Effects of X-ray irradiation on methylation levels of p16, MGMT and APC genes in sporadic colorectal carcinoma and corresponding normal colonic mucosa

Medical Science Monitor

Volumn 16, Issue 10, 2010, Pages

  Krakowczyk, Łukasz ^a,b   Blamek, Sławomir ^a   Strzelczyk, Joanna K ^b   Płachetka, Anna ^b   Maciejewski, Adam ^a   Półtorak, Stanisław ^a   Wiczkowski, Andrzej ^b  

^a INSTITUTE OF ONCOLOGY   (Poland)

^b MEDICAL UNIVERSITY OF SILESIA   (Poland)

Author keywords

APC genes; Colorectal cancer; DNA methylation; MGMT; P16

Indexed keywords

APC PROTEIN; GENOMIC DNA; METHYLATED DNA PROTEIN CYSTEINE METHYLTRANSFERASE; PROTEIN APC1A; PROTEIN APC1B; PROTEIN P16; TUMOR SUPPRESSOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CANCER RADIOTHERAPY; CLINICAL ARTICLE; COLON MUCOSA; COLORECTAL CARCINOMA; DNA METHYLATION; HUMAN; METHYLATION; RADIATION DOSE; X IRRADIATION; CONTROLLED STUDY; HUMAN TISSUE; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; RADIATION RESPONSE; TUMOR BIOPSY; ADULT; AGED; COLON; COLORECTAL TUMOR; COMPARATIVE STUDY; GENETICS; INTESTINE MUCOSA; METABOLISM; MIDDLE AGED; PATHOLOGY; RADIATION EXPOSURE; TUMOR SUPPRESSOR GENE; X RAY;

ADULT; AGED; AGED, 80 AND OVER; COLON; COLORECTAL NEOPLASMS; DNA METHYLATION; GENES, APC; GENES, P16; HUMANS; INTESTINAL MUCOSA; MIDDLE AGED; O(6)-METHYLGUANINE-DNA METHYLTRANSFERASE; X-RAYS;

EID: 78049289594     PISSN: 12341010     EISSN: 16433750     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Hardy RG, Meltzer SJ, Jankowski JA: ABC of colorectal cancer: Molecular basis for risk factors. BMJ, 2000; 321: 886-89
2. Lind G, Thorstensen L, Løvig T et al: A CpG island hypermethylation profile of primary colorectal carcinomas and colon cancer cell lines. Mol Cancer, 2004; 10: 1476-84
3. Mastrangelo D, De Francesco S, Di Leonardo A et al: The retinoblastoma paradigm revisited. Med Sci Monit, 2008; 14(12): RA231-40
4. Feinberg A, Vogelstein B: Hypomethylation distinguishes genes of some human cancers from their normal counterparts. Nature, 1983; 301: 89-92
5. Feinberg AP, Oshimura M, Barrett JC: Epigenetic Mechanisms in Human Disease. Cancer Res, 2002; 62: 6784-87
6. Baylin SB, Esteller M, Rountree MR et al: Aberrant patterns of DNA methylation, chromatin formation and gene expression in cancer. Hum Mol Genet, 2001; 10: 687-92
7. Costello J, Plass C: Methylation matters. J Med Genet, 2001; 38: 285-303
8. Xu X, Yu J, Zhang H et al: Methylation profile of the promoter CpG islands of 31 genes that may contribute to colorectal carcinogenesis. World J Gastroenterol, 2004; 10: 3441-54
9. Kalinich JF, Catravas GN, Snyder SL: The effect of gamma radiation on DNA methylation. Radiat Res, 1989; 117: 185-97
10. Kaplan MI, Limoli CL, Morgan WF: Perpetuating radiation-induced chromosomal instability. Radiat Oncol Investig, 1997; 5: 124-28
11. Smith EL, Nagar S, Kim GJ, Morgan W: Radiation - induced genomic instability: radiation quality and dose response. Health Phys, 2003; 85: 23-29
12. Kavalchuk O, Baulch JE: Epigenetic changes and nontargeted radiation effects-is there a link?. Environ Mol Mutag, 2008; 49: 16-25
13. Pogribny I, Raiche J, Slovack M, Kovalchuk O: Dose-dependence, sexand tissue-specifilicity, and persistence of radiation-induced genomic DNA methylation changes. Biochem Biophys Res Commun, 2004; 320: 1253-61
14. Barber RC, Hickenbotham P, Hatch T et al: Radiation-induced transgenerational alterations in genome stability and DANN damage. Oncogene, 2006; 25: 7336-42
15. Dubrova YE: Long-term genetic effects of radiation exposure. Mutat Rec, 2003; 544: 433-39
16. Krakowczyk Ł, Strzelczyk JK, Adamek B et al: Methylation of the MGMT and p16 genes in sporadic colorectal carcinoma and corresponding normal colonic mucosa. Med Sci Monit, 2008; 14(10): BR219-25
17. Herman JG, Graf JR, Myohanen S: Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA, 1996; 93: 9821-26
18. Qi J, Zhu YQ, Huang MF: Hypermethylation of CpG island in 06-methylguanine-DNA methylotransferase gene was associated with K-ras G to a mutation in colorectal tumor. World J Gastroenterol, 2005; 11: 2022-25
19. Nagar S, Smith LE, Morgan WF: Characterization of a novel epigenetic effect of ionizing radiation: The death-inducing effect. Cancer Res, 2003; 63: 324-28
20. BAGE hypomethylation, a new epigenetic biomarker for colon cancer detection. Cancer Epidemiol Biomarkers Prev, 2008; 17: 1374-79
21. Irizarry RA, Ladd-Acosta C, Wen B et al: The human colon cancer methylome shows similar hypo- and hypermethylation at conserved tissuespecific CpG island shores. Nat Genet, 2009; 41: 178-86
22. Pogribny I, Koturbash I, Tryndyak V et al: Fractionated Low-Dose Radiation exposure leads to accumulation of DNA damage and profound alterations in DNA and histone methylation in the murine thymus. Mol Cancer Res, 2005; 3: 553-61
23. Jeggo P, Lobrich M: Radiation-induced DNA damage responses. Radiat Prot Dosimetry, 2006; 122: 124-27