Characterization of an oxygen-dependent inducible promoter system, the nar promoter, and Escherichia coli with an inactivated nar operon

Biotechnology and Bioengineering

Volumn 52, Issue 5, 1996, Pages 572-578

  Lee, Jintae ^a   Cho, Moo Hwan ^a   Lee, Jongwon ^b  

^a Yeungnam University   (South Korea)

^b Catholic University of Daegu   (South Korea)

Author keywords

anaerobic conditions; Escherichia coli; inducible promoter; nar promoter; nitrate reductase

Indexed keywords

BACTERIA; BIOASSAY; CHARACTERIZATION; CHROMOSOMES; ENZYMES; FERMENTATION; GENES; MOLYBDENUM COMPOUNDS; NITRATES; OXYGEN; PROTEINS;

ESCHERICHIA COLI; GALACTOSIDASE; INACTIVATED NAR OPERON; INDUCIBLE PROMOTER; INDUCTION RATIO; MOLYBDATE; NAR PROMOTER; NITRATE REDUCTASE;

BIOTECHNOLOGY;

ARTICLE; BACTERIAL GENETICS; BACTERIAL GROWTH; CULTURE MEDIUM; ESCHERICHIA COLI; GENE INDUCTION; NONHUMAN; OPERON; PROMOTER REGION;

EID: 10344261469     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19961205)52:5<572::AID-BIT4>3.0.CO;2-G     Document Type: Article

References (22)

1. Brosius, J. 1984. Toxicity of an overproduced foreign gene product in Escherichia coli and its use in plasmid vectors for the selection of transcription terminators. Gene 27: 161-172.
2. deBoer, H. A., Comstock, L. J., Vasser, M. 1983. The tac promoter: A functional hybrid derived from the trp and lac promoter. Proc. Natl. Acad. Sci. USA 80: 21-25.
3. Goeddel, D. V., Heyneker, H. L, Hozumi, T., Arentzen, R., Itakura, K., Yansura, D. G., Ross, M. J., Miozzari, G., Crea, R., Seeburg, P. H. 1979. Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone. Nature 281: 544-548.
4. Goeddel, D. V., Yelverton, E., Ulrich, A., Heyneker, H. L., Miozzari, G., Holmes, W., Seeburg, P. H., Dull, T., May, L., Stebing, N., Crea, R., Maeda, S., NcCandliss, R., Sloma, A., Tabor, J. M., Gross, M., Familetti, P. C., Pestka, S. 1980. Human leukocyte interferon produced by E. coli is biologically active. Nature 273: 411-416.
5. Gottschalk, G. 1986. Bacterial metabolism, 2nd edition. Springer-Verlag, New York.
6. Hortacsu, A., Ryu, D. D. D. 1990. Optimal temperature control policy for a two-stage recombinant fermentation process. Biotechnol. Prog. 6: 403-407.
7. Hughes, D. E., Curtis, J. E., Khosla, C., Bailey, J. E. 1989. A new oxygenregulated promoter for the expression of proteins in Escherichia coli. Biotechniques 7: 1026-1028.
8. Iuchi, S., Lin, E. C. C. 1987. The narL gene product activates the nitrate reductase operon and represses the fumarate reductase and trimethylamine N-oxide reductase operons in Escherichia coli. Proc. Natl. Acad. Sci. USA 84: 3091-3095.
9. Iuchi, S., Lin, E. C. C. 1991. Adaptation of Escherichia coli to regulatory conditions: Regulation of gene expression. Cell 66: 5-7.
10. Khosla, C., Bailey, J. E. 1989. Characterization of the oxygen-dependent promoter of the Vitreoscilla hemoglobin gene in Escherichia coli. J. Bacteriol. 171: 5995-6004.
11. Labes, M., Puhler, A., Simon, R. 1990. A new family of RSF1010-derived expression and lac-fusion broad-host-range vectors for gram-negative bacteria. Gene 89: 37-46.
12. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
13. Lee, S. B., Ryu, D. D. D., Seigel, R., Park, S. H. 1988. Performance of recombinant fermentation and evaluation of gene expression efficiency for gene product in two-stage continuous culture system. Biotechnol. Bioeng. 31: 805-820.
14. Lee, J. T., Cho, M. H., Hong, E. K., Kim, K. S., Lee, J. W. 1996. Characterization of the nar promoter to use as an inducible promoter. Biotechnol. Lett. 18: 129-134.
15. Li, S. F., DeMoss, J. A. 1987. Promoter region of the nar operon of Escherichia coli: Nucleotide sequence and transcription initiation signals. J. Bacteriol. 169: 4614-4620.
16. Li, S. F., DeMoss, J. A. 1988. Location of sequences in the nar promoter of Escherichia coli required for regulation by Fnr and NarL. J. Biol. Chem. 263: 13700-13705.
17. Li, S. F., Rabi, T., DeMoss, J. A. 1985. Delineation of two distinct domains in the 5′ region of the nar operon of Escherichia coli. J. Bacteriol. 164: 25-32.
18. Miller, J. H. 1972. Assay of β-galactosidase, pp. 352-355. In: Experiments in molecular genetics, 2nd edition. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
19. L promoter of coliphage λ. Gene 15: 81-93.
20. Seigel, R., Ryu, D. D. D. 1985. Kinetic study of instability of recombinant plasmid pPLc23trpA1 in E. coli using two-stage continuous culture system. Biotechnol. Bioeng. 27: 28-33.
21. Stewart, V., MacGregor, C. H. 1982. Nitrate reductase in Escherichia coli K-12: Involvement of chlC, chlE, and chlG loci, J. Bacteriol. 151: 788-799.
22. Walker, M. S., DeMoss, J.A. 1992. Role of alternative elements in transcription from the nar promoter of Escherichia coli. J. Bacteriol. 174: 1119-1123.