ROCK inhibition facilitates the generation of human-induced pluripotent stem cells in a defined, feeder-, and serum-free system

Cellular Reprogramming

Volumn 12, Issue 6, 2010, Pages 641-653

  Lai, Wing Hon ^a   Ho, Jenny Chung Yee ^a   Lee, Yee Ki ^a   Ng, Kwong Man ^a   Au, Ka Wing ^a   Chan, Yau Chi ^a   Lau, Chu Pak ^a   Tse, Hung Fat ^a   Siu, Chung Wah ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; STAGE SPECIFIC EMBRYO ANTIGEN 4; TUMOR REJECTION ANTIGEN;

ADULT; ANIMAL EXPERIMENT; ARTICLE; CELL DIFFERENTIATION; CELL LINE; CELL LINEAGE; CELL TRANSPLANTATION; CONTROLLED STUDY; CULTURE MEDIUM; GERM LAYER; HUMAN; HUMAN CELL; KARYOTYPE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; SERUM; SKIN FIBROBLAST; SOMATIC CELL; TERATOMA;

ADULT; ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE; CULTURE MEDIA, SERUM-FREE; FIBROBLASTS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; KARYOTYPING; MICE; RHO-ASSOCIATED KINASES;

ANIMALIA; MUS;

EID: 78649507841     PISSN: 21524971     EISSN: 21524998     Source Type: Journal    
DOI: 10.1089/cell.2010.0051     Document Type: Article

References (35)

1. Amit, M., Shariki, C., Margulets, V., et al. (2004). Feeder layer-and serum-free culture of human embryonic stem cells. Biol. Reprod. 70, 837-845.
2. Baharvand, H., Salekdeh, G.H., Taei, A., et al. (2010). An efficient and easy-to-use cryopreservation protocol for human ES and iPS cells. Nat. Protoc. 5, 588-594.
3. Braam, S.R., Nauw, R., Ward-van Oostwaard, D., et al. (2010). Inhibition of ROCK improves survival of human embryonic stem cell-derived cardiomyocytes after dissociation. Ann. N. Y. Acad. Sci. 1188, 52-57.
4. Chen, M., Sun, X., Jiang, R., et al. (2009). Role of MEF feeder cells in direct reprogramming of mousetail-tip fibroblasts. Cell. Biol. Int. 33, 1268-1273.
5. Eiselleova, L., Peterkova, I., Neradil, J., et al. (2008). Comparative study of mouse and human feeder cells for human embryonic stem cells. Int. J. Dev. Biol. 52, 353-363.
6. Giorgetti, A., Montserrat, N., Aasen, T., et al. (2009). Generation of induced pluripotent stem cells from human cord blood using OCT4 and SOX2. Cell Stem Cell 5, 353-357.
7. Harb, N., Archer, T. K., and Sato, N. (2008). The Rho-Rock-Myosin signaling axis determines cell-cell integrity of self-renewing pluripotent stem cells. PLoS One 3, e3001.
8. Kim, D., Kim, C.H., Moon, J.I., et al. (2009). Generation of human induced pluripotent stem cells by direct delivery of repro-gramming proteins. Cell Stem Cell 4, 472-476.
9. Klimanskaya, I., Chung, Y., Meisner, L., et al. (2005). Human embryonic stem cells derived without feeder cells. Lancet 365, 1636-1641.
10. Krawetz, R.J., Li, X., and Rancourt, D.E. (2009). Human embryonic stem cells: caught between a ROCK inhibitor and a hard place. Bioessays 31, 336-343.
11. Li, W., Zhou, H., Abujarour, R., et al. (2009a). Generation of human induced pluripotent stem cells in the absence of exogenous Sox2. Stem Cells 27, 2992-3000.
12. Li, X., Krawetz, R., Liu, S., et al. (2009b). ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells. Hum. Reprod. 24, 580-589.
13. Lim, J.W., and Bodnar, A. (2002). Proteome analysis of conditioned medium from mouse embryonic fibroblast feeder layers which support the growth of human embryonic stem cells. Proteomics 2, 1187-1203.
14. Ludwig, T.E., Levenstein, M.E., Jones, J.M., et al. (2006). Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol. 24, 185-187.
15. Martin, M.J., Muotri, A., Gage, F., et al. (2005). Human embryonic stem cells express an immunogenic nonhuman sialic acid. Nat. Med. 11, 228-232.
16. Mollamohammadi, S., Taei, A., Pakzad, M., et al. (2009). A simple and efficient cryopreservation method for feeder-free dissociated human induced pluripotent stem cells and human embryonic stem cells. Hum. Reprod. 24, 2468-2476.
17. Nakagawa, M., Koyanagi, M., Tanabe, K., et al. (2008). Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat. Biotechnol. 26, 101-106.
18. Nemati, S., Hatami, M., Kiani, S., et al. (2010). Long-term self-renewable feeder-free human induced pluripotent stem cell-derived neural progenitors. Stem Cells Dev.
19. Nishikawa, S., Goldstein, R.A., and Nierras, C.R. (2008). The promise of human induced pluripotent stem cells for research and therapy. Nat. Rev. Mol. Cell Biol. 9, 725-729.
20. Pakzad, M., Totonchi, M., Taei, A., et al. (2010). Presence of a ROCK inhibitor in extracellular matrix supports more undif-ferentiated growth of feeder-free human embryonic and induced pluripotent stem cells upon passaging. Stem Cell Rev. 6, 96-107.
21. Park, I.H., Zhao, R., West, J.A., et al. (2008). Reprogramming of human somatic cells to pluripotency with defined factors. Nature 451, 141-146.
22. Peerani, R., Rao, B.M., Bauwens, C., et al. (2007). Niche-mediated control of human embryonic stem cell self-renewal and differentiation. EMBO J. 26, 4744-4755.
23. Soh, B.S., Song, C.M., Vallier, L., et al. (2007). Pleiotrophin enhances clonal growth and long-term expansion of human embryonic stem cells. Stem Cells 25, 3029-3037.
24. Stojkovic, P., Lako, M., Stewart, R., et al. (2005). An autogeneic feeder cell system that efficiently supports growth of undif-ferentiated human embryonic stem cells. Stem Cells 23, 306-314.
25. Takahashi, K., Tanabe, K., Ohnuki, M., et al. (2007). Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872.
26. Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., et al. (1998). Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147.
27. Vasa, M., Fichtlscherer, S., Adler, K., et al. (2001). Increase in circulating endothelial progenitor cells by statin therapy in patients with stable coronary artery disease. Circulation 103, 2885-2890.
28. Watanabe, K., Ueno, M., Kamiya, D., et al. (2007). A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat. Biotechnol. 25, 681-686.
29. Xu, C., Inokuma, M. S., Denham, J., et al. (2001). Feeder-free growth of undifferentiated human embryonic stem cells. Nat. Biotechnol. 19, 971-974.
30. Xu, C., Rosler, E., Jiang, J., et al. (2005a). Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium. Stem Cells 23, 315-323.
31. Xu, R.H., Peck, R.M., Li, D.S., et al. (2005b). Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods 2, 185-190.
32. Yamanaka, S. (2007). Strategies and new developments in the generation of patient-specific pluripotent stem cells. Cell Stem Cell 1, 39-49.
33. Yu, J., Hu, K., Smuga-Otto, K., et al. (2009). Human induced pluripotent stem cells free of vector and transgene sequences. Science 324, 797-801.
34. Yu, J., Vodyanik, M.A., Smuga-Otto, K., et al. (2007). Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917-1920.
35. Zhou, W., and Freed, C.R. (2009). Adenoviral gene delivery can reprogram human fibroblasts to induced pluripotent stem cells. Stem Cells 27, 2667-2674.