Purification of a recombinant heavy chain fragment C vaccine candidate against botulinum serotype C neurotoxin [rBoNTC(Hc)] expressed in Pichia pastoris

Protein Expression and Purification

Volumn 75, Issue 2, 2011, Pages 177-185

  Dux, Michael P ^a,e   Huang, Jicai ^b,e   Barent, Rick ^c,e   Inan, Mehmet ^d,e   Swanson, S Todd ^e   Sinha, Jayanta ^e   Ross, John T ^f   Smith, Leonard A ^g   Smith, Theresa J ^h   Henderson, Ian ^f,i   Meagher, Michael M ^e  

^a Novartis Animal Health US Inc   (United States)

^b NEKTAR THERAPEUTICS   (United States)

^c 21401 West Center Road   (United States)

^d Alder Pharmaceuticals   (United States)

^e UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^f A CSC Company   (United States)

^g U S ARMY MEDICAL RESEARCH AND MATERIEL COMMAND   (United States)

^h UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES   (United States)

ⁱ Emergent Product Development Gaithersburg Inc   (United States)

Author keywords

Botulinum toxin vaccine; Pichia pastoris; Protein purification; Recombinant

Indexed keywords

BACTERIAL VACCINE; BOTULINUM TOXIN; BOTULINUM TOXIN TYPE C; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOLOGICAL WARFARE; BIOTECHNOLOGY; CHEMISTRY; CHROMATOGRAPHY; CLOSTRIDIUM BOTULINUM; FEMALE; FERMENTATION; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOUSE; PICHIA; PILOT STUDY; TECHNOLOGY;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL VACCINES; BIOTECHNOLOGY; BIOTERRORISM; BOTULINUM TOXINS; CHROMATOGRAPHY; CLONING, MOLECULAR; CLOSTRIDIUM BOTULINUM; FEMALE; FERMENTATION; MICE; MOLECULAR SEQUENCE DATA; PICHIA; PILOT PROJECTS; RECOMBINANT PROTEINS; TECHNOLOGY TRANSFER;

PICHIA PASTORIS;

EID: 78649331561     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.09.016     Document Type: Article

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