Structures of the Michaelis complex (1.2 Å) and the covalent acyl intermediate (2.0 Å) of cefamandole bound in the active sites of the mycobacterium tuberculosis β-lactamase K73A and E166A mutants

Biochemistry

Volumn 49, Issue 45, 2010, Pages 9685-9687

  Tremblay, Lee W ^a   Xu, Hua ^a   Blanchard, John S ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ACTIVE SITE RESIDUES; CATALYTIC MECHANISMS; COVALENTLY BOUND; DEACYLATION; LACTAMASES; MICHAELIS COMPLEX; MYCOBACTERIUM TUBERCULOSIS;

CHEMOTHERAPY;

BETA LACTAMASE; CEFAMANDOLE;

ACYLATION; ARTICLE; DEACYLATION; ENZYME ACTIVE SITE; HYDROGEN BOND; MUTATIONAL ANALYSIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE;

AMINO ACID SUBSTITUTION; BETA-LACTAMASES; CATALYTIC DOMAIN; CEFAMANDOLE; DNA, BACTERIAL; GENOME, BACTERIAL; KINETICS; MODELS, MOLECULAR; MYCOBACTERIUM TUBERCULOSIS; PROTEIN BINDING;

MYCOBACTERIUM TUBERCULOSIS;

EID: 78149457617     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi1015088     Document Type: Article

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