A real-time qPCR assay for the detection of the nifH gene of Methanobrevibacter smithii, a potential indicator of sewage pollution

Journal of Applied Microbiology

Volumn 109, Issue 6, 2010, Pages 1946-1956

  Johnston, C ^a,b   Ufnar, J A ^c   Griffith, J F ^d   Gooch, J A ^b   Stewart, J R ^b,e  

^a Jardon and Howard Technologies Inc   (United States)

^b CENTER FOR COASTAL ENVIRONMENTAL HEALTH AND BIOMOLECULAR RESEARCH   (United States)

^c VANDERBILT UNIVERSITY   (United States)

^d SOUTHERN CALIFORNIA COASTAL WATER RESEARCH PROJECT   (United States)

^e University of North Carolina at Chapel Hill   (United States)

Author keywords

Environmental recreational water; Indicators; Intestinal microbiology; Polymerase chain reaction (PCR); Water quality

Indexed keywords

FINANCE; GENES; HEALTH RISKS; SEWAGE; WATER POLLUTION; WATER QUALITY;

ENVIRONMENTAL/RECREATIONAL WATER; INTERNAL CONTROLS; INTESTINAL MICROBIOLOGY; NIFH GENES; POLYMERASE CHAIN REACTION; POTENTIAL INDICATORS; REAL TIME QPCR; RECREATIONAL WATER; SEWAGE POLLUTIONS; WATER SAMPLES;

POLYMERASE CHAIN REACTION;

BIOINDICATOR; DETECTION METHOD; GENE; GENOME; HEALTH RISK; HOST SPECIFICITY; INHIBITION; POLYMERASE CHAIN REACTION; PROKARYOTE; QUANTITATIVE ANALYSIS; REAL TIME; SEWAGE; WATER QUALITY;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; GENE TARGETING; HEALTH HAZARD; HOST RANGE; INDICATOR ORGANISM; INTESTINE FLORA; METHANOBREVIBACTER SMITHII; NIFH GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLLUTION; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SEWAGE; STANDARD; WATER QUALITY; WATER SAMPLING;

ARCHAEA; METHANOBREVIBACTER SMITHII;

EID: 78449237643     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2010.04824.x     Document Type: Article

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