Analysis of mRNA from human heart tissue and putative applications in forensic molecular pathology

Forensic Science International

Volumn 203, Issue 1-3, 2010, Pages 99-105

  Partemi, Sara ^a   Berne, Paola M ^b   Batlle, Montserrat ^b   Berruezo, Antonio ^b   Mont, Luis ^b   Riuró, Helena ^c   Ortiz, José T ^b   Roig, Eulalia ^b   Pascali, Vincenzo L ^a   Brugada, Ramon ^c   Brugada, Josep ^b   Oliva, Antonio ^a  

^a CATHOLIC UNIVERSITY   (Italy)

^b UNIVERSITY OF BARCELONA   (Spain)

^c UNIVERSITY OF GIRONA   (Spain)

Author keywords

Cardiovascular diseases; Degradation; MRNA quantification; Post mortem interval; Stability

Indexed keywords

BETA GLUCURONIDASE; COLLAGEN; COLLAGEN TYPE 1A1; COLLAGEN TYPE 3A1; COMPLEMENTARY DNA; ENDOTHELIAL NITRIC OXIDE SYNTHASE; MESSENGER RNA; RNA; UNCLASSIFIED DRUG;

ARTICLE; AUTOPSY; CARDIOVASCULAR DISEASE; CAUSE OF DEATH; CONTROLLED STUDY; FORENSIC PATHOLOGY; GENE EXPRESSION; HEART; HEART MUSCLE ISCHEMIA; HEART RIGHT VENTRICLE; HUMAN; HUMAN TISSUE; MOLECULAR BIOLOGY; NUCLEOTIDE SEQUENCE; ORGAN DONOR; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; RNA ANALYSIS; RNA DEGRADATION; RNA ISOLATION; RNA STABILITY;

EID: 78649318693     PISSN: 03790738     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.forsciint.2010.07.005     Document Type: Article

References (29)

1. Bauer M., Gramlich I., Polzin S., Patzelt D. Quantification of mRNA degradation as possible indicator of postmortem interval-a pilot study. Leg. Med. (Tokyo) 2003, 5(4):220-227.
2. Anderson S., Howard B., Hobbs G.R., Bishop C.P. A method for determining the age of a bloodstain. Forensic Sci. Int. 2005, 148:37-45.
3. Bauer M., Polzin S., Patzelt D. Quantification of RNA degradation by semi-quantitative duplex and competitive RT-PCR: a possible indicator of the age of bloodstains. Forensic Sci. Int. 2003, 138:94-103.
4. Bauer M., Kraus A., Patzelt D. Detection of epithelial cells in dried blood stains by reverse transcriptase-polymerase chain reaction. J. Forensic Sci. 1999, 44:1232-1236.
5. Ohshima T., Sato Y. Time-dependent expression of interleukin-10 (IL-10) mRNA during the early phase of skin wound healing as a possible indicator of wound vitality. Int. J. Legal Med. 1998, 111:251-255.
6. Zhao D., Ishikawa T., Quan L., Michiue T., Zhu B.L., Maeda H. Postmortem quantitative mRNA analyses of death investigation in forensic pathology: an overview and prospects. Leg. Med. 2009 April, 11(Suppl. 1):S43-S45.
7. Zhao D., Zhu B.L., Ishikawa T., Quan L., Li D.R., Maeda H. Real-time RT-PCR quantitative assays and postmortem degradation profiles of erythropoietin, vascular endothelial growth factor and hypoxia-inducible factor 1 alpha mRNA transcripts in forensic autopsy materials. Leg. Med. 2006, 8:132-136.
8. Harrison P.J., Heath P.R., Eastwood S.L., Burnet P.W., McDonald B., Pearson R.C. The relative importance of premortem acidosis and postmortem interval for human brain gene expression studies: selective mRNA vulnerability and comparison with their encoded proteins. Neurosci. Lett. 1995, 300:151-154.
9. Li J.Z., Vawter M.P., Walsh D.M., Tomita H., Evans S.J., Choudary P.V., et al. Systematic changes in gene expression in postmortem human brains associated with tissue pH and terminal medical conditions. Hum. Mol. Genet. 2004, 13:609-616.
10. Preece P., Cairns N.J. Quantifying mRNA in postmortem human brain: influence of gender, age at death, postmortem interval, brain pH, agonal state and inter-lobe mRNA variance. Mol. Brain Res. 2003, 118:60-71.
11. Lin J., Kawano H., Paparella M.M., Ho S.B. Improved RNA analysis for immediate autopsy of temporal bone soft tissues. Acta Octolaryngol. 1999, 119:421-426.
12. Yasojima K., McGeer E.G., McGeer P.L. High stability of mRNAs postmortem and protocols for their assessment by RT-PCR. Brain Res. Protoc. 2001, 8:212-218.
13. De Paepe M.E., Mao Q., Huang C., Zhu D., Jackson C.L., Hansen K. Postmortem RNA and protein stability in perinatal human lungs. Diagn. Mol. Pathol. 2002, 11:170-176.
14. Kuliwaba J.S., Fazzalari N.L., Findlay D.M. Stability of RNA isolated from human trabecular bone at post-mortem and surgery. Biochim. Biophys. Acta 2005, 1740:1-11.
15. Malik K.J., Chen C.D., Olsen T.W. Stability of RNA from the retina and retinal pigment epithelium in a porcine model simulating human eye bank conditions. Invest. Opthlamol. Vis. Sci. 2003, 44:2730-2735.
16. de Moreau de Gerbehaye A.I., Bodeus M., Robert A., Horsmans Y., Goubau P. Stable hepatitis C virus RNA detection by RT-PCR during four days storage. BMC Infect. Dis. 2002, 2:22.
17. Kessler H.H., Stelzl E., Raggam R.B., Haas J., Kirchmeir F., Hegenbarth K., et al. Effects of storage and type of blood collection tubes on hepatitis C virus level in whole blood samples. J. Clin. Microbiol. 2001, 39:1788-1790.
18. Heinrich M., Matt K., Lutz-Bonengel S., Schmidt U. Successful RNA extraction from various human postmortem tissues. Int. J. Legal Med. 2007, 121:136-142.
19. Barton A.J., Pearson R.C., Najlerahim A., Harrison P.J. Pre- and postmortem influences on brain RNA. J. Neurochem. 1993, 61:1-11.
20. Tomita H., Vawter M.P., Walsh D.M., Evans S.J., Choudary P.V., Li J., et al. Effect of agonal and postmortem factors on gene expression profile: quality control in microarray analyses of postmortem human brain. Biol. Psychiatry 2004, 55:346-352.
21. Weis S., Llenos I.C., Dulay J.R., Elashoff M., Martinez-Murillo F., Miller C.L. Quality control for microarray analysis of human brain samples: the impact of postmortem factors, RNA characteristics, and histopathology. J. Neurosci. Methods 2007, 165:198-209.
22. Ausubel F.M., Brkremddsjgsjask Current Protocols in Molecular Biology 2009, vol. 1. John Wiley & Sons Inc., (Chapter 4).
23. Sambrook J., Fefmt Molecular Cloning: A Laboratory Manual 2009, (book 3). 2nd ed.
24. Weber K.T. Cardiac interstitium in health and disease: the fibrillar collagen network. J. Am. Coll. Cardiol. 1989, 13:1637-1652.
25. Fish J.E., Matouk C.C., Yeboah E., Bevan S.C., Khan M., Patil K., et al. Hypoxia-inducible expression of a natural cis-antisense transcript inhibits endothelial nitric-oxide synthase. J. Biol. Chem. 2007, 282:15652-15666.
26. Hauton D., Ousley V. Prenatal hypoxia induces increased cardiac contractility on a background of decreased capillary density. BMC Cardiovasc. Disord. 2009, 9:1.
27. Searles C.D Transcriptional and posttranscriptional regulation of endothelial nitric oxide synthase expression. Am. J. Physiol. Cell Physiol. 2006, 291:803-816.
28. Takemoto M., Sun J., Hiroki J., Shimokawa H., Liao J.K. Rho-kinase mediates hypoxia-induced downregulation of endothelial nitric oxide synthase. Circulation 2002, 106:57-62.
29. Simkhovich B.Z., Marjoram P., Poizat C., Kedes L., Kloner R.A. Brief episode of ischemia activates protective genetic program in rat heart: a gene chip study. Cardiovasc. Res. 2003, 59:450-459.