1000.
28. Phan J, Zdanov A, Evdokimov AG, Tropea JE, Peters HK, III, Kapust RB, Li M, Wlodawer A, Waugh DS (2002) Structural basis for the substrate specificity of tobacco etch virus protease. J Biol Chem 277:50564-50572.
29. Rekas A, Alattia J-R, Nagai T, Miyawaki A, Ikura M (2002) Crystal structure of Venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity. J Biol Chem 277:50573-50578.
30. Rizzo MA, Springer GH, Granada B, Piston DW (2004) An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol 22:445-449.
31. Henrichs T, Mikhaleva N, Conz C, Elke D, Boyd D, Zelazny A, Bibi E, Ban N, Ehrmann M (2005) Target-directed proteolysis at the ribosome. Proc Natl Acad Sci USA 102:4246-4251.
32. Köhler F (2003) A yeast-based growth assay for the analysis of site-specific proteases. Nucleic Acids Res 31:e16.
33. Wehr MC, Laage R, Bolz U, Fischer TM, Grunewald S, Scheek S, Bach A, Nave K-A, Rossner MJ (2006) Monitoring regulated protein-protein interactions using split TEV. Nat Methods 3:985-993.
34. Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, Tsien RY (2002) A monomeric red fluorescent protein. Proc Natl Acad Sci USA 99:7877-7882.
35. Tan S, Hunziker Y, Sargent DF, Richmond TJ (1996) Crystal structure of a yeast TFIIA/TBP/DNA complex. Nature 381:127-134.
36. Mizuno T, Okamoto T, Yokoi M, Izumi M, Kobayashi A, Hachiya T, Tamai K, Inoue T, Hanaoka F (1996) Identification of the nuclear localization signal of mouse DNA primase: nuclear transport of p46 subunit is facilitated by interaction with p54 subunit. J Cell Sci 109:2627-2636.
37. Oancea E, Teruel MN, Quest AFG, Meyer T (1998) Green fluorescent protein (GFP)-tagged cysteine-rich domains from protein kinase C as fluorescent indicators for diacylglycerol signaling in living cells. J Cell Biol 140:485-498.
38. Violin JD, Zhang J, Tsien RY, Newton AC (2003) A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol 161:899-909.
39. Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Südhof TC, Wernig M (2010) Direct conversion of fibroblasts to functional neurons by defined factors. Nature 463:1035-1041.
40. Pham E, Chiang J, Li I, Shum W, Truong K (2007) A computational tool for designing FRET protein biosensors by rigid-body sampling of their conformational space. Structure 15:515-523.
41. Chiang J, Li I, Truong K (2006) Creation of circularly permutated yellow fluorescent proteins using fluorescence screening and a tandem fusion template. Biotechnol Lett 28:471-475.
42. Truong K, Khorchid A, Ikura M (2003) A fluorescent cassette-based strategy for engineering multiple domain fusion proteins. BMC Biotechnol 3:8.