Application of an aqueous two-phase systems high-throughput screening method to evaluate mAb HCP separation

Biotechnology and Bioengineering

Volumn 108, Issue 1, 2011, Pages 69-81

  Oelmeier, Stefan A ^a   Dismer, Florian ^a   Hubbuch, Jürgen ^a  

^a KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

Author keywords

Aqueous two phase system; Design of experiments; High throughput screening; Monoclonal antibody; Multilinear regression

Indexed keywords

AQUEOUS TWO PHASE SYSTEM; AUTOMATED APPLICATIONS; AUTOMATED SCREENING; BINODALS; BIOPHARMACEUTICAL SEPARATIONS; BIOTECH INDUSTRY; CAPACITY LIMITATION; CHROMATOGRAPHIC PROCEDURE; CLOUD POINT METHOD; EXPERIMENTAL ERRORS; HIGH-THROUGHPUT SCREENING; HOST CELL PROTEIN; INDUSTRIAL SEPARATION; INFLUENCE OF PH; LABOR INTENSIVE; MODEL PROTEINS; MODEL SYSTEM; MULTI-LINEAR REGRESSION; NACL CONCENTRATION; PARTITION COEFFICIENT; PROTEIN CONCENTRATIONS; SCREENING PROCEDURES; SEPARATION TECHNIQUES; SPEED-UPS; STATISTICAL EVALUATION; TARGET PROTEINS; TIE LINE;

AUTOMATION; DESIGN OF EXPERIMENTS; ENZYMES; LAKES; MERGERS AND ACQUISITIONS; MONOCLONAL ANTIBODIES; SODIUM CHLORIDE; SURFACE PLASMON RESONANCE; THROUGHPUT;

ALLERGIES;

CELL PROTEIN; LYSOZYME; MACROGOL 4000; MONOCLONAL ANTIBODY; OXYGEN; PHOSPHORUS; PROTEIN; SODIUM CHLORIDE;

ARTICLE; AUTOMATION; CHROMATOGRAPHY; CONCENTRATION (PARAMETERS); HIGH THROUGHPUT SCREENING; HOST CELL; INDUSTRY; PH; SCREENING; SEPARATION ANXIETY; SEPARATION TECHNIQUE;

ANTIBODIES, MONOCLONAL; CHEMICAL FRACTIONATION; HIGH-THROUGHPUT SCREENING ASSAYS; POLYETHYLENE GLYCOLS; RECOMBINANT PROTEINS; SOLUTIONS;

EID: 78649424514     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22900     Document Type: Article

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