Phyllanthus amarus suppresses hepatitis B virus by interrupting interactions between HBV enhancer I and cellular transcription factors

European Journal of Clinical Investigation

Volumn 27, Issue 11, 1997, Pages 908-915

  Ott, M ^a   Thyagarajan, S P ^b   Gupta, S ^a,c  

^a MARION BESSIN LIVER RESEARCH CENTER   (United States)

^b Dr A L M Postgrad Inst B   (India)

^c ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Hepatitis B virus; Liver; Medicinal plant; Treatment

Indexed keywords

PHYLLANTHUS AMARUS EXTRACT; TRANSCRIPTION FACTOR;

ANTIVIRAL ACTIVITY; ARTICLE; DRUG MECHANISM; ENHANCER REGION; GENETIC TRANSFECTION; HEPATITIS B VIRUS; HUMAN; HUMAN CELL; MEDICINAL PLANT; PLASMID; PRIORITY JOURNAL; VIRUS REPLICATION;

PHYLLANTHUS AMARUS;

EID: 0030780101     PISSN: 00142972     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2362.1997.2020749.x     Document Type: Article

References (37)

1. Perrillo RP, Mason AL. Therapy for hepatitis B virus infection. Gastroenterol Clin North Am 1994;23:581-601.
2. Venkateswaran PS, Millman I, Blumberg BS. Effects of an extract from Phyllanthus niruri on hepatitis B and woodchuck hepatitis B virus: in vitro and in vivo studies. Proc Natl Acad Sci USA 1987;84:274-8.
3. Thyagarajan SP, Subramanian S, Thirunalasundari T, Venkateswaran PS, Blumberg BS. Effect of Phyllanthus amarus in chronic carriers of hepatitis B virus. Lancet 1988;2:764-6.
4. Lee C-D, Ott M, Thyagarajan SP, Shafritz DA, Burk RD, Gupta S. Phyllanthus amarus downregulates hepatitis B virus mRNA transcription and replication. Eur J Clin Invest 1996;26:1069-1076.
5. Su H, Yee JK. Regulation of hepatitis B virus gene expression by its two enhancers. Proc Natl Acad Sci USA 1992;92:2708-712.
6. Antonucci TK, Rutter WJ. Hepatitis B virus (HBV) promoters are regulated by the HBV enhancer in a tissue-specific manner. J Virol 1989;63:579-83.
7. Ben-Levy RO, Faktor O, Berger I, Shaul Y. Cellular factors that interact with the hepatitis B virus enhancer. Mol Cell Biol 1989;9:1804-9.
8. Dikstein R, Faktor O, Shaul Y. Hierarchic and cooperative binding of the rat liver nuclear protein C/EBP at the hepatitis B virus enhancer. Mol Cell Biol 1990;10:4427-30.
9. Garcia AD, Ostapchuk P, Hearing P. Functional interaction of nuclear factors EF-C, HNF-4, and RXR alpha with hepatitis B virus enhancer I. J Virol 1993;67:3940-50.
10. Chen M, Hieng S, Qian X, Costa R, Ou JH. Regulation of hepatitis B virus ENI enhancer activity by hepatocyte-enriched transcription factor HNF3. Virology 1994;205:127-132.
11. Ishikawa T, Ganem D. The pre-S domain of the large viral envelope protein determines host range in avian hepatitis B viruses. Proc Natl Acad Sci USA 1995;92:6259-63.
12. Aragona E, Burk RD, Ott M, Shafritz DA, Gupta S. Cell-type specific mechanisms regulate hepatitis B virus transgene expression in liver and other organs. J Pathol 1996;180:441-9.
13. Kuo CJ, Conley PB, Chen L, Sladek FM, Darnell JE, Crabtree GR. A transcriptional hierarchy involved in mammalian cell-type specification. Nature 1992;355:457-61.
14. Vellanoweth RL, Suprakar PC, Roy AK. Transcription factors in development, growth, and aging. Lab Invest 1994;70:784-99.
15. Gupta S, Shafritz DA. Molecular biology of the hepatitis B virus. In: Walker WA, Durie PR, Hamilton JR, Walker-Smith JA, Watkins JB, eds. Pediatric gastrointestinal disease, pathophysiology, diagnosis, management. Philadelphia: BC Decker, 1991, 874-90.
16. Nakabayashi H, Taketa K, Miyano M, Yamane T, Sato J. Growth of human hepatoma cells lines with differentiated functions in chemically defined medium. Cancer Res 1982;42:3858-63.
17. Elfassi E. Broad specificity of the hepatitis B virus enhancer. Virology 1987;160:259-62.
18. Wu CH, Wilson JM, Wu GY. Targeting genes: delivery and persistent expression of a foreign gene driven by mammalian regulatory elements in vivo. J Biol Chem 1989;264:16985-7.
19. Mendel DB, Khavari PA, Conley PB et al. Characterization of a cofactor that regulates dimerization of a mammalian homeodomain protein. Science 1991;254:1762-7.
20. Samadani U, Porcella A, Pani L et al. Cytokine regulation of the liver transcription factor hepatocyte nuclear factor-3 beta is mediated by the C/EBP family and interferon regulatory factor 1. Cell Growth Diff 1995;6:879-90.
21. Gorman CM, Moffat LF, Howard BH. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol 1982;2:1044-51.
22. Gupta S, Vemuru RP, Yerneni PR, Jagtiani RK, Roy Chowdhury N, Shafritz DA, Burk RD, Roy Chowdhury J. Hepatocyte transplantation: Prolonged cell survival following ex-vivo manipulation and release from culture matrix components. Transplant Proc 1993;25:2370-2374.
23. Rosenthal N. Identification of regulatory elements of cloned genes with functional assays. Methods Enzymol 1987;152:704.
24. Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 1986;89:271-7.
25. Dignam JD, Lebovitz RM, Roeder RG. Eukaryotic gene transcription with purified components. Nucleic Acid Res 1983;11:1475-89.
26. Chang HK, Wang BY, Yuh CH, Wei CL, Ting LP. A liver-specific nuclear factor interacts with the promoter region of the large surface protein gene of human hepatitis B virus. Mol Cell Biol 1989;9:5189-97.
27. Wu KJ, Wilson DR, Shih C, Darlington GJ. The transcription factor HNF1 acts with C/EBP alpha to synergistically activate the human albumin promoter through a novel domain. J Biol Chem 1994;269:1177-82.
28. Sells MA, Chen ML, Acs G. Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA. Proc Natl Acad Sci USA 1987;84:274-8.
29. Tur-Kaspa R, Burk RD, Shaul Y, Shafritz DA. Hepatitis B virus DNA contains a glucocorticoid responsive element. Proc Natl Acad Sci USA 1986;83:1627-31.
30. Raney AK, Easton AJ, McLachlan A. Characterization of the minimal elements of the hepatitis B virus large surface antigen promoter. J Gen Virol 1994;75:2671-9.
31. De-Medina T, Faktor O, Shaul Y. The S promoter of hepatitis B virus is regulated by positive and negative elements. Mol Cell Biol 1988;8:2449-55.
32. Gustin K, Shapiro M, Lee W, Burk RD. Characterization of the role of individual protein binding motifs within the hepatitis B virus enhancer I on X promoter activity using linker scanning mutagenesis. Virology 1993;193:653-60.
33. Reith W, Ucla C, Barras E et al. RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel family of homodimeric and heterodimeric DNA-binding proteins. Mol Cell Biol 1994;14:1230-44.
34. Pei DQ, Shih CH. Transcriptional activation and repression by cellular DNA-binding protein C/EBP. J Virol 1990:64:1517-22.
35. Liaw YF, Tai D-I, Chu CM, Chen T-J. The development of cirrhosis in patients with chronic type B hepatitis: a prospective study. Hepatology 1988;8:493-6.
36. Cerny A, Fowler P, Brothers MA, Houghton M, Schlicht HJ, Chisari FV. Induction in vitro of a primary human antiviral cytotoxic T cell response. Eur J Immunol 1995;25:627-30.
37. Nayersina R, Fowler P, Guilhot S et al. HLA A2 restricted cytotoxic T lymphocyte responses to multiple hepatitis B surface antigen epitopes during hepatitis B virus infection. J Immunol 1993;150:4659-71.