Development of a plasmid vector for overproduction of β-galactosidase in Escherichia coli by using genetic components of groEx from symbiotic bacteria in Amoeba proteus

Journal of Microbiology and Biotechnology

Volumn 8, Issue 5, 1998, Pages 509-516

  Lee, Jung Eun ^a   Ahn, Eun Young ^a   Ahn, Tae In ^a  

^a Seoul National University   (South Korea)

Author keywords

groE; Heat shock; Promoter; Protein expression; Purification; Transcription terminator

Indexed keywords

AMMONIUM SULFATE; BETA GALACTOSIDASE; HEAT SHOCK PROTEIN; PLASMID VECTOR;

AMOEBA PROTEUS; ARTICLE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; GEL FILTRATION; LACTOSE OPERON; NONHUMAN; PRECIPITATION; PROMOTER REGION; STRUCTURAL GENE; SYMBIOSIS;

ACRONICTA LEPORINA; AMOEBA PROTEUS; AMOEBA PROTEUS SYMBIOTIC BACTERIUM; ESCHERICHIA COLI;

EID: 7844223623     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Ahn, T. I., H. K. Leeu, I. H. Kwak, and K. W. Jeon. 1991. Nucleotide sequence and temperature-dependent expression of xgroEL gene isolated from symbiotic bacteria of Amoeba proteus. Endocyt. Cell Res. 8: 33-44.
2. Ahn, T. I., S. T. Lim, H. K. Leeu, J. E. Lee, and K. W. Jeon. 1994. A novel strong promoter of the groEx operon of symbiotic bacteria in Amoeba proteus. Gene 128: 43-49.
3. Alifano, P., F. Rivellini, D. Limauro, C. B. Bruni, and S. Carlomagno. 1991. A consensus motif common to all rho-dependent prokaryotic transcription terminators. Cell 64: 553-563.
4. Bennett, G. N., M. E. Schweingruber, K. D. Brown, C. Squires, and C. Yanofsky. 1976. Nucleotide sequence of region preceding trp mRNA initiation site and its role in promoter and operator function. Proc. Natl. Acad. Sci. USA 73: 2351-2355.
5. Bentley, W. E., N. Morkajalili, D. D. anderson, R. H. Davis, and D. S. Kompala. 1990. Plasmid-encoded protein: The principle factor in the metabolic burden associated with recombinant bacteria. Biotechnol. Bioeng. 35: 668-681.
6. de Boer, H. A., L. J. Costock, and M. Vasser. 1983. The tac promoter: A functional hybrid derived from the trp and lac promoters. Proc. Natl. Acad. Sci. USA 80: 21-25.
7. Borck, J., J. D. Beggs, W. J. Brammer, A. S. Hoplans, and N. E. Murray. 1976. The construction in vitro of transducing derivatives of phage lambda. Mol Gen. Genet. 146: 199-207.
8. Bullock, W. O., J. M. Fernandez, and J. M. Short. 1987. XL1 Blue: A high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection. Biotechniques 4: 376-379.
9. Cane, D. E., Z. Wu, J. S. Oliver, and T. M. Hohn. 1993. Overproduction of soluble trichodiene synthetase from Fusarium sporotrichioides in Escherichia coli. Arch. Biochem. Biophys. 300: 416-422.
10. Casadeban, M. J. 1976. Transposition and fusion of lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J. Mol. Biol. 104: 541-555.
11. Casadeban, M. J., J. Chou, and S. M. Cohen. 1980. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino terminal fragment of exogenous proteins: Escherichia coli plasmid vectors for detection and cloning of translational initiation signals. J. Bacteriol. 143: 971-980.
12. Cheetham, P. S. J. 1994. Case studies in applied biocatalysis - from ideas to products. pp. 47-108. In J. M. S. Cabrai, D. Best, L. Boross, and J. Tramper (eds.), Applied Biocatalysis. Harwood Academic Pub., Switzerland.
13. Chen, J.-D. and D. A. Morrison. 1987. Cloning of Streptococcus pneumoniae DNA fragments in Escherichia coli requires vectors protected by strong transcriptional terminators. Gene 55: 179-187.
14. Chung, C. T., S. L. Niemela, and R. H. Miller. 1989. One step preparation of competent Escherichia coli: Transformation and storage of bacterial cells in the same solution. Proc. Natl. Acad. Sci. USA 86: 2172-2175.
15. + to the ARE. Mol Cell. Biol. 15: 6331-6340.
16. 4(5α)-dehydrogenase: Gene and protein characterization. J. Bacteriol. 178: 3322-3330.
17. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166: 557-580.
18. Hediger, M. A., D. F. Johnson, D. P. Nierlich, and I. Zabin. 1985. DNA sequence of the lactose operon: The lacA gene and the transcriptional termination region. Proc. Natl. Acad. Sci. USA 82: 6414-6418.
19. Koraimann, G., T. Teferle, R. Mittergger, S. Wangner, and G. Hogenauer. 1996. Differential mRNA decay within the transfer operon of plasmid R1: Identification and analysis of an intracistronic mRNA stabilizer. Mol. Gen. Genet. 250: 466-476.
20. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature 227: 680-685.
21. Lee, J., M. H. Cho, and J. Lee. 1996. Characterization of an oxygen-dependent inducible promoter system, the nar promoter, and Escherichia coli with an inactivated nar operon. Biotechnol. Bioeng. 52: 572-578.
22. Lee, J. E., J. S. Hwang, and T. I. Ahn. 1994. Developing a temperature sensitive expression vector by application of groEx of symbiotic bacteria in Amoeba proteus. Kor. J. Microbiol. 32: 501-509.
23. Lee, J. E., S. T. Lim, and T. I. Ahn. 1996. Characterization of promoter sequences for strong expression of groEx in Escherichia coli. J. Microbiol. 34: 15-22.
24. Lee, S. and S. Rasheed. 1990. A simple procedure for maximum yield of high-quality plasmid DNA. Biotechnique 9: 676-679.
25. Lowry O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275.
26. Olins, P. O. and S. H. Rangwala. 1989. A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli. J. Biol. Chem. 264: 16973-16976.
27. Pestka, S. 1990. Production and analysis of proteins by recombinant DNA technology, pp. 235-265. In S. Stein (ed.), Fundamentals of Protein Biotechnology. Marcel Dekker, Inc., New York, U.S.A.
28. Pirrota, V. 1975. Sequence of the OR operator of phage lambda. Nature 254: 114-117.
29. Platt, T. 1972. Purification of β-galactosidase. pp. 398-404. In J. H. Miller. Experiments in Molecular Genetics. Cold Spring Harbor Lab. Press, Cold Spring Harbor, New York, U.S.A.
30. Platt, T. 1986. Transcription termination and regulation of gene expression. Annu. Rev. Biochem. 55: 339-372.
31. Richardson, J. P. 1991. Preventing the synthesis of unused transcripts by rho factor. Cell 64: 1047-1049.
32. Shapira, S. K., J. Chou, F. V. Richaud, and M. J. Casadaban. 1983. New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding and enzymatically active carboxyterminal portion of beta-galactosidase. Gene 25: 71-82.
33. Studier, F. W. and B. A. Moffatt. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189: 113-130.
34. Yanisch-Perron, C, J. Vierira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119.