Rapid one-step quantitative reverse transcriptase PCR assay with competitive internal positive control for detection of enteroviruses in environmental samples

Applied and Environmental Microbiology

Volumn 72, Issue 6, 2006, Pages 3960-3967

  Gregory, Jason B ^a,c   Litaker, R Wayne ^b   Noble, Rachel T ^a  

^a University of North Carolina at Chapel Hill   (United States)

^b NOAA Center for Coastal Fisheries and Habitat Research   (United States)

^c CENTER FOR COASTAL ENVIRONMENTAL HEALTH AND BIOMOLECULAR RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; CONTAMINATION; ENZYME INHIBITION; GENES; HEALTH RISKS; WATER;

ENVIRONMENTAL SAMPLES; FECAL CONTAMINATION; HUMAN ENTEROVIRUSES; SHELLFISH HARVESTING WATERS;

VIRUSES;

ANTHROPOGENIC SOURCE; BIOASSAY; BIOINDICATOR; DETECTION METHOD; FECAL COLIFORM; POLLUTION MONITORING; QUANTITATIVE ANALYSIS; RISK ASSESSMENT; VIRUS; WATER POLLUTION; WATER QUALITY;

ARTICLE; COMPETITIVE INTERNAL POSITIVE CONTROL; CONTROL SYSTEM; CONTROLLED STUDY; DILUTION; ENTEROVIRUS; FALSE NEGATIVE RESULT; FECES; HEALTH HAZARD; INFECTION RISK; NONHUMAN; PUBLIC HEALTH SERVICE; QUANTITATIVE ASSAY; RELIABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SHELLFISH; VIRUS CULTURE; VIRUS DETECTION; VIRUS GENOME; WATER CONTAMINATION;

BASE SEQUENCE; DNA PRIMERS; ENTEROVIRUS; ENVIRONMENTAL MONITORING; ENVIRONMENTAL POLLUTION; FECES; GENOME, VIRAL; HUMANS; PUBLIC HEALTH; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; UNITED STATES;

ENTEROVIRUS;

EID: 33745143946     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02291-05     Document Type: Article

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