Characterization of promoter sequences for strong expression of groEx in Escherichia coli

Journal of Microbiology

Volumn 34, Issue 1, 1996, Pages 15-22

  Lee, Jung E ^a   Lim, Ssang T ^a   Ahn, Tae I ^a  

^a Seoul National University   (South Korea)

Author keywords

Gene expression, symbiosis; groE; Promoters

Indexed keywords

EID: 0007532404     PISSN: 12258873     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Abia, H., S. Adhya, and B. De Crombrugghe, 1981. Evidence for two functional gal promoters in intact Escherichia coli cells. J. Biol. Chem. 256: 11905-11910.
2. Ahn, T.I. and K.S. Ahn, 1987. Effect of the bacterial genetic components on the maintenance of the nucleus morphology of the symbiotic strain of Amoeba proteus. Kor. J. Genetics 9: 19-28.
3. Ahn, T.I., H.K. Leeu, I.H. Kwak, and K.W. Jeon, 1991. Nucleotide sequence and temperature-dependent expression of XgroEL gene isolated from symbiotic bacteria of Amoeba proteus. Endocyt. Cell Res. 8: 33-44.
4. Ahn, T.I., S.T. Lim, H.K. Leeu, J.E. Lee, and K.W. Jeon, 1994. A novel strong promoter of the groEx operon of symbiotic bacteria in Amoeba proteus. Gene 128: 43-49.
5. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl, 1990. Current protocols in molecular biology. Wiley, New York, NY.
6. Chirgwin, J.J., A.E. Przbyla, R.J. MacDonald, and W. J. Rutter, 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294.
7. Choi, E.Y., G.S. Ahn, and K.W. Jeon, 1991. Elevated levels of stress proteins associated with bacterial symbiosis in Amoeba proteus and soybean root nodule cells. BioSystems 25: 205-212.
8. Cowing, D.W., J.C.A. Bardwell, E.A. Craig, C. Woolford, R.W. Hendrix, and C.A. Gross, 1985. Consensus sequence for Escherichia coli heat shock gene promoters. Proc. Natl. Acad Sci. USA 82: 2679-2683.
9. E subunit of Escherichia coli RNA polymerase a second alternate factor involved in high-temperature expression. Genes Dev. 3: 1462-1471.
10. Gregory, J.P. and J.S. Tomas, 1990. Heat-shock proteins DnaK and GroEL facilitate export of LacZ hybrid proteins in E. coli. Nature 344: 882-884.
11. Grossman, A.D., J.W. Erickson, and C.A. Gross, 1984. The htpR gene product of E. coli is a sigma factor for heat shock promoters. Cell 38: 383-390.
12. Hara, E., T. Fukatsu, K. Takeda, M. Kengaku, C. Ohtaka, and H. Ishikawa, 1990. The predominant protein in an aphid endosymbiont is homologous to an E. coli heat shock protein. Symbiosis 8: 271-283.
13. Hawley, D.K. and W.R. McClure, 1983. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 11: 2237-2255.
14. Hemmingsen, S.M., C. Woolford, S.M. van der Vies, K. Tilly, D.T. Dennis, C.P. Georgepoulos, R.W. Hendrix, and R.J. Ellis, 1988. Homologous plant and bacterial proteins chaperone oligomeric protein assembly. Nature 333: 330-334.
15. Hoffman, P.S., L. Houston, and C.A. Butler, 1990. Legionella pneumophila htpAB heat shock operon: Nucleotide sequence and expression of the 60 kilodalton antigen in L pneumophila infected HeLa cells. Inf. Imm. 58: 3380-3387.
16. Jeon, K.W. and T.I. Ahn, 1978. Temperature sensitivity: A cell character determined by obligate endosymbionts in amoebas. Science 202: 635-637.
17. Jeon, K.W. and I.J. Lorch, 1967. Unusual intra-cellular bacterial infection in large, free-living amoebae. Exp. Cell Res. 48: 236-240.
18. Kunkel, T.A., 1985. Rapid and efficient site-specific mutagenesis without phenotype selection. Proc. Natl. Acad. Sci. USA 82: 488-492.
19. Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature 227: 680-685.
20. Lang, B.F. and G. Burger, 1990. A rapid high resolution DNA sequencing gel system. Anal. Biochem. 188: 176-180.
21. Lee, J.E., J.S. Hwang, and T.I. Ahn, 1994. Developing a temperature sensitive expression vector by application of groEx of symbiotic bacteria in Amoeba proteus. Kor. J. Microbiol. 32: 501-509.
22. Lee, S. and S. Racheed, 1990. A simple procedure for maximum yield of high quality plasmid DNA. Biotech 9: 676-679.
23. Li, M. and S.-L. Wong, 1992. Cloning and characterization of the groESL operon from Bacillus subtilis. J. Bacteriol. 174: 3981-3992.
24. Miller, J.H., 1972. Experiment in molecular genetics. Cold Spring Harbor Lab., Cold Spring Harbor, N. Y.
25. Narberhaus, F. and H. Ball, 1992. Cloning, sequencing, and molecular analysis of the groESL operon of Clostridium acetobutylicum. J. Bacteriol. 174: 3282-3289.
26. Neidhardt, F.C., R.A. VanBogelen, and E.T. Lau, 1983. Molecular cloning and expression of a gene that controls the high-temperature regulon of Escherichia coli. J. Bacteriol. 153: 597-603.
27. Sancar, G.B., A. Sancar, J.W. Little, and D. Rupp, 1982. The uvrB gene of Escherichia coli has both lex-A repressed and lex-A independent promoters. Cell 28: 523-530.
28. Webb, R., K.J. Reddy, and L.A. Sherman, 1990. Regulation and sequence of the Synechococcus sp. strain PCC7942 groESL operon, encoding a cyanobacterial chaperonin. J. Bacteriol. 172: 5079-5088.
29. 32. J. Bacteriol. 170: 3640-3649.