Bead-based protein-protein interaction assays for the analysis of Rho GTPase signaling

Journal of Molecular Recognition

Volumn 23, Issue 6, 2010, Pages 543-550

  Rimmele, Stefanie ^a   Gierschik, Peter ^b   Joos, Thomas O ^a,c   Schneiderhan Marra, Nicole ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b UNIVERSITY HOSPITAL ULM   (Germany)

^c EDI Experimental and Diagnostic Immunology GmbH   (Germany)

Author keywords

Bead based assays; P21 activated kinase; Post translational modification; Protein protein interaction; Rho GDI; Rho GTPases

Indexed keywords

BACTERIAL PROTEIN; CYSTEINE PROTEINASE; GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR; MICROSPHERE; P21 ACTIVATED KINASE; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITORS; YOPT PROTEIN, YERSINIA;

ARTICLE; BIOLOGICAL MODEL; CHEMISTRY; DRUG EFFECT; HUMAN; IMMUNOPRECIPITATION; METABOLISM; METHODOLOGY; PHYSIOLOGY; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PROCESSING; SIGNAL TRANSDUCTION;

BACTERIAL PROTEINS; CYSTEINE ENDOPEPTIDASES; GUANINE NUCLEOTIDE DISSOCIATION INHIBITORS; HUMANS; IMMUNOPRECIPITATION; MICROSPHERES; MODELS, BIOLOGICAL; P21-ACTIVATED KINASES; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RHO GTP-BINDING PROTEINS; SIGNAL TRANSDUCTION;

EID: 78049445650     PISSN: 09523499     EISSN: 10991352     Source Type: Journal    
DOI: 10.1002/jmr.1051     Document Type: Article

References (44)

1. Aepfelbacher M, Trasak C, Wilharm G, Wiedemann A, Trulzsch K, Krauss K, Gierschik P, Heesemann J. 2003. Characterization of YopT effects on Rho GTPases in Yersinia enterocolitica-infected cells. J. Biol. Chem. 278: 33217-33223.
2. Anderson JC, Wu N, Santoro SW, Lakshman V, King DS, Schultz PG. 2004. An expanded genetic code with a functional quadruplet codon. Proc. Natl. Acad. Sci. U. S. A. 101: 7566-7571.
3. Bagrodia S, Cerione RA. 1999. Pak to the future. Trends Cell Biol. 9: 350-355.
4. Bishop AL, Hall A. 2000. Rho GTPases and their effector proteins. Biochem. J. 348(2): 241-255.
5. Caruso ME, Jenna S, Beaulne S, Lee EH, Bergeron A, Chauve C, Roby P, Rual JF, Hill DE, Vidal M, Bosse R, Chevet E. 2005. Biochemical clustering of monomeric GTPases of the Ras superfamily. Mol. Cell Proteomics. 4: 936-944.
6. Cerione RA, Leonard D, Zheng Y. 1995. Purification of baculovirus-expressed Cdc42Hs. Methods Enzymol. 256: 11-15.
7. Cornelis GR. 2002a. Yersinia type III secretion: send in the effectors. J. Cell Biol. 158: 401-408.
8. Cornelis GR. 2002b. The Yersinia Ysc-Yop 'type III' weaponry. Nat. Rev. Mol. Cell Biol. 3: 742-752.
9. DeLean A, Munson PJ, Rodbard D. 1978. Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves. Am. J. Physiol. 235: E97-E102.
10. DerMardirossian C, Bokoch GM. 2005. GDIs: central regulatory molecules in Rho GTPase activation. Trends Cell Biol. 15: 356-363.
11. Dransart E, Olofsson B, Cherfils J. 2005. RhoGDIs revisited: novel roles in Rho regulation. Traffic. 6: 957-966.
12. Ellenbroek SI, Collard JG. 2007. Rho GTPases: functions and association with cancer. Clin. Exp. Metastasis. 24: 657-672.
13. Faure J, Dagher MC. 2001. Interactions between Rho GTPases and Rho GDP dissociation inhibitor (Rho-GDI). Biochimie 83: 409-414.
14. Forget MA, Desrosiers RR, Gingras D, Beliveau R. 2002. Phosphorylation states of Cdc42 and RhoA regulate their interactions with Rho GDP dissociation inhibitor and their extraction from biological membranes. Biochem. J. 361: 243-254.
15. Fueller F, Bergo MO, Young SG, Aktories K, Schmidt G. 2006. Endoproteolytic processing of RhoA by Rce1 is required for the cleavage of RhoA by Yersinia enterocolitica outer protein T. Infect. Immun. 74: 1712-1717.
16. Fueller F, Schmidt G. 2008. The polybasic region of Rho GTPases defines the cleavage by Yersinia enterocolitica outer protein T (YopT). Protein Sci. 17: 1456-1462.
17. Grizot S, Faure J, Fieschi F, Vignais PV, Dagher MC, Pebay-Peyroula E. 2001. Crystal structure of the Rac1-RhoGDI complex involved in NADPH oxidase activation. Biochemistry 40: 10007-10013.
18. Gururaj AE, Rayala SK, Kumar R. 2005. p21-Activated kinase signaling in breast cancer. Breast Cancer Res. 7: 5-12.
19. Hancock JF, Hall A. 1993. A novel role for RhoGDI as an inhibitor of GAP proteins. EMBO J. 12: 1915-1921.
20. Hoffman GR, Nassar N, Cerione RA. 2000. Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI. Cell 100: 345-356.
21. Hori Y, Kikuchi A, Isomura M, Katayama M, Miura Y, Fujioka H, Kaibuchi K, Takai Y. 1991. Post-translational modifications of the C-terminal region of the Rho protein are important for its interaction with membranes and the stimulatory and inhibitory GDP/GTP exchange proteins. Oncogene 6: 515-522.
22. Hwang SB, Lai MM. 1993. A unique conformation at the carboxyl terminus of the small hepatitis delta antigen revealed by a specific monoclonal antibody. Virology 193: 924-931.
23. Hwang SB, Lai MM. 1994. Isoprenylation masks a conformational epitope and enhances trans-dominant inhibitory function of the large hepatitis delta antigen. J. Virol. 68: 2958-2964.
24. Keep NH, Barnes M, Barsukov I, Badii R, Lian LY, Segal AW, Moody PC, Roberts GC. 1997. A modulator of rho family G proteins, rhoGDI, binds these G proteins via an immunoglobulin-like domain and a flexible N-terminal arm. Structure 5: 623-633.
25. Kikuchi A, Kuroda S, Sasaki T, Kotani K, Hirata K, Katayama M, Takai Y. 1992. Functional interactions of stimulatory and inhibitory GDP/GTP exchange proteins and their common substrate small GTP-binding protein. J. Biol. Chem. 267: 14611-14615.
26. Knaus UG, Bokoch GM. 1998. The p21Rac/Cdc42-activated kinases (PAKs). Int. J. Biochem. Cell Biol. 30: 857-862.
27. Knaus UG, Wang Y, Reilly AM, Warnock D, Jackson JH. 1998. Structural requirements for PAK activation by Rac GTPases. J. Biol. Chem. 273: 21512-21518.
28. Kreck ML, Freeman JL, Abo A, Lambeth JD. 1996. Membrane association of Rac is required for high activity of the respiratory burst oxidase. Biochemistry 35: 15683-15692.
29. Leonard D, Hart MJ, Platko JV, Eva A, Henzel W, Evans T, Cerione RA. 1992. The identification and characterization of a GDP-dissociation inhibitor (GDI) for the CDC42Hs protein. J. Biol. Chem. 267: 22860-22868.
30. Li R, Debreceni B, Jia B, Gao Y, Tigyi G, Zheng Y. 1999. Localization of the PAK1-, WASP-, and IQGAP1-specifying regions of Cdc42. J. Biol. Chem. 274: 29648-29654.
31. Morreale A, Venkatesan M, Mott HR, Owen D, Nietlispach D, Lowe PN, Laue. ED. 2000. Structure of Cdc42 bound to the GTPase binding domain of PAK. Nat. Struct. Biol. 7: 384-388.
32. Musha T, Kawata M, Takai Y. 1992. The geranylgeranyl moiety but not the methyl moiety of the smg-25A/rab3A protein is essential for the interactions with membrane and its inhibitory GDP/GTP exchange protein. J. Biol. Chem. 267: 9821-9825.
33. Robbe K, Otto-Bruc A, Chardin P, Antonny B. 2003. Dissociation of GDP dissociation inhibitor and membrane translocation are required for efficient activation of Rac by the Dbl homology-pleckstrin homology region of Tiam. J. Biol. Chem. 278: 4756-4762.
34. Sasaki T, Kato M, Takai Y. 1993. Consequences of weak interaction of rho GDI with the GTP-bound forms of rho p21 and rac p21. J. Biol. Chem. 268: 23959-23963.
35. Scheffzek K, Stephan I, Jensen ON, Illenberger D, Gierschik P. 2000. The Rac-RhoGDI complex and the structural basis for the regulation of Rho proteins by RhoGDI. Nat. Struct. Biol. 7: 122-126.
36. Shao F. 2008. Biochemical functions of Yersinia type III effectors. Curr. Opin. Microbiol. 11: 21-29.
37. Shao F, Merritt PM, Bao Z, Innes RW, Dixon JE. 2002. A Yersinia effector and a Pseudomonas avirulence protein define a family of cysteine proteases functioning in bacterial pathogenesis. Cell 109: 575-588.
38. Shao F, Vacratsis PO, Bao Z, Bowers KE, Fierke CA, Dixon JE. 2003. Biochemical characterization of the Yersinia YopT protease: cleavage site and recognition elements in Rho GTPases. Proc. Natl. Acad. Sci. U.S.A. 100: 904-909.
39. Shimizu T, Ihara K, Maesaki R, Kuroda S, Kaibuchi K, Hakoshima T. 2000. An open conformation of switch I revealed by the crystal structure of a Mg2+-free form of RHOA complexed with GDP. Implications for the GDP/GTP exchange mechanism. J. Biol. Chem. 275: 18311-18317.
40. Stofega MR, Sanders LC, Gardiner EM, Bokoch GM. 2004. Constitutive p21-activated kinase (PAK) activation in breast cancer cells as a result of mislocalization of PAK to focal adhesions. Mol. Biol. Cell. 15: 2965-2977.
41. Thapar R, Williams JG, Campbell SL. 2004. NMR characterization of full-length farnesylated and non-farnesylated H-Ras and its implications for Raf activation. J. Mol. Biol. 343: 1391-1408.
42. Van Aelst L, D'Souza-Schorey C. 1997. Rho GTPases and signaling networks. Genes Dev. 11: 2295-2322.
43. Wennerberg K, Der CJ. 2004. Rho-family GTPases: it's not only Rac and Rho (and I like it). J. Cell Sci. 117: 1301-1312.
44. Wennerberg K, Rossman KL, Der CJ. 2005. The Ras superfamily at a glance. J. Cell Sci. 118: 843-846.