Antimycin A-induced mitochondrial apoptotic cascade is mitigated by phenolic constituents of Phyllanthus amarus aqueous extract in Hep3B cells

Food and Chemical Toxicology

Volumn 48, Issue 12, 2010, Pages 3449-3457

  Guha, Gunjan ^a   Mandal, Tamoghna ^a   Rajkumar, V ^a   Ashok Kumar, R ^a  

^a VIT UNIVERSITY   (India)

Author keywords

AIF; Apoptosis; Cytochrome C; Mitochondrial membrane potential; MtDNA damage protection

Indexed keywords

8 HYDROXYDEOXYGUANOSINE; ANTIMYCIN A1; CYTOCHROME C; FLUORESCENT DYE; FREE RADICAL; HYDROGEN PEROXIDE; MITOCHONDRIAL DNA; PHENOL DERIVATIVE; PHYLLANTHUS AMARUS EXTRACT;

APOPTOSIS; AQUEOUS SOLUTION; ARTICLE; CELL AGING; CELL DEGENERATION; CELL MEMBRANE DEPOLARIZATION; CELL PROTECTION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA DAMAGE; DNA SEQUENCE; DOWN REGULATION; DRUG CYTOTOXICITY; DRUG MECHANISM; FLUORESCENCE ACTIVATED CELL SORTING; HEPATOMA CELL; HUMAN; HUMAN CELL; LIPID DEGRADATION; MEMBRANE DAMAGE; MEMBRANE STRUCTURE; MITOCHONDRIAL MEMBRANE POTENTIAL; OXIDATIVE STRESS; POLYMERASE CHAIN REACTION; PROTEIN DEGRADATION; PROTEIN SECRETION;

ANTI-BACTERIAL AGENTS; ANTIMYCIN A; APOPTOSIS; APOPTOSIS INDUCING FACTOR; BROMODEOXYURIDINE; CELL LINE; COLORING AGENTS; CYTOCHROMES C; DEOXYGUANOSINE; DNA, MITOCHONDRIAL; FLOW CYTOMETRY; HUMANS; INDICATORS AND REAGENTS; LIPID PEROXIDATION; MITOCHONDRIA; MITOCHONDRIAL MEMBRANES; MITOCHONDRIAL PROTEINS; PHENOLS; PHYLLANTHUS; PLANT EXTRACTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TETRAZOLIUM SALTS; THIAZOLES;

PHYLLANTHUS AMARUS;

EID: 78049445361     PISSN: 02786915     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fct.2010.09.020     Document Type: Article

References (48)

1. Alexandre A., Lehninger A.L. Bypasses of the antimycin a block of mitochondrial electron transport in relation to ubisemiquinone function. Biochim. Biophys. Acta 1984, 767(1):120-129.
2. Balaban R.S., Nemoto S., Finkel T. Mitochondria, oxidants, and aging. Cell 2005, 120(4):483-495.
3. Basoah A., Matthews P.M., Morten K.J. Rapid rates of newly synthesized mitochondrial protein degradation are significantly affected by the generation of mitochondrial free radicals. FEBS Lett. 2005, 579(28):6511-6517.
4. Beckman K.B., Ames B.N. Oxidative decay of DNA. J. Biol. Chem. 1997, 272(32):19633-19636.
5. Campo M.L., Kinnally K.W., Tedeschi H. The effect of antimycin A on mouse liver inner mitochondrial membrane channel activity. J. Biol. Chem. 1992, 267(12):8123-8127.
6. Chomyn A., Attardi G. MtDNA mutations in aging and apoptosis. Biochem. Biophys. Res. Commun. 2003, 304(3):519-529.
7. Cossarizza A., Baccarani Contri M., Kalashnikova G., Franceschi C. A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolcarbocyanine iodide (JC-1). Biochem. Biophys. Res. Commun. 1993, 197:40-45.
8. Costantini P., Chernyak B.V., Petronilli V., Bernardi P. Modulation of the mitochondrial permeability transition pore by pyridine nucleotides and dithiol oxidation at two separate sites. J. Biol. Chem. 1996, 271(12):6746-6751.
9. Daugas E., Nochy D., Ravagnan L., Loeffler M., Susin S.A., Zamzami N., Kroemer G. Apoptosis-inducing factor (AIF): a ubiquitous mitochondrial oxidoreductase involved in apoptosis. FEBS Lett. 2000, 476:118-123.
10. Faremi T.Y., Suru S.M., Fafunsoa M.A., Obioha U.E. Hepatoprotective potentials of Phyllanthus amarus against ethanol-induced oxidative stress in rats. Food Chem. Toxicol. 2008, 46(8):2658-2664.
11. Farris M.W., Chan C.B., Patel M., Van Houten B., Orrenius S. Role of mitochondria in toxic oxidative stress. Mol. Interventions 2005, 5:94-111.
12. Guha, G., Rajkumar, V., Ashok Kumar, R., Mathew, L., 2009. Therapeutic potential of polar and non-polar extracts of Cyanthillium cinereum in vitro. Evid. Based Complement. Alternat. Med. (Epub ahead of print; doi: 10.1093/ecam/nep155).
13. Guha G., Rajkumar V., Ashok Kumar R., Mathew L. Aqueous extract of Phyllanthus amarus inhibits chromium(VI)-induced toxicity in MDA-MB-435S cells. Food Chem. Toxicol. 2010, 48(1):396-401.
14. Halliwell B., Gutteridge J.M.C. Free Radicals in Biology and Medicine 1999, Oxford University Press, Oxford.
15. Han Y.H., Kim S.H., Kim S.Z., Park W.H. Intracellular GSH levels rather than ROS levels are tightly related to AMA-induced HeLa cell death. Chem. Biol. Interact. 2008, 171:67-78.
16. Harman D. Free radical theory of aging: consequences of mitochondrial aging. Age 1983, 6:86-94.
17. Hartree E.E. Determination of protein: A modification of the Lowry method that gives a linear photometric response. Anal. Biochem. 1972, 48:422-427.
18. Jiang X., Wang X. Cytochrome c-mediated apoptosis. Annu. Rev. Biochem. 2004, 73:87-106.
19. Kikkawa Y.S., Nakagawa T., Horie R.T., Ito J. Hydrogen protects auditory hair cells from free radicals. Neuroreport 2009, 20(7):689-694.
20. Krishnan K.J., Reeve A.K., Samuels D.C., Chinnery P.F., Blackwood J.K., Taylor R.W., Wanrooij S., Spelbrink J.N., Lightowlers R.N., Turnbull D.M. What causes mitochondrial DNA deletions in human cells?. Nat. Genet. 2008, 40(3):275-279.
21. Kuber V.V., Chawla J.L., Sane R.T. Thin layer chromatographic differentiation of Phyllanthus species and its implication in rational search for antihepatotoxic agents from them. Indian Drugs 1997, 34(1):36-42.
22. Lee H.C., Wei Y.H. Oxidative stress, mitochondrial DNA mutation, and apoptosis in aging. Exp. Biol. Med. 2007, 232:592-606.
23. Lister E., Wilson P. Measurement of total phenolics and ABTS assay for antioxidant activity 2001, Crop Research Institute, Lincoln, New Zealand, [personal communication].
24. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951, 193(1):265-275.
25. m) in apoptosis; an update. Apoptosis 2003, 8(2):115-128.
26. Martinez-Pastor F., Johannisson A., Gil J., Kaabi M., Anel L., Paz P., Rodriguez-Martinez H. Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen. Anim. Reprod. Sci. 2004, 84(1-2):121-133.
27. Moshi M.J., Uiso F.C., Mahunnah R.L.A., Malele S.R., Swai A.B.M. A Study of the effect of Phyllanthus amarus extracts on blood glucose in rabbits. Int. J. Pharmacol. 1997, 35(3):167-173.
28. Navarro A., Gómez C., Sánchez-Pino M.J., González H., Bández M.J., Boveris A.D., Boveris A. Vitamin E at high doses improves survival, neurological performance, and brain mitochondrial function in aging male mice. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2005, 289:R1392-R1399.
29. Nicolson G.L. Lipid replacement and antioxidant supplements to prevent membrane oxidation and restore mitochondrial function in metabolic syndrome and fatiguing illnesses. Townsend Lett. 2007, 286:112-120.
30. Othman A., Ismail A., Ghani N.A., Adenan I. Antioxidant capacity and phenolic content of cocoa beans. Food Chem. 2007, 100:1523-1530.
31. Ott M., Thyagarajan S.P., Gupta S. Phyllanthus amarus suppresses hepatitis B virus by interrupting interactions between HBV enhancer I and cellular transcription factors. Eur. J. Clin. Invest. 1997, 27(11):908-915.
32. Oxford, G., 2000. Mitochondrial DNA from Lumbriculus variegatus: Isolation and restriction digest analysis, in: Karcher, S.J. (Ed.), Tested studies for laboratory teaching, volume 22. Proceedings of the 22nd Workshop/Conference of the Association for Biology Laboratory Education (ABLE), pp. 64-80.
33. Panduri V., Weitzman S.A., Chandel N.S., Kamp D.W. Mitochondrial-derived free radicals mediate asbestos-induced alveolar epithelial cell apoptosis. Am. J. Physiol. 2004, 286(6):L1220-L1227.
34. Pastorino J.G., Tafani M., Rothman R.J., Marcinkeviciute A., Hoek J.B., Farber J.L. Functional consequences of the sustained or transient activation by Bax of the mitochondrial permeability transition pore. J. Biol. Chem. 1999, 274(44):31734-31739.
35. Petronilli V., Penzo D., Scorrano L., Bernardi P., Di Lisa F. The mitochondrial permeability transition, release of cytochrome c and cell death. Correlation with the duration of pore openings in situ. J. Biol. Chem. 2001, 276(15):12030-12034.
36. Pham N.A., Robinson B.H., Hedley D.W. Simultaneous detection of mitochondrial respiratory chain activity and reactive oxygen in digitonin-permeabilized cells using flow cytometry. Cytometry 2000, 41(4):245-251.
37. Raphael K.R., Sabu M.C., Kuttan R. Hypoglycemic effect of methanol extract of Phyllanthus amarus Schum and Thonn. on alloxan induced diabetes mellitus in rats and its relation with antioxidant potential. Indian J. Exp. Biol. 2002, 40:905-909.
38. Reers M., Smith T.W., Chen L.B. J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential. Biochem. 1991, 30:4480-4486.
39. Robak J., Marcinkiewicz E. Scavenging of reactive oxygen species as the mechanism of drug action. Pol. J. Pharmacol. 1995, 47:89-98.
40. Sadrazadeh S.M.H., Graf E., Panter S.S., Hallaway P.E., Eaten J.W. Hemoglobin, a biological Fenton reagent. J. Biol. Chem. 1984, 259:14354-14356.
41. Sane R.T., Kuber V.V., Chalissery M.S., Menon S. Hepatoprotection by Phyllanthus amarus and Phyllanthus debilis in CCl4-induced liver dysfunction. Curr. Sci. 1995, 68(12):1243-1246.
42. Smiley S.T., Reers M., Mottola-Hartshorn C., Lin M., Chen A., Smith T.W., Steele G.D., Chen L.B. Intracellular heterogeneity in mitochondrial membrane potential revealed by a J-aggregate-forming lipophilic cation JC-1. Proc. Natl. Acad. Sci. USA 1991, 88:3671-3675.
43. Strack D. Phenolic metabolism. Plant biochem. 1997, 387-416. Academic Press, San Diego. P.M. Dey, J.B. Harbourne (Eds.).
44. Tedesco I., Russo M., Russo P., Iacomino G., Russo G.L., Carraturo A., Faruolo C., Moio L., Palumbo R. Antioxidant effect of red wine polyphenols on red blood cells. J. Nutr. Biochem. 2000, 10:114-119.
45. Vladimir J., Vladimir B. In vitro assays for cell death determination. Arc. Oncol. 2008, 16(3-4):49-54.
46. Weislow O.S., Kiser R., Fine D.L., Bader J., Shoemaker R.H., Boyd M.R. New soluble-formazan assay for HIV-1 cytopathic effects: application to high-flux screening of synthetic and natural products for AIDS-antiviral activity. J. Natl. Cancer Inst. 1989, 81:577-586.
47. Yaar M. Lipid rafts participate in the generation of UVA-induced reactive oxygen species. J. Invest. Dermatol. 2002, 118:563-564.
48. Zhang H., Xu Y., Kamendulis L.M., Klaunig J.E. Morphological transformation by 8-hydroxy-2′-deoxyguanosine in Syrian hamster embryo (SHE) cells. Toxicol. Sci. 2000, 56:303-312.