Production and titration of lentiviral vectors

Current Protocols in Neuroscience

Volumn , Issue SUPPL.53, 2010, Pages

  Barde, Isabelle ^a   Salmon, Patrick ^b   Trono, Didier ^a  

^a EPFL   (Switzerland)

^b UNIVERSITY OF GENEVA   (Switzerland)

Author keywords

gene expression; gene transfer; lentivector; lentivirus vector; molecular biology; molecular neurobiology; neuroscience; protein expression; RNA; viral RNAs

Indexed keywords

ANTIGEN P24; BUFFER; CALCIUM CHLORIDE; LENTIVIRUS VECTOR; PLASMID DNA;

293T CELL; ASEPSIS; BIOSAFETY; BIOTECHNOLOGICAL PRODUCTION; CALCULATION; CELL LINE; CENTRIFUGATION; CULTURE MEDIUM; DILUTION; FILTER; FLOW CYTOMETRY; GENE AMPLIFICATION; HUMAN; HUMAN CELL; INCUBATION TEMPERATURE; LAMINAR FLOW; LIFE CYCLE; LONG TERMINAL REPEAT; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL; PSEUDOTYPING; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; REVIEW; TIME; TRANSGENE; TRANSIENT TRANSFECTION; VIRUS CULTURE; VIRUS INFECTIVITY; VIRUS PARTICLE; VIRUS TITRATION;

ANIMALS; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HEK293 CELLS; HIV-1; HUMANS; LENTIVIRUS; TRANSFECTION;

EID: 78449267616     PISSN: 19348584     EISSN: 19348576     Source Type: Journal    
DOI: 10.1002/0471142301.ns0100s37     Document Type: Review

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