Successful RNA extraction from various human postmortem tissues

International Journal of Legal Medicine

Volumn 121, Issue 2, 2007, Pages 136-142

  Heinrich, Marielle ^a   Matt, Katja ^a   Lutz Bonengel, Sabine ^a   Schmidt, Ulrike ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Degradation; GAPDH; Human tissue; mRNA; Quantitative real time PCR

Indexed keywords

ARTICLE; AUTOPSY; BRAIN; CAUSE OF DEATH; GENE AMPLIFICATION; GENE EXPRESSION; HEART; HUMAN; PATHOLOGY; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RNA DEGRADATION; RNA EXTRACTION; SKELETAL MUSCLE; TISSUE SLICE;

AUTOPSY; CAUSE OF DEATH; GENE EXPRESSION PROFILING; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; POLYMERASE CHAIN REACTION; POSTMORTEM CHANGES; REPRODUCIBILITY OF RESULTS; RNA STABILITY; RNA, MESSENGER; TIME FACTORS; TISSUE DISTRIBUTION;

EID: 33846931145     PISSN: 09379827     EISSN: 14371596     Source Type: Journal    
DOI: 10.1007/s00414-006-0131-9     Document Type: Article

References (23)

1. Wilusz CJ, Wormington M, Peltz SW (2001) The cap-to-tail guide to mRNA turnover. Nat Rev Mol Cell Biol 2:237-246
2. Catts VS, Catts SV, Fernandez HR, Taylor JM, Coulson EJ, Lutze-Mann LH (2005) A microarray study of post-mortem mRNA degradation in mouse brain tissue. Brain Res Mol Brain Res 138:164-177
3. Preece P, Cairns NJ (2003) Quantifying mRNA in postmortem human brain: influence of gender, age at death, postmortem interval, brain pH, agonal state and inter-lobe mRNA variance. Brain Res Mol Brain Res 118:60-71
4. Fitzpatrick R, Casey OM, Morris D et al (2002) Postmortem stability of RNA isolated from bovine reproductive tissues. Biochim Biophys Acta 1574:10-14
5. Kobayashi H, Sakimura K, Kuwano R et al (1990) Stability of messenger RNA in postmortem human brains and construction of human brain cDNA libraries. J Mol Neurosci 2:29-34
6. Reddy PH, McWeeney S, Park BS et al (2004) Gene expression profiles of transcripts in amyloid precursor protein transgenic mice: upregulation of mitochondrial metabolism and apoptotic genes is an early cellular change in Alzheimer's disease. Hum Mol Genet 13:1225-1240
7. Preece P, Virley DJ, Costandi M et al (2003) An optimistic view for quantifying mRNA in post-mortem human brain. Brain Res Mol Brain Res 116:7-16
8. Bauer M, Polzin S, Patzelt D (2003) Quantification of RNA degradation by semi-quantitative duplex and competitive RT-PCR: a possible indicator of the age of bloodstains? Forensic Sci Int 138:94-103
9. Bauer M, Gramlich I, Polzin S, Patzelt D (2003) Quantification of mRNA degradation as possible indicator of postmortem interval - a pilot study. Leg Med (Tokyo) 5:220-227
10. Bauer M, Patzelt D (2003) A method for simultaneous RNA and DNA isolation from dried blood and semen stains. Forensic Sci Int 136:76-78
11. Loddenkötter B, Becker K, Hohoff C, Brinkmann B, Bajanowski T (2005) Real-time quantitative PCR assay for the detection of Helicobacter pylori: no association with sudden infant death syndrome. Int J Legal Med 119:202-206
12. Bustin SA (2002) Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol 29:23-39
13. Bustin SA (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169-193
14. Goidin D, Mamessier A, Staquet MJ, Schmitt D, Berthier-Vergnes O (2001) Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and beta-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulations. Anal Biochem 295:17-21
15. Aerts JL, Gonzales MI, Topalian SL (2004) Selection of appropriate control genes to assess expression of tumor antigens using real-time RT-PCR. Biotechniques 36:84-86, 88, 90-91
16. Phang TW, Shi CY, Chia JN, Ong CN (1994) Amplification of cDNA via RT-PCR using RNA extracted from postmortem tissues. J Forensic Sci 39:1275-1279
17. Mall G, Eckl M, Sinicina I, Peschel O, Hubig M (2005) Temperature-based death time estimation with only partially known environmental conditions. Int J Legal Med 119:185-194
18. Henssge C, Madea B (2004) Estimation of the time since death in the early post-mortem period. Forensic Sci Int 144:167-175
19. Takamiya M, Saigusa K, Kumagai R, Nakayashiki N, Aoki Y (2005) Studies on mRNA expression of tissue-type plasminogen activator in bruises for wound age estimation. Int J Legal Med 119:16-21
20. Hayashi T, Ishida Y, Kimura A, Takayasu T, Eisenmenger W, Kondo T (2004) Forensic application of VEGF expression to skin wound age determination. Int J Legal Med 118:320-325
21. Suay L, Salvador ML, Abesha E, Klein U (2005) Specific roles of 5′ RNA secondary structures in stabilizing transcripts in chloroplasts. Nucleic Acids Res 33:4754-4761
22. Ikematsu K, Tsuda R, Nakasono I (2006) Gene response of mouse skin to pressure injury in the neck region. Leg Med (Tokyo) 8:128-131
23. Liu J, Lewohl JM, Dodd PR, Randall PK, Harris RA, Mayfield RD (2004) Gene expression profiling of individual cases reveals consistent transcriptional changes in alcoholic human brain. J Neurochem 90:1050-1058