Scale-up of the fermentation and purification of the recombinant heavy chain fragment C of botulinum neurotoxin serotype F, expressed in Pichia pastoris

Protein Expression and Purification

Volumn 32, Issue 1, 2003, Pages 1-9

  Johnson, Scott K ^a   Zhang, Wenhui ^a   Smith, Leonard A ^b   Hywood Potter, Karen J ^a   Swanson, S Todd ^a   Schlegel, Vicki L ^a   Meagher, Michael M ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^b UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PICHIA; PICHIA PASTORIS;

EID: 0344099420     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2003.07.003     Document Type: Article

References (30)

1. C. Lamanna, E.R. Hart, Relationship of lethal toxic dose to body weight of the mouse, Toxicol. Appl. Pharmacol. 13 (1968) 307-315.
2. J.H. Anderson, G.E. Lewis, Clinical evaluation of botulinum toxoids, in: G.E. Lewis (Ed.), Biomedical Aspects of Botulism, Academic Press, New York, 1981, pp. 233-246.
3. E. Habermann, F. Dreyer, Clostridial neurotoxins: handling and action at the cellular and molecular level, Top. Microbiolog. Immunol. 129 (1986) 93-179.
4. G. Schiavo, F. Benfenati, B. Poulain, O. Rossetto, P. Polverino, de Laureto, B.R. Dasgupta, C. Montecucco, Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin, Nature 359 (1992) 832-835.
5. L.L. Simpson, Molecular pharmacology of botulinum toxin and tetanus toxin, Annu. Rev. Pharmacol. Toxicol. 26 (1986) 427-453.
6. B.R. DasGupta, The structure of botulinum neurotoxins, in: L.L. Simpson (Ed.), Botulinum Neurotoxin and Tetanus Toxin, Academic Press, New York, 1989, pp. 53-67.
7. L.L. Simpson, The origin, structure, and pharmacological activity of botulinum toxin.", Pharmacol. Rev. 33 (1981) 155-188.
8. B. Poulain, U. Weller, T. Binz, H. Neimann, B. De Pavia, O. Dolly, C. Leprince, L. Tauc, Functional roles of the domains of clostridial neurotoxins: the contribution from studies on Aplysia, in: B.R. DasGupta (Ed.), Botulinum and Tenanus Neurontoxins: Neurotransmission and Biomedical Aspects. Plenum Press, New York, 1993, pp. 34-360.
9. 125I-labeled botulinum neurotoxin with nerve terminals. I. Ultrastructual autoradiographic localization and quantification of distinct membrane acceptors for types A and B on motor nerves, J. Cell Biol. 103 (1986) 521-534.
10. T.-I. Nishiki, Y. Kamata, Y. Nemoto, A. Omori, T. Ito, M. Takahashi, S. Kozaki, Identification of protein receptor for Clostridium botulinum type B neurotoxin in rat brain synaptosomes, J. Biol. Chem. 269 (1994) 10498-10503.
11. C.C. Shone, P. Hambleton, J. Melling, Inactivation of Clostridium botulinum type A neurotoxin by trypsin and purification of two tryptic fragments. Proteolytic cleavage near the COOH-terminus of the heavy subunit destroys toxin-binding activity, Eur. J. Biochem. 151 (1985) 75-82.
12. G. Schiavo, O. Rossetto, S. Catsicas, P. Poverino, de Laureto, B.R. Dasgupta, F. Benfenati, C. Montecucco, Identification of the nerve terminal targets of botulinum neurotoxin serotypes A, D and E, E.J. Biol. Chem. 268 (1993) 23784-23787.
13. 125I-labeled botulinum neurotoxin with nerve terminals. II. Autoradiographic evidence for its uptake into motor nerves by acceptor-mediated endocytosis, J. Cell Biol. 103 (1986) 535-544.
14. 2-terminus of the heavy subunit of Clostridium botulinum type A neurotoxin forms channels in lipid vesicles, Eur. J. Biochem. 167 (1987) 175-180.
15. R.O. Blaustein, W.J. Germann, A. Finkelstein, B.R. Dasgupta, The N-terminal half of the heavy chain of botulinum type A neurotoxin forms channels in planar phospholipid bilayers, FEBS Lett. 226 (1987) 115-120.
16. M.S. Montal, R. Blewitt, J.M. Tomich, M. Montal, Identification of an ion channel-forming motif in the primary structure of tetanus and botulinum neurotoxins, FEBS Lett. 313 (1992) 12-18.
17. G. Schiavo, O. Rossetto, F. Benfenati, B. Poulin, C. Montecucco, Tetanus and botulinum neurotoxins are zinc proteases specific for components of the neuroexocytosis Appartus, Annu. N. Y. Acad. Sci. 710 (1994) 65-75.
18. S. Tonello, S. Morante, O. Rossetto, G. Schiavo, C. Montecucco, Tetanus and botulinum neurotoxins, a novel group of zinc-endopeptisadases, in: K. Suziki, J. Bond (Eds.), Intracelluar Protein Catabolism, Plenum Press, New York, 1996, pp. 251-260.
19. G. Schiavo, B. Poulain, O. Rossetto, F. Benfenati, L. Tauc, C. Montecucco, Tetanus toxin is a zinc protein and its inhibition of neurotransmitter release and protease activity depends on Zinc, EMBO J. 11 (1992) 3577-3583.
20. C.C. Shone, C.P. Quinn, R. Wait, B. Hallis, S. Fooks, P. Hambleton, Proteolytic cleavage of synthetic fragments of vesicle-associated membrane protein, isoform-2 by botulinum type B neurotoxin, Eur. J. Biochem. 217 (1993) 965-971.
21. L.A. Smith, M. Byrne, Vaccines for preventing botulism, in: M. Brin, J. Jankovic, Hallett (Eds.), Scientific and Therapeutic Aspects of Botulinum Toxin, Lippincott, Williams & Wilkens, Philadelphia, 2002, pp. 427-440.
22. M.A. Clayton, J.M. Clayton, D.R. Brown, J.L. Middlebrook, Protective vaccination with a recombinant fragment of Clostridium botulinum neurotoxin serotype A expressed from a synthetic gene in Escherichia coli, Infection and Immunity 63 (1995) 2738-2742.
23. M.P. Byrne, R.W. Titball, J. Holley, L.A. Smith, Fermentation, purification and efficacy of a recombinant vaccine candidate against botulinum neurotoxin type F from Pichia pastoris, Prot. Exp. Purif. 18 (2000) 327-337.
24. L.A. Smith, Development of recombinant vaccines for botulinum neurotoxin, Toxicon. 36 (1998) 1539-1548.
25. C.L. Hathaway, Toxoid of Clostridium botulinum type F: purification and immunogenicity studies, Appl. Environ. Microbiol. 31 (1976) 234-242.
26. K.J. Potter, M.A. Bevins, E.V. Vassilieva, V.R. Chiruvolu, T. Smith, L.A. Smith, M.M. Meagher, Production and purification of the heavy-chain fragment C of botulinum neurotoxin, serotype B, expressed in the methylotrophic yeast Pichia pastoris, Prot. Exp. Purif. 13 (1998) 357-365.
27. K.J. Potter, W. Zhang, L.A Smith, M.M. Meagher, Production and purification of the heavy chain fragment C of botulinum neurotoxin, serotype A, expressed in the methylotrophic yeast Pichia pastoris, Prot. Exp. Purif. 19 (2000) 393-402.
28. T. Kubota, T. Watanabe, N. Yokosawa, K. Tsuzuki, T. Indoh, K. Moriishi, K. Sanda, Y. Maki, K. Inoue, N. Fujii, Epitope regions in the heavy chain of Clostridium botulinum type E neurotoxin recognized by monoclonal antibodies, Appl. Environ. Microbiol. 63 (1997) 1214-1218.
29. V. Chiruvolu, J.M. Cregg, M.M. Meagher, Recombinant protein expression in an alcohol oxidase-defective strain of Pichia pastoris in feed-batch fermentations, Enzyme Microbiol. Technol. 21 (1997) 277-283.
30. F. Sherman, R.P. Moerschell, S. Tsunasawa, R. Sternglanz, in: K. Imahori, F. Sakiyama (Eds.), Methods in Protein Sequence analysis, Plenum, New York, 1993, pp. 173-181.